EK112

Human IL-12p70 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：IL12, IL-12, CLMF
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：血清血浆：10 μL；细胞培养上清：100 μL
灵敏度：0.32 pg/mL
检测范围：1.56 pg/mL - 100 pg/mL
回收率：84% - 119%

描述

文章目录[隐藏]

IL12A 分子靶点信息概述
IL12B 分子靶点信息概述
IL12B 分子靶点综述
IL12, interleukin 12 分子靶点种属信息

商品名	Human IL-12p70 ELISA Kit (人白介素12p70 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：5.5% - 7.5%；板间变异系数：3.2% - 7.5%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	血清血浆：10 μL；细胞培养上清：100 μL
灵敏度	0.32 pg/mL
检测范围	1.56 pg/mL - 100 pg/mL
回收率	84% - 119%
平均回收率	1.03
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IL-12p70单克隆抗体的96孔聚苯乙烯酶标板人IL-12p70冻干标准品 IL-12p70检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物（TMB），避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应，在450nm波长(参考波长5630nm)测定吸光度值。

Human IL-12p70 ELISA Kit (人白介素12p70 ELISA试剂盒) - 标准曲线 — 标准曲线

IL12A 分子靶点信息概述IL12B 分子靶点信息概述

IL12A 分子靶点信息概述

分子名：IL12A, interleukin 12A
基因家族：Interleukins
别名：CLMF; IL-12A; p35; NFSK
曾用名：NKSF1
全称：nterleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); natural killer cell stimulatory factor 1, 35 kD subunit; cytotoxic lymphocyte maturation factor 1, p35; interleukin 12, p35; IL-12, subunit p35; NF cell stimulatory factor chain 1; interleukin-12 alpha chain; IL35 subunit

IL12B 分子靶点信息概述

分子名：IL12B, interleukin 12B
基因家族：Immunoglobulin like domain containing; Interleukins
别名：CLMF; IL-12B; NKSF; CLMF2
曾用名：NKSF2
全称：natural killer cell stimulatory factor-2; cytotoxic lymphocyte maturation factor 2, p40; interleukin 12, p40; natural killer cell stimulatory factor, 40 kD subunit; interleukin-12 beta chain; IL12, subunit p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)

IL12B 分子靶点综述

白细胞介素12 (IL-12)由树突状细胞、巨噬细胞、中性粒细胞和人B淋巴母细胞株 (NC-37)在响应抗原刺激时自然产生。IL-12由4个а螺旋组成，是一个由2种独立基因--- IL-12A (p35)和IL-12B (p40)编码形成的异源二聚体细胞因子。蛋白合成之后可形成有生物活性的异源二聚体(p70)和同源二聚体p40。IL-12参与初始T细胞向Th1细胞分化的过程，作为T细胞刺激因子，它可刺激T细胞的生长及其功能发挥。IL-12具有抗血管生成活性，对自然杀伤细胞和T淋巴细胞活性有重要作用。同样地，IL-12与自身免疫相关。

IL12, interleukin 12 分子靶点种属信息

人 Human IL1A人 Human IL1B

人 Human IL12A 分子靶点信息

分子名：IL12A, interleukin 12A
别称：
- CLMF
- CLMF p35
- cytotoxic lymphocyte maturation factor 1, p35
- cytotoxic lymphocyte maturation factor 35 kDa subunit
- IL-12 subunit p35
- IL-12, subunit p35
- IL-12A
- IL35 subunit
- interleukin 12, p35
- interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
- interleukin-12 alpha chain
- interleukin-12 subunit alpha
- natural killer cell stimulatory factor 1, 35 kD subunit
- NF cell stimulatory factor chain 1
- NFSK
- NK cell stimulatory factor chain 1
- NKSF1
- P35
基因序列：NCBI_Gene: 3592
蛋白序列：UniProtKB: P29459

人 Human IL12A靶点分子功能（预测）

Enables interleukin-12 beta subunit binding activity; interleukin-27 binding activity; and protein heterodimerization activity. Contributes to cytokine activity and growth factor activity. Involved in several processes, including positive regulation of leukocyte mediated cytotoxicity; positive regulation of lymphocyte proliferation; and response to bacterium. Acts upstream of or within negative regulation of protein secretion. Located in extracellular space. Part of interleukin-12 complex. Implicated in hepatitis C; hepatocellular carcinoma; and primary biliary cholangitis. Biomarker of hepatitis C.

人 Human IL12B 分子靶点信息

分子名：IL12B, interleukin 12B
别称：
- CLMF
- CLMF p40
- CLMF2
- cytotoxic lymphocyte maturation factor 40 kDa subunit
- IL-12 subunit p40
- IL-12B
- IL12, subunit p40
- IMD28
- IMD29
- interleukin 12, p40
- interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2
- interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
- interleukin-12 beta chain
- interleukin-12 subunit beta
- natural killer cell stimulatory factor, 40 kD subunit
- NK cell stimulatory factor chain 2
- NKSF
- NKSF2
基因序列：NCBI_Gene: 3593
蛋白序列：UniProtKB: P29460

人 Human IL12B靶点分子功能（预测）

Enables identical protein binding activity; interleukin-12 alpha subunit binding activity; and protein heterodimerization activity. Contributes to cytokine activity; growth factor activity; and interleukin-23 receptor binding activity. Involved in several processes, including positive regulation of lymphocyte proliferation; positive regulation of peptidyl-tyrosine phosphorylation; and regulation of cytokine production. Acts upstream of or within negative regulation of protein secretion. Part of interleukin-12 complex and interleukin-23 complex. Implicated in asthma; chronic obstructive pulmonary disease; immunodeficiency 29; and salmonellosis. Biomarker of anogenital venereal wart.