EK212
Mouse IL-12p70 ELISA Kit检测试剂盒（酶联免疫吸附法）
¥1,600.00 – ¥2,650.00
在售SKU:70-EK212/3-48, 70-EK212/3-96, EK212
- 分子靶点：IL12
- 种属：小鼠
- 样本类型：血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积：血清、血浆样本20μL
- 灵敏度：3.58pg/mL
- 检测范围：31.25-2000pg/mL
- 回收率：89%-114%
ELISA试剂盒详细信息
| 商品名 | 小鼠IL-12p70 酶联免疫检测试剂盒 |
|---|---|
| 种属 | 小鼠 |
| 靶点 | IL12 |
| 检测方法 | 双抗体夹心法 |
| 检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
| 检测样本体积 | 血清、血浆样本20μL |
| 灵敏度 | 3.58pg/mL |
| 线性范围 | 31.25-2000pg/mL |
| 精密度 | 板内变异系数：5.2%-9.4%;板间变异系数：6.5%-7.9% |
| 回收率 | 89%-114% |
| 平均回收率 | 99% |
| 板式 | 96孔板，可拆 |
| 保存条件 | 2-8℃保存。已拆开：标准品-20℃保存，其它4℃。 |
| 运输条件 | 2-8℃冰袋运输 |
| 检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-12p70抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-12p70与固相抗体和检测抗体结合。洗涤去除未结合的物 质 后， 加 入 辣 根 过氧 化 物 酶 标 记的 链 霉 亲 和 素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-12p70的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
引用文献统计
该产品被引用的文献总数为：108
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