EK282

Mouse TNF-a ELISA Kit检测试剂盒(酶联免疫吸附法)

¥1,600.00¥10,800.00

在售SKU:70-EK282-48, 70-EK282-96, EK282

  • 分子靶点:TNF
  • 种属:小鼠
  • 样本类型:血清、血浆、细胞培养上清及其他生物学样本
  • 检测样本体积:血清样本20μL
  • 灵敏度:0.99pg/mL
  • 检测范围:10.94-700pg/mL
  • 回收率:81%-119%

ELISA试剂盒详细信息

商品名小鼠肿瘤坏死因子α酶联免疫检测试剂盒
种属小鼠
靶点TNF
检测方法双抗体夹心法
检测样本类型血清、血浆、细胞培养上清及其他生物学样本
检测样本体积血清样本20μL
灵敏度0.99pg/mL
线性范围10.94-700pg/mL
精密度板内变异系数:3.1%-4.5%;板间变异系数:3.8%-5.1%
回收率81%-119%
平均回收率101%
板式96孔板,可拆
保存条件2-8℃保存。已拆开:标准品-20℃保存,其它4℃。
运输条件2-8℃冰袋运输
检测原理本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本,经过孵育,样本中存在的待测物质与捕获抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的检测抗体孵育。洗涤后,加入显色底物(TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。

分子信息

概述小鼠 TNF, TNFA, TNFSF2 靶点信息

TNF 分子靶点信息概述

  • 分子名:TNF, tumor necrosis factor
  • 基因家族:Tumor necrosis factor superfamily
  • 别名:TNFSF2; DIF; TNF-alpha
  • 曾用名:TNFA
  • 全称:TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF 分子靶点综述

TNF-α 为单核细胞、巨噬细胞、中性粒细胞、NK 细胞和 CD4+T 细胞产生的 17 kDa 细胞因子。它在免疫应答中具有多种调节功能,还可作为潜在的热原。TNF-α对刺激物(感染因子或组织受损)产生应答后,在全身循环,激活中性粒细胞,改变血管内皮细胞的特性,调控其它组织的代谢活性,以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α 在关节组织和其它组织发生炎症的发病机制具有重要作用。

小鼠 Mouse Tnf 分子靶点信息

小鼠 Mouse Tnf 靶点分子功能(预测)

Enables cytokine activity; protease binding activity; and tumor necrosis factor receptor binding activity. Involved in several processes, including endothelial cell apoptotic process; positive regulation of cell communication; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including apoptotic signaling pathway; positive regulation of intracellular signal transduction; and regulation of gene expression. Located in several cellular components, including external side of plasma membrane; phagocytic cup; and recycling endosome. Is expressed in several structures, including alimentary system; brain; integumental system; lung; and reproductive system. Used to study several diseases, including autoimmune disease (multiple); congestive heart failure; heart valve disease (multiple); idiopathic pulmonary fibrosis; and spondyloarthropathy. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).

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