Human Cystatin C ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
在售SKU:70-EK1114-48, 70-EK1114-96, EK1114
- 分子靶点:CST3
- 种属:人
- 样本类型:血清、血浆、细胞上清
- 检测样本体积:血清、血浆样本10μL
- 灵敏度:10.57pg/mL
- 检测范围:31.25-2000pg/mL
- 回收率:85%-115%
ELISA试剂盒详细信息
商品名 | 人半胱氨酸蛋白酶抑制剂C酶联免疫检测试剂盒 |
---|---|
种属 | 人 |
靶点 | CST3 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞上清 |
检测样本体积 | 血清、血浆样本10μL |
灵敏度 | 10.57pg/mL |
线性范围 | 31.25-2000pg/mL |
精密度 | 板内变异系数:3.3%-7.9%;板间变异系数:3.1%-6.2% |
回收率 | 85%-115% |
平均回收率 | 98% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人CystatinC抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的CystatinC与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中CystatinC的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
CST3 分子靶点信息概述
- 分子名:CST3, cystatin C
- 基因家族:Cystatins, type 2
- 全称:cystatin C (amyloid angiopathy and cerebral hemorrhage)
CST3 分子靶点综述
半胱氨酸蛋白酶抑制剂C属于半胱氨酸蛋白酶抑制剂超家族,是溶酶体蛋白酶的有效抑制剂,可能是半胱氨酸蛋白酶最重要的胞外抑制剂之一。它几乎存在于所有组织和体液中,包括血浆、尿液和脑脊液。Cystatin C通过调节半胱氨酸蛋白酶活性参与多种疾病过程。Cystatin C主要作为肾功能的生物标志物。最近,研究显示它在预测新发或恶化的心血管疾病中起作用。另外,它在淀粉样蛋白有关的脑疾病如阿尔茨海默氏病中发挥作用。
人 Human CST3 分子靶点信息
- 分子名:CST3, cystatin C
- 别称:
- ARMD11
- bA218C14.4 (cystatin C)
- cystatin 3
- cystatin C (amyloid angiopathy and cerebral hemorrhage)
- cystatin-3
- cystatin-C
- epididymis secretory protein Li 2
- gamma-trace
- HEL-S-2
- MGC117328
- neuroendocrine basic polypeptide
- post-gamma-globulin
- 基因序列:NCBI_Gene: 1471
- 蛋白序列:UniProtKB: P01034
人 Human CST3靶点分子功能(预测)
Enables several functions, including amyloid-beta binding activity; cysteine-type endopeptidase inhibitor activity; and identical protein binding activity. Involved in several processes, including negative regulation of blood vessel remodeling; negative regulation of catabolic process; and negative regulation of extracellular matrix disassembly. Located in Golgi apparatus; extracellular space; and plasma membrane. Implicated in CST3-related cerebral amyloid angiopathy and age related macular degeneration 11. Biomarker of several diseases, including artery disease (multiple); autoimmune disease of the nervous system (multiple); brain ischemia; obesity; and type 2 diabetes mellitus.
引用文献统计
该产品被引用的文献总数为:3
- Effects of continuous negative pressure suction combined with autologous platelet-rich gel on the levels of CRP, IL-6, wound healing and length of stay in clients with diabetic foot
影响因子:1.4刊物:TRANSFUSION AND APHERESIS SCIENCE发表日期:2024-08-10 - Clinical value of combined plasma brain natriuretic peptide and serum cystatin C measurement on the prediction of heart failure in patients after acute myocardial infarction
影响因子:2.3刊物:BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH发表日期:2023-09-22 - Microcystin-LR-Induced Interaction between M2 Tumor-Associated Macrophage and Colorectal Cancer Cell Promotes Colorectal Cancer Cell Migration through Regulating the Expression of TGF-β1 and CST3
影响因子:5.6刊物:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES发表日期:2023-06-23