EK382HS

Rat TNF-α High Sensitivity ELISA Kit检测试剂盒(酶联免疫吸附法)

¥2,000.00¥3,400.00

在售SKU:70-EK382HS-48, 70-EK382HS-96

  • 分子靶点:TNF
  • 种属:大鼠
  • 样本类型:血清、血浆、细胞培养上清及其他生物学样本
  • 检测样本体积:血清、血浆样本20μL
  • 灵敏度:1.73pg/mL
  • 检测范围:7.81-500pg/mL
  • 回收率:92%-127%

ELISA试剂盒详细信息

商品名大鼠肿瘤坏死因子α高敏酶联免疫检测试剂盒
种属大鼠
靶点TNF
检测方法双抗体夹心法
检测样本类型血清、血浆、细胞培养上清及其他生物学样本
检测样本体积血清、血浆样本20μL
灵敏度1.73pg/mL
线性范围7.81-500pg/mL
精密度板内变异系数:4.2%-4.8%;板间变异系数:10.1%-5.8%
回收率92%-127%
平均回收率112%
板式96孔板,可拆
保存条件2-8℃保存。已拆开:标准品-20℃保存,其它4℃。
运输条件2-8℃冰袋运输
检测原理本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠TNF-α抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的TNF-α与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入信号增强剂孵育,洗涤去除未结合的物质后,再次加入Streptavidin-HRP。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中TNF-α的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570-630nm)测定吸光度值。

分子信息

概述大鼠 TNF, TNFA, TNFSF2 靶点信息

TNF 分子靶点信息概述

  • 分子名:TNF, tumor necrosis factor
  • 基因家族:Tumor necrosis factor superfamily
  • 别名:TNFSF2; DIF; TNF-alpha
  • 曾用名:TNFA
  • 全称:TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF 分子靶点综述

TNF-α 为单核细胞、巨噬细胞、中性粒细胞、NK 细胞和 CD4+T 细胞产生的 17 kDa 细胞因子。它在免疫应答中具有多种调节功能,还可作为潜在的热原。TNF-α对刺激物(感染因子或组织受损)产生应答后,在全身循环,激活中性粒细胞,改变血管内皮细胞的特性,调控其它组织的代谢活性,以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α 在关节组织和其它组织发生炎症的发病机制具有重要作用。

大鼠 Rat Tnf 分子靶点信息

  • 分子名:Tnf, tumor necrosis factor
  • 别称:
    • cachectin
    • LOC103694380
    • MGC124630
    • RATTNF
    • TNF-a
    • TNF-alpha
    • Tnfa
    • tumor necrosis factor (TNF superfamily, member 2)
    • tumor necrosis factor alpha
    • tumor necrosis factor ligand superfamily member 2
    • tumor necrosis factor superfamily member 2
    • tumor necrosis factor superfamily, member 2
    • tumor necrosis factor-alpha
    • tumor necrosis factor-like
  • 基因序列:NCBI_Gene: 24835
  • 蛋白序列:UniProtKB: P16599

大鼠 Rat Tnf 靶点分子功能(预测)

Enables tumor necrosis factor receptor binding activity. Involved in several processes, including positive regulation of cell communication; positive regulation of macromolecule metabolic process; and positive regulation of neuron death. Located in several cellular components, including external side of plasma membrane; extracellular space; and neuronal cell body. Used to study several diseases, including cerebrovascular disease (multiple); liver disease (multiple); peptic esophagitis; perinatal necrotizing enterocolitis; and periventricular leukomalacia. Biomarker of several diseases, including brain disease (multiple); congestive heart failure (multiple); inflammatory bowel disease (multiple); lung disease (multiple); and non-alcoholic fatty liver disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).

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