Mouse IL-13 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
在售SKU:70-EK213/2-48, 70-EK213/2-96, EK213
- 分子靶点:IL13
- 种属:小鼠
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本20μL
- 灵敏度:1.17pg/mL
- 检测范围:15.63-1000pg/mL
- 回收率:89%-112%
ELISA试剂盒详细信息
商品名 | 小鼠白介素13酶联免疫检测试剂盒 |
---|---|
种属 | 小鼠 |
靶点 | IL13 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本20μL |
灵敏度 | 1.17pg/mL |
线性范围 | 15.63-1000pg/mL |
精密度 | 板内变异系数:2.3%-3.0%;板间变异系数:2.1%-5.3% |
回收率 | 89%-112% |
平均回收率 | 102% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-13抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-13与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-13的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
IL13 分子靶点信息概述
- 分子名:IL13, interleukin 13
- 基因家族:Interleukins
- 别名:P600; IL-13; ALRH; BHR1; MGC116786; MGC116788; MGC116789
- 全称:allergic rhinitis; Bronchial hyperresponsiveness-1 (bronchial asthma)
IL13 分子靶点综述
白细胞介素13(IL-13)位于染色体5q31上,与IL-4具有30%的同源性和相似的结构。IL-13由许多细胞类型分泌产生,尤其是2型辅助性T细胞(Th2)。与密切相关的IL-4相似,IL-13对免疫细胞也具有多种功能。虽然IL-13主要与气道疾病的诱导相关,但是它还具有消炎特性。IL-13可诱导造血细胞的变化,但是这些效果与IL-4相比可能不那么重要。IL-13专门诱导寄生感染的器官的生理变化。它也可诱导过敏性肺部疾病的许多特征,包括气道高反应性、杯状细胞增殖和黏液高分泌,这些都会导致气道阻塞。此外,IL-13抑制原代培养衍生的巨噬细胞中HIV-1的复制,可作为体内单核细胞和巨噬细胞中抑制HIV感染的候选细胞因子。
小鼠 Mouse Il13 分子靶点信息
- 分子名:Il13, interleukin 13
- 别称:
- Il-13
- 基因序列:NCBI_Gene: 16163
- 蛋白序列:
小鼠 Mouse Il13 靶点分子功能(预测)
Predicted to enable interleukin-13 receptor binding activity. Involved in positive regulation of cold-induced thermogenesis and positive regulation of macrophage activation. Acts upstream of or within cellular response to cytokine stimulus; inflammatory response; and positive regulation of immune effector process. Located in cytoplasm; external side of plasma membrane; and extracellular space. Is expressed in gut; liver; male reproductive gland or organ; and thymus. Used to study atopic dermatitis. Human ortholog(s) of this gene implicated in several diseases, including allergic disease (multiple); autoimmune disease (multiple); diffuse scleroderma; lung disease (multiple); and nose disease (multiple). Orthologous to human IL13 (interleukin 13).
引用文献统计
该产品被引用的文献总数为:96
- Small Proline-Rich Protein 3 Regulates IL-33/ILC2 Axis to Promote Allergic Airway Inflammation
影响因子:8.786刊物:Frontiers in Immunology发表日期:2022/1/20 - ROS-responsive liposomes as an inhaled drug delivery nanoplatform for idiopathic pulmonary fibrosis treatment via Nrf2 signaling
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