EK101B

Human IL-1β ELISA Kit检测试剂盒(酶联免疫吸附法)

¥1,600.00¥2,650.00

  • 分子靶点:IL1B, IL1-β, IL1F2
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:20 μL
  • 灵敏度:0.15 pg/mL
  • 检测范围:3.91 pg/mL - 250 pg/mL
  • 回收率:94% - 113%

在售SKU:70-EK101B-48, 70-EK101B-96, 人白介素1β, 白介素-1β, 白介素-1B, 人, 白介素, 1β, 1B


描述

商品名

Human IL-1β ELISA Kit (人白介素1β ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:4.7% - 6.1%;板间变异系数:4.1% - 6.6%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

20 μL

灵敏度

0.15 pg/mL

检测范围

3.91 pg/mL - 250 pg/mL

回收率

94% - 113%

平均回收率

1.05

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗IL-1β单克隆抗体的96孔聚苯乙烯酶标板
  • 人IL-1β冻干标准品
  • IL-1β检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-1β抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-1β与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-1β的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-1β ELISA Kit (人白介素1β ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 IL1B, IL1-β, IL1F2 靶点信息

IL1B 分子靶点信息概述

  • 分子名:IL1B, interleukin 1 beta
  • 基因家族:Interleukins
  • 别名:IL1F2; IL-1B; IL1-BETA

IL1B 分子靶点综述

白细胞介素1(IL-1)是17kDa的胞外多肽,分为IL-1α和IL-1β两种蛋白。IL-1β是活化的巨噬细胞分泌的蛋白前体,通过caspase1(CASP1/ICE)水解产生的活化形式。该细胞因子是炎症反应的重要介导者,参与许多细胞活性,包括细胞增殖、分化和凋亡。IL-1是一类重要的蛋白家族,它们由生物活化的单核细胞所衍生,参与炎症反应和免疫应答。IL-1β表达升高会产生各种不同的自身炎症综合征,最显著的是单基因条件称为CAPS,是由于触发IL-1β加工的炎性体受体NLRP3发生突变而引起的。本试剂盒用来检测人IL-1β的成熟形式和未裂解的前体形式。

人 Human IL1B 分子靶点信息

  • 分子名:IL1B, interleukin 1 beta
  • 别称:
    • catabolin
    • IL-1
    • IL-1 beta
    • IL1-BETA
    • IL1beta
    • IL1F2
    • interleukin 1, beta
    • interleukin 1beta
    • interleukin-1 beta
    • preinterleukin 1 beta
    • pro-interleukin-1-beta
  • 基因序列:NCBI_Gene: 3553
  • 蛋白序列:UniProtKB: P01584

人 Human IL1B靶点分子功能(预测)

Enables cytokine activity; integrin binding activity; and protein domain specific binding activity. Involved in several processes, including positive regulation of cytokine production; positive regulation of nitrogen compound metabolic process; and regulation of signal transduction. Acts upstream of or within cellular response to organic substance and cellular response to xenobiotic stimulus. Located in extracellular space. Implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); bronchial disease (multiple); lung disease (multiple); and nose disease (multiple).

操作步骤


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引用文献


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    Melatonin attenuates LPS-induced pyroptosis in acute lung injury by inhibiting NLRP3-GSDMD pathway via activating Nrf2/HO-1 signaling axis 

  2. 细胞培养上清

    Transient Receptor Potential Ankyrin 1 Contributes to the Atp-Elicited Oxidative Stress and Inflammation in Thp-1-Derived Macrophage 

  3. 细胞培养上清 脐带静脉内皮细胞 血清

    Cd137 Signaling Promotes Endothelial Apoptosis by Inhibiting Nrf2 Pathway, and Upregulating Nf-Κb Pathway 

  4. Transient Receptor Potential Ankyrin 1 Contributes to the Lysophosphatidylcholine-Induced Oxidative Stress and Cytotoxicity in Oln-93 Oligodendrocyte 

  5. 细胞培养上清

    Withaphysalins from Medicinal and Edible Physalis minima and Their Anti-inflammatory Activities 

  6. Dfmg Attenuates the Activation of Macrophages Induced by Co?Culture with Lpc?Injured Huve?12 Cells Via the Tlr4/Myd88/Nf?Κb Signaling Pathway 

  7. Expression of Rankl by Peripheral Neutrophils and Its Association with Bone Mineral Density in Copd 

  8. Effect of Dexmedetomidine on Early Postoperative Cognitive Dysfunction and Peri-Operative Inflammation in Elderly Patients Undergoing Laparoscopic Cholecystectomy 

  9. Levels of Interleukin-1β, Interleukin-18, and Tumor Necrosis Factor-Α in Cerebrospinal Fluid of Aneurysmal Subarachnoid Hemorrhage Patients May Be Predictors of Early Brain Injury and Clinical Prognosis 

  10. Fluorescence Quenching Nanoprobes Dedicated to in Vivo Photoacoustic Imaging and High-Efficient Tumor Therapy in Deep-Seated Tissue 

  11. Ginsenoside Rg3 Suppresses the Nlrp3 Inflammasome Activation through Inhibition of Its Assembly 

  12. Helicobacter Pylori Tumor Necrosis Factor-Α Inducing Protein Promotes Cytokine Expression Via Nuclear Factor-Κb 

  13. Cardioprotective Effect of Anisodamine against Myocardial Ischemia Injury and Its Influence on Cardiomyocytes Apoptosis 

  14. Effects of Intranasal Administration with Bacillus Subtilis on Immune Cells in the Nasal Mucosa and Tonsils of Piglets 

  15. Paeoniflorin Down-Regulates Atp-Induced Inflammatory Cytokine Production and P2x7r Expression on Peripheral Blood Mononuclear Cells from Patients with Primary Sj?gren's Syndrome 

  16. Diagnostic and Predictive Levels of Calcium-Binding Protein A8 and Tumor Necrosis Factor Receptor-Associated Factor 6 in Sepsis-Associated Encephalopathy: A Prospective Observational Study 

  17. Nlrp3 Inflammasome Is Required for Apoptosis of Aggregatibacter Actinomycetemcomitans-Infected Human Osteoblastic Mg63 Cells 

  18. Anti-Inflammatory Activities of Phospholipase C Inhibitor U73122: Inhibition of Monocyte-to-Macrophage Transformation and Lps-Induced Pro-Inflammatory Cytokine Expression 

  19. Toll-Like Receptor 4 Signaling Pathway in the Protective Effect of Pioglitazone on Experimental Immunoglobulin a Nephropathy 

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