Mouse IL-5 High Sensitivity ELISA Kit检测试剂盒(酶联免疫吸附法)
¥2,000.00 – ¥3,400.00
在售SKU:70-EK205HS-48, 70-EK205HS-96
- 分子靶点:IL5
- 种属:小鼠
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本20μL
- 灵敏度:0.14pg/mL
- 检测范围:1.56-100pg/mL
- 回收率:104%-107%
ELISA试剂盒详细信息
商品名 | 小鼠白介素5高敏酶联免疫检测试剂盒 |
---|---|
种属 | 小鼠 |
靶点 | IL5 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本20μL |
灵敏度 | 0.14pg/mL |
线性范围 | 1.56-100pg/mL |
精密度 | 板内变异系数:4.8%-5.0%;板间变异系数:3.0%-5.3% |
回收率 | 104%-107% |
平均回收率 | 105% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-5抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的IL-5与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入信号增强剂孵育,洗涤去除未结合的物质后,再次加入Streptavidin-HRP。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-5的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
IL5 分子靶点信息概述
- 分子名:IL5, interleukin 5
- 基因家族:Interleukins
- 别名:IL-5; EDF; TRF
- 全称:interleukin-5; T-cell replacing factor; B cell differentiation factor I; eosinophil differentiation factor; colony-stimulating factor, eosinophil; interleukin 5 (colony-stimulating factor, eosinophil)
IL5 分子靶点综述
白细胞介素5(IL-5)是造血家族Th2细胞因子成员,由115个氨基酸组成。与该细胞因子家族其它成员(IL-3和GM-CSF)不同,该糖蛋白的活化形式是同源二聚体。IL-5由Th2细胞和肥大细胞分泌。它对B细胞以及其它类型的细胞产生的生物学活性表明,IL-5对Th2细胞的活性具有重要的功能性作用。IL-5通过与其受体结合,刺激B细胞生长,提高免疫球蛋白分泌。它也是活化嗜酸性粒细胞的关键因子。IL-5一直以来与多种变态疾病相关,包括变应性鼻炎和哮喘,在循环组织、气管组织中观察到大幅增加的IL-5,而痰嗜酸性粒细胞则减少。
小鼠 Mouse Il5 分子靶点信息
- 分子名:Il5, interleukin 5
- 别称:
- Il-5
- 基因序列:NCBI_Gene: 16191
- 蛋白序列:
小鼠 Mouse Il5 靶点分子功能(预测)
Enables cytokine activity. Involved in positive regulation of DNA-binding transcription factor activity; positive regulation of immunoglobulin production; and positive regulation of transcription, DNA-templated. Acts upstream of or within positive regulation of peptidyl-tyrosine phosphorylation. Located in extracellular space. Is expressed in colon; thymus; and thymus primordium. Human ortholog(s) of this gene implicated in asthma. Orthologous to human IL5 (interleukin 5).
引用文献统计
该产品被引用的文献总数为:57
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