Human GM-CSF High Sensitivity ELISA Kit检测试剂盒(酶联免疫吸附法)
¥2,000.00 – ¥3,400.00
在售SKU:70-EK163HS-48, 70-EK163HS-96
- 分子靶点:CSF2
- 种属:人
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本20μL
- 灵敏度:0.07pg/mL
- 检测范围:0.16-10pg/mL
- 回收率:80%-101%
ELISA试剂盒详细信息
商品名 | 人 GM-CSF高敏酶联免疫检测试剂盒 |
---|---|
种属 | 人 |
靶点 | CSF2 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本20μL |
灵敏度 | 0.07pg/mL |
线性范围 | 0.16-10pg/mL |
精密度 | 板内变异系数:4.6%-5.5%;板间变异系数:4.8%-5.5% |
回收率 | 80%-101% |
平均回收率 | 87% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人GM-CSF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的GM-CSF与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入信号增强剂孵育,洗涤去除未结合的物质后,再次加入Streptavidin-HRP。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中GM-CSF的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
CSF2 分子靶点信息概述
- 分子名:CSF2, colony stimulating factor 2
- 别名:GM-CSF; GMCSF
- 全称:sargramostim; molgramostim; granulocyte-macrophage colony stimulating factor; colony stimulating factor 2 (granulocyte-macrophage)
CSF2 分子靶点综述
粒细胞-巨噬细胞集落刺激因子(GM-CSF)是一种调节白细胞生长的细胞因子。GM-CSF刺激干细胞产生粒细胞(中性粒细胞、嗜酸性粒细胞和嗜碱性粒细胞)及单核细胞。此外,它对造血和非造血谱系细胞也具有活性。GM-CSF用作药物来刺激白细胞的产生,从而预防化疗后引起的中性粒细胞减少症。近来,人们正在临床试验评估GM-CSF作为一种潜在的疫苗佐剂对HIV感染患者的疗效。相关研究报道一些癌症(包括肺癌和急性髓细胞白血病)患者的血清中具有较高水平的GM-CSF,同样在支气管哮喘患者外周血和鳞屑提取物中也具有较高水平的GM-CSF。
人 Human CSF2 分子靶点信息
- 分子名:CSF2, colony stimulating factor 2
- 别称:
- colony stimulating factor 2 (granulocyte-macrophage)
- colony-stimulating factor
- CSF
- GMCSF
- granulocyte macrophage-colony stimulating factor
- granulocyte-macrophage colony stimulating factor
- granulocyte-macrophage colony-stimulating factor
- MGC131935
- MGC138897
- molgramostim
- molgramostin
- sargramostim
- 基因序列:NCBI_Gene: 1437
- 蛋白序列:UniProtKB: P04141
人 Human CSF2靶点分子功能(预测)
Enables cytokine activity. Involved in several processes, including cellular response to granulocyte macrophage colony-stimulating factor stimulus; myeloid leukocyte differentiation; and positive regulation of macromolecule metabolic process. Located in extracellular space and intracellular membrane-bounded organelle. Implicated in acute myeloid leukemia; juvenile myelomonocytic leukemia; neutropenia; pulmonary alveolar proteinosis; and visceral leishmaniasis. Biomarker of COVID-19; asthma; chronic obstructive pulmonary disease; and temporal arteritis.
引用文献统计
该产品被引用的文献总数为:11
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影响因子:7.561刊物:Frontiers in Immunology发表日期:2021/4/14 - An Oncolytic Adenovirus Encoding Decorin and Granulocyte Macrophage Colony Stimulating Factor Inhibits Tumor Growth in a Colorectal Tumor Model by Targeting Pro-Tumorigenic Signals and via Immune Activation
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影响因子:5.988刊物:Frontiers in Pharmacology发表日期:2022/5/5 - Knockdown of lncRNA ANRIL suppresses the production of inflammatory cytokines and mucin 5AC in nasal epithelial cells via the miR-15a-5p/JAK2 axis
影响因子:2.1刊物:Molecular Medicine Reports发表日期:2020/12/15 - Impaired myeloid-derived suppressor cells are associated with recurrent implantation failure: A case-control study
影响因子:4.054刊物:JOURNAL OF REPRODUCTIVE IMMUNOLOGY发表日期:2021/3/29 - IDH2/R140Q mutation confers cytokine-independent proliferation of TF-1 cells by activating constitutive STAT3/5 phosphorylation
影响因子:8.2刊物:Cell Communication and Signaling发表日期:2024-02-12