EK282

Mouse TNF-a ELISA Kit检测试剂盒(酶联免疫吸附法)

¥1,600.00¥2,650.00

  • 分子靶点:TNF, TNFA, TNFSF2
  • 种属:小鼠 (Mouse)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:20 μL
  • 灵敏度:0.99 pg/mL
  • 检测范围:10.938 pg/mL - 700 pg/mL
  • 回收率:81% - 119%

在售SKU:70-EK282-48, 70-EK282-96


描述

背景介绍 肿瘤坏死因子α(TNF-α)也称为恶病质素和TNFSF1A,是一种脂肪因子,参与全身的炎症,同时也是刺激急性期反应的细胞因子之一。它主要由活化的巨噬细胞产生,也可由其它类型的细胞分泌,如CD4+淋巴细胞、NK细胞、中性粒细胞、肥大细胞、嗜酸性粒细胞和神经元等。它在免疫应答中具有多种调节功能,还可作为潜在的热原。TNF-α对刺激物(感染因子或组织受损)产生应答后,在全身循环,激活中性粒细胞,改变血管内皮细胞的特性,调控其它组织的代谢活性,以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α在关节组织和其它组织发生炎症的发病机制具有重要作用。最近,越来越多的信息表明TNF-α也参与了AIDS的发病机制。TNF-α的测定对于移植研究也非常有用。
检测原理 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠TNF-α抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的TNF-α与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中TNF-α的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
组分 包被抗TNF-α单克隆抗体的96孔聚苯乙烯酶标板
小鼠TNF-α冻干标准品
TNF-α检测抗体
标准品稀释液
检测缓冲液(10×)
底物(TMB)
终止液
洗液(20×)
封板膜
分析类型 夹心ELISA
样本类型 血清、血浆,细胞培养上清及其他生物学样本
板式 96孔板,可拆
保存 试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。
运输条件 4℃蓝冰运输
样本体积 10 μl
灵敏度 0.99 pg/ml
标曲图
标准曲线范围 10.938 - 700 pg/ml
回收率范围 81 %-119 %
平均回收率 1.01
板间变异系数 3.8 % - 5.1 %
板内变异系数 3.1 % - 4.5 %

分子信息

概述小鼠 TNF, TNFA, TNFSF2 靶点信息

TNF 分子靶点信息概述

  • 分子名:TNF, tumor necrosis factor
  • 基因家族:Tumor necrosis factor superfamily
  • 别名:TNFSF2; DIF; TNF-alpha
  • 曾用名:TNFA
  • 全称:TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF 分子靶点综述

TNF-α 为单核细胞、巨噬细胞、中性粒细胞、NK 细胞和 CD4+T 细胞产生的 17 kDa 细胞因子。它在免疫应答中具有多种调节功能,还可作为潜在的热原。TNF-α对刺激物(感染因子或组织受损)产生应答后,在全身循环,激活中性粒细胞,改变血管内皮细胞的特性,调控其它组织的代谢活性,以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α 在关节组织和其它组织发生炎症的发病机制具有重要作用。

小鼠 Mouse Tnf 分子靶点信息

小鼠 Mouse Tnf 靶点分子功能(预测)

Enables cytokine activity; protease binding activity; and tumor necrosis factor receptor binding activity. Involved in several processes, including endothelial cell apoptotic process; positive regulation of cell communication; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including apoptotic signaling pathway; positive regulation of intracellular signal transduction; and regulation of gene expression. Located in several cellular components, including external side of plasma membrane; phagocytic cup; and recycling endosome. Is expressed in several structures, including alimentary system; brain; integumental system; lung; and reproductive system. Used to study several diseases, including autoimmune disease (multiple); congestive heart failure; heart valve disease (multiple); idiopathic pulmonary fibrosis; and spondyloarthropathy. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).

引用文献


  1. RAW 264.7 人肺癌细胞 A549 支气管肺泡灌洗液 BALF 细胞培养上清

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    The Immunoenhancement Effects of Polyethylenimine-Modified Chinese Yam Polysaccharide-Encapsulated Plga Nanoparticles as an Adjuvant 

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    Intravenous Injection of the Oncolytic Virus M1 Awakens Antitumor T Cells and Overcomes Resistance to Checkpoint Blockade 

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  8. RAW 264.7 细胞培养上清

    Immunomodulatory Effects of Fucosylated Chondroitin Sulfate from Stichopus Chloronotus on Raw 264.7 Cells 

  9. 组织匀浆 脾脏 血清

    Administration Routes of Polyethylenimine-Coated Plga Nanoparticles Encapsulating Angelica Sinensis Polysaccharide Vaccine Delivery System Affect Immune Responses 

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    Polylysine-Bilirubin Conjugates Maintain Functional Islets and Promote M2 Macrophage Polarization 

  11. 组织匀浆 结肠

    Genipin-Crosslinked Human Serum Albumin Coating Using a Tannic Acid Layer for Enhanced Oral Administration of Curcumin in the Treatment of Ulcerative Colitis 

  12. 血清

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  13. 支气管肺泡灌洗液 BALF 细胞培养上清 血清

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  14. 血清

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  15. 组织匀浆 肿瘤

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  16. 皮肤 皮肤损伤组织 组织匀浆 血清

    Potential shared therapeutic and hepatotoxic mechanisms of Tripterygium wilfordii polyglycosides treating three kinds of autoimmune skin diseases by regulating IL-17 signaling pathway and Th17 cell differentiation 

  17. 组织匀浆 脑脊液 脾脏 血清

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    Molecular Mechanisms Underlying Macrophage Immunomodulatory Activity of Rubus Chingii Hu Polysaccharides 

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    Entrapment of Macrophage-Target Nanoparticles by Yeast Microparticles for Rhein Delivery in Ulcerative Colitis Treatment 

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    Living Bacterial Hydrogels for Accelerated Infected Wound Healing 

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