Human Fas/TNFRSF6/CD95 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
在售SKU:70-EK1F01-48, 70-EK1F01-96, EK1F01
- 分子靶点:FAS
- 种属:人
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本20μL
- 灵敏度:3.15pg/mL
- 检测范围:31.25-2000pg/mL
- 回收率:95%-106%
ELISA试剂盒详细信息
商品名 | 人Fas/肿瘤坏死因子受体超家族成员6/CD95酶联免疫检测试剂盒 |
---|---|
种属 | 人 |
靶点 | FAS |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本20μL |
灵敏度 | 3.15pg/mL |
线性范围 | 31.25-2000pg/mL |
精密度 | 板内变异系数:2.0%-3.9%;板间变异系数:3.2%-5.0% |
回收率 | 95%-106% |
平均回收率 | 102% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人Fas抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的Fas与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中Fas的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
FAS 分子靶点信息概述
- 分子名:FAS, Fas cell surface death receptor
- 基因家族:CD molecules; Tumor necrosis factor receptor superfamily; Death inducing signaling complex
- 别名:CD95; APO-1
- 曾用名:FAS1; APT1; TNFRSF6
- 全称:TNF receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6; Fas (TNF receptor superfamily, member 6)
FAS 分子靶点综述
Fas,又名APO-1、CD95或TNFRSF6,是一个可转导凋亡信号的细胞表面受体。它是两种凋亡途径之一,另一种是线粒体途径。一旦结合配体,Fas就会形成死亡诱导信号复合物。相邻细胞表面的膜锚定Fas配体三聚体会导致Fas低聚化。近来的小鼠模型研究表明,Fas促进其肿瘤生长,但是人类癌症基因组学数据库表明Fas可作为肿瘤抑制物。在AOM-DSS诱导的结肠癌和MCA诱导的肉瘤小鼠模型中,结果显示Fas是肿瘤抑制物。另外,Fas还可介导肿瘤特异性细胞毒性T细胞的抗肿瘤细胞毒作用。研究报道,癌症和自身免疫性疾病患者的血清Fas水平是升高的,前者可能来源于肿瘤细胞本身。
人 Human FAS 分子靶点信息
- 分子名:FAS, Fas cell surface death receptor
- 别称:
- ALPS1A
- APO-1
- apo-1 antigen
- APO-1 cell surface antigen
- apoptosis antigen 1
- apoptosis signaling receptor FAS
- apoptosis-mediating surface antigen FAS
- APT1
- CD95
- CD95 antigen
- Delta Fas/APO-1/CD95
- Fas (TNF receptor superfamily, member 6)
- FAS 827dupA
- Fas AMA
- Fas antigen
- FAS receptor variant 9
- FAS1
- FASLG receptor
- FASTM
- mutant tumor necrosis receptor superfamily member 6
- RP11-399O19.9
- TNF receptor superfamily member 6
- TNFRSF6
- tumor necrosis factor receptor superfamily member 6
- tumor necrosis factor receptor superfamily, member 6
- 基因序列:NCBI_Gene: 355
- 蛋白序列:UniProtKB: P25445
人 Human FAS靶点分子功能(预测)
Enables calmodulin binding activity; identical protein binding activity; and kinase binding activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of cellular protein metabolic process; and regulation of signal transduction. Acts upstream of or within apoptotic process. Located in several cellular components, including cell surface; membrane raft; and nuclear body. Part of CD95 death-inducing signaling complex. Implicated in several diseases, including autoimmune disease (multiple); cystic fibrosis; hematologic cancer (multiple); pre-eclampsia (multiple); and urinary system cancer (multiple). Biomarker of several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); and neurodegenerative disease (multiple).