Rat Adiponectin/Acrp30 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥2,000.00 – ¥3,400.00
在售SKU:70-EK395-48, 70-EK395-96, EK395
- 分子靶点:ADIPOQ
- 种属:大鼠
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本5μL
- 灵敏度:0.46pg/mL
- 检测范围:78.13-5,000pg/mL
- 回收率:80%-110%
ELISA试剂盒详细信息
商品名 | Rat Adiponectin/Acrp30 酶联免疫检测试剂盒 |
---|---|
种属 | 大鼠 |
靶点 | ADIPOQ |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本5μL |
灵敏度 | 0.46pg/mL |
线性范围 | 78.13-5,000pg/mL |
精密度 | 板内变异系数:1.6%-4.6%;板间变异系数:1.6%-3.5% |
回收率 | 80%-110% |
平均回收率 | 105% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的待测物质与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物 TMB,避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在 450nm 波长(参考波长 570 - 630nm)测定吸光度值。 |
分子信息
ADIPOQ 分子靶点信息概述
- 分子名:ADIPOQ, adiponectin, C1Q and collagen domain containing
- 基因家族:C1q and TNF related
- 别名:ACRP30; apM1; GBP28; adiponectin
- 曾用名:ACDC
- 全称:adipose most abundant gene transcript 1; adiponectin precursor; adipocyte, C1Q and collagen domain containing
ADIPOQ 分子靶点综述
脂联素,又称脂肪细胞补体相关蛋白30(Acrp30),参与调节葡萄糖水平和脂肪酸分解。它组装成不同的复合物包括三聚体、六聚体和高阶寡聚体结构,进而可能影响生物活性。脂联素是一种蛋白质激素,调节许多代谢过程,包括葡萄糖调节和脂肪酸氧化。脂肪组织(以及妊娠期的胎盘)专门分泌脂联素到血液中,因此相对于其它许多激素,血浆中的脂联素含量很丰富。脂联素可能具有抗动脉粥样硬化和消炎的作用,可负向调节粒单祖细胞的生长和TNF-α在巨噬细胞中的产生。监测一些病理状态下的血清脂联素水平,如2型糖尿病、肥胖、动脉粥样硬化和非酒精性脂肪肝疾病,可为我们了解脂联素提供更详细的见解。
大鼠 Rat Adipoq 分子靶点信息
- 分子名:Adipoq, adiponectin, C1Q and collagen domain containing
- 别称:
- Acdc
- Acrp30
- Adid
- adipocyte complement related protein of 30 kDa
- adiponectin
- adiponectin d
- 基因序列:NCBI_Gene: 246253
- 蛋白序列:
大鼠 Rat Adipoq 靶点分子功能(预测)
Enables identical protein binding activity. Involved in several processes, including cellular response to organonitrogen compound; positive regulation of glycogen (starch) synthase activity; and positive regulation of protein phosphorylation. Located in extracellular space. Part of protein-containing complex. Used to study brain infarction. Biomarker of several diseases, including end stage renal disease; fatty liver disease (multiple); glucose metabolism disease (multiple); hyperthyroidism; and obesity. Human ortholog(s) of this gene implicated in several diseases, including carcinoma (multiple); cardiovascular system disease (multiple); non-alcoholic fatty liver disease; primary open angle glaucoma; and type 2 diabetes mellitus. Orthologous to human ADIPOQ (adiponectin, C1Q and collagen domain containing).
引用文献统计
该产品被引用的文献总数为:7
- Reconfiguration of Gut Microbiota and Reprogramming of Liver Metabolism with Phycobiliproteins Bioactive Peptides to Rehabilitate Obese Rats
影响因子:6.706刊物:Nutrients发表日期:2022/9/2 - α-Mangostin Alleviated Inflammation in Rats With Adjuvant-Induced Arthritis by Disrupting Adipocytes-Mediated Metabolism-Immune Feedback
影响因子:5.811刊物:Frontiers in Pharmacology发表日期:2021/7/7 - Consumption of Saturated Fatty Acids-Rich Lard Benefits Recovery of Experimental Arthritis by Activating PPAR-γ
影响因子:6.575刊物:MOLECULAR NUTRITION & FOOD RESEARCH发表日期:2022/11/25 - Qing-Luo-Yin Alleviated Monocytes/Macrophages-Mediated Inflammation in Rats with Adjuvant-Induced Arthritis by Disrupting Their Interaction with (Pre)-Adipocytes Through PPAR-γ Signaling
影响因子:4.162刊物:Drug Design Development and Therapy发表日期:2021/7/16 - Heat-killed Bifidobacterium longum BBMN68 and inulin protect against high-fat diet-induced obesity by modulating gut microbiota
影响因子:4刊物:Frontiers in Nutrition发表日期:2024-08-14 - White adipose tissue, a novel antirheumatic target: Clues from its secretory capability and adipectomy-based therapy
影响因子:6.8刊物:BRITISH JOURNAL OF PHARMACOLOGY发表日期:2024-04-21 - Α-Mangostin Alleviated Inflammation in Rats with Adjuvant-Induced Arthritis by Disrupting Adipocytes-Mediated Metabolism-Immune Feedback
刊物:Frontiers in pharmacology 12, 692806 (2021)