EK133HS

Human IL-33 High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

说明书

在售SKU:70-EK133HS-48, 70-EK133HS-96

种属：人
样本类型：血清、血浆、细胞培养上清及其他生物学样本
检测样本体积：血清血浆：20μL；细胞培养上清：100μL
灵敏度：0.16pg/mL
检测范围：6.25-400pg/mL
回收率：92%-110%

ELISA试剂盒详细信息

商品名	人白介素33高敏酶联免疫检测试剂盒
种属	人
检测方法	双抗体夹心法
检测样本类型	血清、血浆、细胞培养上清及其他生物学样本
检测样本体积	血清血浆：20μL；细胞培养上清：100μL
灵敏度	0.16pg/mL
线性范围	6.25-400pg/mL
精密度	板内变异系数：4.0%-4.6%;板间变异系数：2.6%-4.8%
回收率	92%-110%
平均回收率	99%
板式	96孔板，可拆
保存条件	2-8℃保存。已拆开：标准品-20℃保存，其它4℃。
运输条件	2-8℃冰袋运输
检测原理	本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-33抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IL-33与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-33的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。

引用文献统计

该产品被引用的文献总数为：10

Imbalance of Th17, Treg, and helper innate lymphoid cell in the peripheral blood of patients with rheumatoid arthritis
影响因子：3.65刊物：CLINICAL RHEUMATOLOGY发表日期：2022/8/4
Effects of myeloid and plasmacytoid dendritic cells on ILC2s in patients with allergic rhinitis
影响因子：14.11刊物：JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY发表日期：2019/12/5
Soluble ST2 (sST2) as potential marker for hepatic cystic echinococcosis activity
影响因子：4.842刊物：JOURNAL OF INFECTION发表日期：2020/2/3
Altered levels of circulating CD8+CXCR5+PD-1+T follicular cytotoxic cells in primary Sjögren’s syndrome
影响因子：3.65刊物：CLINICAL RHEUMATOLOGY发表日期：2022/2/16
Predictive Values of Serum IL-33 and sST2 in Endotypes and Postoperative Recurrence of Chronic Rhinosinusitis with Nasal Polyps
影响因子：4.529刊物：MEDIATORS OF INFLAMMATION发表日期：2022/5/19
Lactate Transporter SLC16A3 (MCT4) as an Onco-Immunological Biomarker Associating Tumor Microenvironment and Immune Responses in Lung Cancer
影响因子：2.145刊物：International Journal of General Medicine发表日期：2022/11/14
Revealing the regulation of allergic asthma airway epithelial cell inflammation by STEAP4 targeting MIF through machine learning algorithms and single-cell sequencing analysis
影响因子：3.9刊物：Frontiers in Molecular Biosciences发表日期：2024-08-08
The egg ribonuclease SjCP1412 accelerates liver fibrosis caused by Schistosoma japonicum infection involving damage associated molecular patterns (DAMPs)
影响因子：2.4刊物：PARASITOLOGY发表日期：2023-12-18
Shen Qi Wan ameliorates nephritis in chronic kidney disease via AQP1 and DEFB1 regulation
影响因子：7.5刊物：BIOMEDICINE & PHARMACOTHERAPY发表日期：2023-12-18
The enrichment of Arg1+ILC2s and ILCregs facilitates the progression of endometriosis: A preliminary study
影响因子：5.6刊物：INTERNATIONAL IMMUNOPHARMACOLOGY发表日期：2023-06-09

高敏试剂盒操作常见问题

小心！你的 ELISA 试剂盒可能是假的
开学学习计划，如何收集ELISA检测样本——细胞
开学学习计划，如何选择ELISA试剂盒
开学学习计划，ELISA从新手到入门！
一文掌握ELISA实验显色判断、数据分析及标曲拟合
血清OR血浆，哪个是ELISA的菜
ELISA通关必备丨数据篇丨标准曲线不佳
ELISA通关必备丨操作篇丨常见问题及解决方案
ELISA通关必备丨操作篇丨溶解与稀释标准品
ELISA通关必备丨样本篇丨不常见样本
ELISA通关必备丨样本篇丨常见样本丨细胞
ELISA通关必备丨如何选择试剂盒

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操作步骤

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ELISA操作常见问题
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ELISA操作常见问题

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引用文献

文章目录[隐藏]

Imbalance of Th17, Treg, and helper innate lymphoid cell in the peripheral blood of patients with rheumatoid arthritis
Effects of myeloid and plasmacytoid dendritic cells on ILC2s in patients with allergic rhinitis
Soluble ST2 (sST2) as potential marker for hepatic cystic echinococcosis activity
Altered levels of circulating CD8+CXCR5+PD-1+T follicular cytotoxic cells in primary Sjögren’s syndrome
Predictive Values of Serum IL-33 and sST2 in Endotypes and Postoperative Recurrence of Chronic Rhinosinusitis with Nasal Polyps
Lactate Transporter SLC16A3 (MCT4) as an Onco-Immunological Biomarker Associating Tumor Microenvironment and Immune Responses in Lung Cancer
Revealing the regulation of allergic asthma airway epithelial cell inflammation by STEAP4 targeting MIF through machine learning algorithms and single-cell sequencing analysis
The egg ribonuclease SjCP1412 accelerates liver fibrosis caused by Schistosoma japonicum infection involving damage associated molecular patterns (DAMPs)
Shen Qi Wan ameliorates nephritis in chronic kidney disease via AQP1 and DEFB1 regulation
The enrichment of Arg1+ILC2s and ILCregs facilitates the progression of endometriosis: A preliminary study