Human IL-9 High Sensitivity ELISA Kit检测试剂盒(酶联免疫吸附法)
¥2,000.00 – ¥3,400.00
在售SKU:70-EK109HS-48, 70-EK109HS-96, 人白细胞介素9, 白细胞介素9, 白细胞介素, 人, 白介素, 9
- 分子靶点:IL9
- 种属:人
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清血浆:20μL;细胞培养上清:100μL
- 灵敏度:0.16pg/mL
- 检测范围:0.63-40pg/mL
- 回收率:98%-116%
ELISA试剂盒详细信息
商品名 | 人白介素9高敏酶联免疫检测试剂盒 |
---|---|
种属 | 人 |
靶点 | IL9 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清血浆:20μL;细胞培养上清:100μL |
灵敏度 | 0.16pg/mL |
线性范围 | 0.63-40pg/mL |
精密度 | 板内变异系数:3.8%-4.3%;板间变异系数:3.9%-4.8% |
回收率 | 98%-116% |
平均回收率 | 102% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-9抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的IL-9与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入信号增强剂孵育,洗涤去除未结合的物质后,再次加入Streptavidin-HRP。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-9的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
IL9 分子靶点信息概述
- 分子名:IL9, interleukin 9
- 基因家族:Interleukins
- 别名:IL-9; HP40; P40
- 全称:p40 T-cell and mast cell growth factor; T-cell growth factor p40; p40 cytokine; homolog of mouse T cell and mast cell growth factor 40
IL9 分子靶点综述
白细胞介素9(IL-9)是一个分子量为14kDa的糖基化蛋白,包含10个参与二硫键连接的半胱氨酸残基。它是由T细胞特别是CD4+辅助性细胞产生的细胞因子,可调节多种造血细胞。该细胞因子刺激细胞增殖、阻止细胞凋亡。它是通过与IL-9受体结合起作用的,可激活不同的信号转导及激活(STAT)蛋白,从而参与多种生物过程。编码该细胞因子的基因已被确定为哮喘的候选基因。小鼠模型的哮喘遗传研究表明,这种细胞因子是支气管高反应发病机制的一个决定性因素。IL-9介导的自分泌环的存在暗示着一些恶性肿瘤如霍奇金病的存在。IL-9由李-斯二氏细胞、霍奇金淋巴瘤细胞和一些大型再生障碍性淋巴瘤细胞表达,而非霍奇金淋巴瘤和外周T细胞淋巴瘤不表达。
人 Human IL9 分子靶点信息
- 分子名:IL9, interleukin 9
- 别称:
- cytokine P40
- homolog of mouse T cell and mast cell growth factor 40
- HP40
- IL-9
- interleukin-9
- P40
- p40 cytokine
- p40 T-cell and mast cell growth factor
- T-cell growth factor p40
- 基因序列:NCBI_Gene: 3578
- 蛋白序列:UniProtKB: P15248
人 Human IL9靶点分子功能(预测)
Predicted to enable cytokine activity and interleukin-9 receptor binding activity. Predicted to be involved in positive regulation of interleukin-5 production. Predicted to act upstream of or within several processes, including B cell activation; regulation of cellular protein metabolic process; and regulation of receptor signaling pathway via JAK-STAT. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Implicated in asthma and respiratory syncytial virus infectious disease. Biomarker of COVID-19; asthma; cystic fibrosis; lung disease (multiple); and rhinitis.
引用文献统计
该产品被引用的文献总数为:10
- Anti−IL-12/23 p40 antibody attenuates chronic graft-versus-host disease with lupus nephritis via inhibiting Tfh cell in mice
影响因子:4.545刊物:BIOMEDICINE & PHARMACOTHERAPY发表日期:2020/6/21 - HMGB1-induced ILC2s activate dendritic cells by producing IL-9 in asthmatic mouse model
影响因子:4.078刊物:CELLULAR IMMUNOLOGY发表日期:2020/3/6 - Acupuncture inhibited airway inflammation and group 2 innate lymphoid cells in the lung in an ovalbumin-induced murine asthma model
影响因子:2.267刊物:Acupuncture in Medicine发表日期:2021/6/1 - TL1A modulates the severity of colitis by promoting Th9 differentiation and IL-9 secretion
影响因子:3.448刊物:LIFE SCIENCES发表日期:2019/6/6 - Interleukin-9 blockage reduces early hepatic granuloma formation and fibrosis during Schistosoma japonicum infection in mice
影响因子:4.147刊物:IMMUNOLOGY发表日期:2019/8/22 - The Effect of Entecavir Therapy on Immune Status in Chronic Hepatitis B Patients
影响因子:0.937刊物:Iranian Journal of Immunology发表日期:2019/3/1 - Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis
影响因子:2.548刊物:Reproductive Sciences发表日期:2018/11/19 - Effect of crosstalk between Th17 and Th9 cells on the activation of dermal vascular smooth muscle cells in systemic scleroderma and regulation of tanshinone IIA
影响因子:2.113刊物:ANAIS BRASILEIROS DE DERMATOLOGIA发表日期:2022/11/14 - Expression profile of PU.1 in CD4+T cells from patients with systemic lupus erythematosus
影响因子:3.984刊物:CLINICAL AND EXPERIMENTAL MEDICINE发表日期:2021/5/8 - Decreased frequency of circulating Th9 cells in patients with chronic hepatitis B infection
影响因子:1.521刊物:JOURNAL OF CLINICAL LABORATORY ANALYSIS发表日期:2017/5/8