Mouse IL-9 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
在售SKU:70-EK209/2-48, 70-EK209/2-96, EK209
- 分子靶点:IL9
- 种属:小鼠
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本20μL
- 灵敏度:3.59pg/mL
- 检测范围:125-8000pg/mL
- 回收率:95%-117%
ELISA试剂盒详细信息
商品名 | 小鼠白介素9酶联免疫检测试剂盒 |
---|---|
种属 | 小鼠 |
靶点 | IL9 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本20μL |
灵敏度 | 3.59pg/mL |
线性范围 | 125-8000pg/mL |
精密度 | 板内变异系数:2.5%-3.7%;板间变异系数:2.8%-4.6% |
回收率 | 95%-117% |
平均回收率 | 108% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-9抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-9与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-9的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
IL9 分子靶点信息概述
- 分子名:IL9, interleukin 9
- 基因家族:Interleukins
- 别名:IL-9; HP40; P40
- 全称:p40 T-cell and mast cell growth factor; T-cell growth factor p40; p40 cytokine; homolog of mouse T cell and mast cell growth factor 40
IL9 分子靶点综述
白细胞介素9(IL-9)是一个分子量为14kDa的糖基化蛋白,包含10个参与二硫键连接的半胱氨酸残基。它是由T细胞特别是CD4+辅助性细胞产生的细胞因子,可调节多种造血细胞。该细胞因子刺激细胞增殖、阻止细胞凋亡。它是通过与IL-9受体结合起作用的,可激活不同的信号转导及激活(STAT)蛋白,从而参与多种生物过程。编码该细胞因子的基因已被确定为哮喘的候选基因。小鼠模型的哮喘遗传研究表明,这种细胞因子是支气管高反应发病机制的一个决定性因素。IL-9介导的自分泌环的存在暗示着一些恶性肿瘤如霍奇金病的存在。IL-9由李-斯二氏细胞、霍奇金淋巴瘤细胞和一些大型再生障碍性淋巴瘤细胞表达,而非霍奇金淋巴瘤和外周T细胞淋巴瘤不表达。
小鼠 Mouse Il9 分子靶点信息
- 分子名:Il9, interleukin 9
- 别称:
- Il-9
- P40
- 基因序列:NCBI_Gene: 16198
- 蛋白序列:
小鼠 Mouse Il9 靶点分子功能(预测)
Enables cytokine activity. Involved in positive regulation of interleukin-5 production. Acts upstream of or within several processes, including B cell activation; regulation of cellular protein metabolic process; and regulation of receptor signaling pathway via JAK-STAT. Located in extracellular space. Is expressed in gut; liver; thymus; and thymus primordium. Human ortholog(s) of this gene implicated in asthma and respiratory syncytial virus infectious disease. Orthologous to human IL9 (interleukin 9).
引用文献统计
该产品被引用的文献总数为:11
- Anti−IL-12/23 p40 antibody attenuates chronic graft-versus-host disease with lupus nephritis via inhibiting Tfh cell in mice
影响因子:4.545刊物:BIOMEDICINE & PHARMACOTHERAPY发表日期:2020/6/21 - HMGB1-induced ILC2s activate dendritic cells by producing IL-9 in asthmatic mouse model
影响因子:4.078刊物:CELLULAR IMMUNOLOGY发表日期:2020/3/6 - Therapeutic Effect of Renifolin F on Airway Allergy in an Ovalbumin-Induced Asthma Mouse Model In Vivo
影响因子:4.927刊物:MOLECULES发表日期:2022/6/12 - Dynamics of Th9 cells and their potential role in immunopathogenesis of murine schistosomiasis
影响因子:3.035刊物:Parasites & Vectors发表日期:2017/6/24 - Irradiated whole‑cell vaccine suppresses hepatocellular carcinoma growth in mice via Th9 cells
影响因子:2.967刊物:Oncology Letters发表日期:2021/3/22 - Acupuncture inhibited airway inflammation and group 2 innate lymphoid cells in the lung in an ovalbumin-induced murine asthma model
影响因子:2.267刊物:Acupuncture in Medicine发表日期:2021/6/1 - TL1A modulates the severity of colitis by promoting Th9 differentiation and IL-9 secretion
影响因子:3.448刊物:LIFE SCIENCES发表日期:2019/6/6 - Interleukin-9 blockage reduces early hepatic granuloma formation and fibrosis during Schistosoma japonicum infection in mice
影响因子:4.147刊物:IMMUNOLOGY发表日期:2019/8/22 - Tumor cells inhibit the activation of ILC2s through up-regulating PD-1 expression
影响因子:2.9刊物:IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY发表日期:2024-04-24 - Baiheqingjin formula reduces inflammation in mice with asthma by inhibiting the PI3K/AKT/NF-κb signaling pathway
影响因子:5.4刊物:JOURNAL OF ETHNOPHARMACOLOGY发表日期:2023-12-09 - Effects of total alkaloids from Alstonia scholaris (L.) R. Br. on ovalbumin-induced asthma mice
影响因子:5.4刊物:JOURNAL OF ETHNOPHARMACOLOGY发表日期:2023-07-17