Mouse Leptin ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
在售SKU:70-EK297-48, 70-EK297-96, EK297, 小鼠瘦素 小鼠 瘦素
- 分子靶点:LEP
- 种属:小鼠
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本10μL
- 灵敏度:2.48pg/mL
- 检测范围:62.5-4000pg/mL
- 回收率:73%-120%
ELISA试剂盒详细信息
商品名 | 小鼠瘦素酶联免疫检测试剂盒 |
---|---|
种属 | 小鼠 |
靶点 | LEP |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本10μL |
灵敏度 | 2.48pg/mL |
线性范围 | 62.5-4000pg/mL |
精密度 | 板内变异系数:3.9%-5.9%;板间变异系数:2.0%-2.1% |
回收率 | 73%-120% |
平均回收率 | 102% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠Leptin抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的Leptin与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中Leptin的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
LEP 分子靶点信息概述
- 分子名:LEP, leptin
- 基因家族:Neuropeptides
- 曾用名:OBS; OB
- 全称:leptin (murine obesity homolog); leptin (obesity homolog, mouse)
LEP 分子靶点综述
瘦素是由脂肪细胞分泌的蛋白质类激素,可通过抑制饥饿来调控能量平衡。除了白色脂肪组织可以分泌瘦素外,褐色脂肪、胎盘、卵巢、骨骼肌、胃、乳腺上皮细胞、骨髓和P/D1细胞也能产生瘦素。瘦素和饥饿激素都作用于下丘脑弓状核的受体,以此来调节食欲,获得能量平衡。瘦素的基本功能是调节脂肪储存,但它在其它生理代谢过程中同样发挥作用,然而许多其它功能还未明确。研究表明瘦素与肥胖和减肥有关联。
小鼠 Mouse Lep 分子靶点信息
- 分子名:Lep, leptin
- 别称:
- ob
- obese
- 基因序列:NCBI_Gene: 16846
- 蛋白序列:
小鼠 Mouse Lep 靶点分子功能(预测)
Enables DNA binding activity and growth factor activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of cytokine production; and positive regulation of signal transduction. Acts upstream of or within several processes, including insulin secretion; monocarboxylic acid metabolic process; and regulation of insulin secretion. Located in cytoplasm and extracellular space. Is expressed in several structures, including adipose tissue; alimentary system; central nervous system; early conceptus; and genitourinary system. Used to study abdominal obesity-metabolic syndrome (multiple); fatty liver disease (multiple); obesity; and type 2 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including alcohol dependence; congenital leptin deficiency; liver disease (multiple); lung disease (multiple); and type 2 diabetes mellitus. Orthologous to human LEP (leptin).
引用文献统计
该产品被引用的文献总数为:19
- Limits to sustained energy intake. XXX. Constraint or restraint? Manipulations of food supply show peak food intake in lactation is constrained
影响因子:3.014刊物:JOURNAL OF EXPERIMENTAL BIOLOGY发表日期:2020/4/15 - In vivo self-assembled small RNAs as a new generation of RNAi therapeutics
影响因子:25.617刊物:CELL RESEARCH发表日期:2021/3/29 - Actin related protein 2/3 complex subunit 1 up-regulation in the hypothalamus prevents high-fat diet induced obesity
影响因子:3.698刊物:EUROPEAN JOURNAL OF NEUROSCIENCE发表日期:2022/11/16 - Rutin and Its Combination With Inulin Attenuate Gut Dysbiosis, the Inflammatory Status and Endoplasmic Reticulum Stress in Paneth Cells of Obese Mice Induced by High-Fat Diet
影响因子:4.019刊物:Frontiers in Microbiology发表日期:2018/11/5 - Gut microbiota mediates the anti-obesity effect of calorie restriction in mice
影响因子:4.122刊物:Scientific Reports发表日期:2018/8/29 - GPAT3 deficiency alleviates insulin resistance and hepatic steatosis in a mouse model of severe congenital generalized lipodystrophy
影响因子:4.544刊物:HUMAN MOLECULAR GENETICS发表日期:2019/12/24 - Glucocorticoid exposure affects female fertility by exerting its effect on the uterus but not on the oocyte: lessons from a hypercortisolism mouse model
影响因子:4.99刊物:HUMAN REPRODUCTION发表日期:2018/11/2 - Effects of ambient temperatures between 5 and 35 °C on energy balance, body mass and body composition in mice
影响因子:8.568刊物:Molecular Metabolism发表日期:2022/7/20 - Weight-Reducing Effect of Lactobacillus Plantarum ZJUFT17 Isolated from Sourdough Ecosystem
影响因子:4.546刊物:Nutrients发表日期:2020/4/1 - Fibroblast transdifferentiation promotes conversion of M1 macrophages and replenishment of cardiac resident macrophages following cardiac injury in mice
影响因子:4.404刊物:EUROPEAN JOURNAL OF IMMUNOLOGY发表日期:2020/2/18 - Remodeling on adipocytic physiology of organophosphorus esters in mature adipocytes
影响因子:9.988刊物:ENVIRONMENTAL POLLUTION发表日期:2022/4/11 - Legumain-deficient macrophages regulate inflammation and lipid metabolism in adipose tissues to protect against diet-induced obesity
影响因子:3.8刊物:MOLECULAR AND CELLULAR ENDOCRINOLOGY发表日期:2024-05-28 - Epigallocatechin gallate suppresses mitotic clonal expansion and adipogenic differentiation of preadipocytes through impeding JAK2/STAT3-mediated transcriptional cascades
影响因子:6.7刊物:PHYTOMEDICINE发表日期:2024-03-22 - SIRT1 inhibitors within Qing-Luo-Yin alleviated white adipose tissues-mediated inflammation in antigen-induced arthritis mice
影响因子:7.9刊物:PHYTOMEDICINE发表日期:2023-10-04 - Chinese chestnut shell polyphenol extract regulates the JAK2/STAT3 pathway to alleviate high-fat diet-induced, leptin-resistant obesity in mice
影响因子:6.1刊物:Food & Function发表日期:2023-04-26 - CircHOMER1 inhibits porcine adipogenesis via the miR-23b/SIRT1 axis
影响因子:4.8刊物:FASEB JOURNAL发表日期:2023-02-21 - Rutin and Its Combination with Inulin Attenuate Gut Dysbiosis, the Inflammatory Status and Endoplasmic Reticulum Stress in Paneth Cells of Obese Mice Induced by High-Fat Diet
刊物:Frontiers in microbiology 9, 2651 (2018) - Glucocorticoid Exposure Affects Female Fertility by Exerting Its Effect on the Uterus but Not on the Oocyte: Lessons from a Hypercortisolism Mouse Model
刊物:Human reproduction (Oxford, England) 33, 2285-2294 (2018) - Human Epidermal Growth Factor Receptor-2 Antibodies Enhance the Specificity and Anticancer Activity of Light-Sensitive Doxorubicin-Labeled Liposomes
刊物:Biomaterials 57, 1-11 (2015)