Human IL-5 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
在售SKU:70-EK105-48, 70-EK105-96, EK105, 人白细胞介素5, 白细胞介素5, 白细胞介素, 人, 白介素, 5
- 分子靶点:IL5
- 种属:人
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:50μL
- 灵敏度:0.76pg/mL
- 检测范围:7.81-500pg/mL
- 回收率:91%-107%
ELISA试剂盒详细信息
商品名 | 人白介素5酶联免疫检测试剂盒 |
---|---|
种属 | 人 |
靶点 | IL5 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 50μL |
灵敏度 | 0.76pg/mL |
线性范围 | 7.81-500pg/mL |
精密度 | 板内变异系数:2.5%-4.0%;板间变异系数:3.2%-6.1% |
回收率 | 91%-107% |
平均回收率 | 99% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-5抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-5与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-5的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
IL5 分子靶点信息概述
- 分子名:IL5, interleukin 5
- 基因家族:Interleukins
- 别名:IL-5; EDF; TRF
- 全称:interleukin-5; T-cell replacing factor; B cell differentiation factor I; eosinophil differentiation factor; colony-stimulating factor, eosinophil; interleukin 5 (colony-stimulating factor, eosinophil)
IL5 分子靶点综述
白细胞介素5(IL-5)是造血家族Th2细胞因子成员,由115个氨基酸组成。与该细胞因子家族其它成员(IL-3和GM-CSF)不同,该糖蛋白的活化形式是同源二聚体。IL-5由Th2细胞和肥大细胞分泌。它对B细胞以及其它类型的细胞产生的生物学活性表明,IL-5对Th2细胞的活性具有重要的功能性作用。IL-5通过与其受体结合,刺激B细胞生长,提高免疫球蛋白分泌。它也是活化嗜酸性粒细胞的关键因子。IL-5一直以来与多种变态疾病相关,包括变应性鼻炎和哮喘,在循环组织、气管组织中观察到大幅增加的IL-5,而痰嗜酸性粒细胞则减少。
人 Human IL5 分子靶点信息
- 分子名:IL5, interleukin 5
- 别称:
- B cell differentiation factor I
- B-cell differentiation factor I
- colony-stimulating factor, eosinophil
- EDF
- eosinophil differentiation factor
- IL-5
- interleukin-5
- T-cell replacing factor
- TRF
- 基因序列:NCBI_Gene: 3567
- 蛋白序列:UniProtKB: P05113
人 Human IL5靶点分子功能(预测)
Predicted to enable cytokine activity; growth factor activity; and interleukin-5 receptor binding activity. Involved in positive regulation of podosome assembly. Located in extracellular region. Implicated in asthma. Biomarker of several diseases, including Churg-Strauss syndrome; Henoch-Schoenlein purpura; arthritis (multiple); asthma (multiple); and lymphoid leukemia (multiple).
引用文献统计
该产品被引用的文献总数为:13
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