Human CXCL5/ENA-78 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
在售SKU:70-EK1219-48, 70-EK1219-96, EK1219
- 分子靶点:CXCL5
- 种属:人
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本10μL
- 灵敏度:13.45pg/mL
- 检测范围:31.25-2000pg/mL
- 回收率:82%-125%
ELISA试剂盒详细信息
商品名 | 人趋化因子CXC配体5/上皮来源的中性粒细胞激活肽78酶联免疫检测试剂盒 |
---|---|
种属 | 人 |
靶点 | CXCL5 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本10μL |
灵敏度 | 13.45pg/mL |
线性范围 | 31.25-2000pg/mL |
精密度 | 板内变异系数:4.9%-6.4%;板间变异系数:2.8%-3.9% |
回收率 | 82%-125% |
平均回收率 | 101% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人CXCL5抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的CXCL5与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中CXCL5的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
CXCL5 分子靶点信息概述
- 分子名:CXCL5, C-X-C motif chemokine ligand 5
- 基因家族:Chemokine ligands
- 别名:ENA-78
- 曾用名:SCYB5
- 全称:small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78); chemokine (C-X-C motif) ligand 5
CXCL5 分子靶点综述
C-X-C基序趋化因子5(CXCL5),又名上皮来源的中性粒细胞激活肽78(ENA-78),是CXC趋化因子家族中的小分子量细胞因子。炎性细胞因子IL-1或TNF-α刺激细胞后可产生CXCL5,嗜酸性细胞也可表达CXCL5,而II型干扰素IFN-γ可抑制其表达。CXCL5通过与细胞表面趋化因子受体CXCR2相互作用来发挥其作用效果,可刺激具有血管生成特性的中性粒细胞的趋化性,并参与结缔组织重塑。CXCL5同样具有调节中性粒细胞稳态的作用。CXCL5在动脉粥样硬化(一种慢性炎症状态)中表达较高,但与中性粒细胞的浸润无关。相反,CXCL5可直接控制巨噬细胞的泡沫细胞形成,从而对动脉粥样硬化起保护作用。
人 Human CXCL5 分子靶点信息
- 分子名:CXCL5, C-X-C motif chemokine ligand 5
- 别称:
- C-X-C motif chemokine 5
- chemokine (C-X-C motif) ligand 5
- ENA-78
- ENA-78(1-78)
- epithelial-derived neutrophil activating protein 78
- epithelial-derived neutrophil-activating peptide 78
- epithelial-derived neutrophil-activating protein 78
- neutrophil-activating peptide ENA-78
- neutrophil-activating protein 78
- SCYB5
- small inducible cytokine B5
- small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78)
- small-inducible cytokine B5
- 基因序列:NCBI_Gene: 6374
- 蛋白序列:UniProtKB: P42830
人 Human CXCL5靶点分子功能(预测)
Enables identical protein binding activity. Predicted to be involved in several processes, including cellular response to lipopolysaccharide; chemokine-mediated signaling pathway; and neutrophil chemotaxis. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Implicated in idiopathic pulmonary fibrosis. Biomarker of several diseases, including common cold; diffuse scleroderma; hyperhomocysteinemia; lung disease (multiple); and sinusitis.
引用文献统计
该产品被引用的文献总数为:6
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