Mouse CXCL10/IP-10 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
在售SKU:70-EK268/2-48, 70-EK268/2-96, EK268
- 分子靶点:CXCL10
- 种属:小鼠
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清血浆:50μL;细胞培养上清:100μL
- 灵敏度:19.03pg/mL
- 检测范围:312.5-20000pg/mL
- 回收率:71%-122%
ELISA试剂盒详细信息
商品名 | 小鼠趋化因子CXC配体10/γ干扰素诱导蛋白10 酶联免疫检测试剂盒 |
---|---|
种属 | 小鼠 |
靶点 | CXCL10 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清血浆:50μL;细胞培养上清:100μL |
灵敏度 | 19.03pg/mL |
线性范围 | 312.5-20000pg/mL |
精密度 | 板内变异系数:2.2%-5.3%;板间变异系数:1.3%-3.8% |
回收率 | 71%-122% |
平均回收率 | 95% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IP-10抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IP-10与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IP-10的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
CXCL10 分子靶点信息概述
- 分子名:CXCL10, C-X-C motif chemokine ligand 10
- 基因家族:Chemokine ligands
- 别名:IFI10; IP-10; crg-2; mob-1; C7; gIP-10
- 曾用名:INP10; SCYB10
- 全称:small inducible cytokine subfamily B (Cys-X-Cys), member 10; chemokine (C-X-C motif) ligand 10
CXCL10 分子靶点综述
趋化因子配体10(CXCL10)又称为γ-干扰素诱导蛋白10(IP-10)或微小诱导细胞因子B10,是属于CXC趋化因子家族的小分子量细胞因子。多种类型的细胞对IFN-γ产生应答后可分泌IP-10,包括单核细胞、内皮细胞和成纤维细胞等。IP-10具有多种功能,如对单核/巨噬细胞、T细胞、NK细胞和树突细胞的趋化作用,促进T细胞黏附于内皮细胞,抗肿瘤活性和抑制骨髓集落形成和血管生成。
小鼠 Mouse Cxcl10 分子靶点信息
- 分子名:Cxcl10, chemokine (C-X-C motif) ligand 10
- 别称:
- C7
- CRG-2
- gIP-10
- Ifi10
- INP10
- interferon activated gene 10
- IP-10
- IP10
- mob-1
- Scyb10
- small inducible cytokine B subfamily (Cys-X-Cys), member 10
- 基因序列:NCBI_Gene: 15945
- 蛋白序列:
小鼠 Mouse Cxcl10 靶点分子功能(预测)
Predicted to enable chemoattractant activity; chemokine receptor binding activity; and heparin binding activity. Acts upstream of or within several processes, including defense response to virus; negative regulation of myoblast differentiation; and negative regulation of myoblast fusion. Located in external side of plasma membrane and extracellular space. Is expressed in several structures, including alimentary system; axial skeleton; hemolymphoid system; and liver. Human ortholog(s) of this gene implicated in hepatitis B; middle cerebral artery infarction; and type 1 diabetes mellitus. Orthologous to human CXCL10 (C-X-C motif chemokine ligand 10).
引用文献统计
该产品被引用的文献总数为:40
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