Human VEGF ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥10,800.00
在售SKU:70-EK183-48, 70-EK183-96, EK183
- 分子靶点:VEGFA
- 种属:人
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本50μL
- 灵敏度:0.95pg/mL
- 检测范围:31.25-2000pg/mL
- 回收率:77%-119%
ELISA试剂盒详细信息
商品名 | 人血管内皮生长因子酶联免疫检测试剂盒 |
---|---|
种属 | 人 |
靶点 | VEGFA |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本50μL |
灵敏度 | 0.95pg/mL |
线性范围 | 31.25-2000pg/mL |
精密度 | 板内变异系数:3.3%-4.5%;板间变异系数:4.2%-6.8 |
回收率 | 77%-119% |
平均回收率 | 100% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人VEGF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的VEGF与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中VEGF的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
VEGFA 分子靶点信息概述
- 分子名:VEGFA, vascular endothelial growth factor A
- 基因家族:VEGF family
- 别名:VEGF-A; VPF
- 曾用名:VEGF
- 全称:vascular endothelial growth factor
VEGFA 分子靶点综述
血管内皮生长因子(VEGF)是生长因子的亚家族,具体来说,属于胱氨酸结生长因子中的血小板衍生生长因子(PDGF)家族。它们是重要的信号转导蛋白,参与血管发生(胚胎循环系统从头形成)和血管生成(从已存在的脉管系统生长形成血管)。VEGF可刺激血管发生和血管生成。当血液循环不足时,可参与回复组织供氧系统。VEGF最主要的作用是血管生成。在胚胎形成期,VEGF调控内皮细胞的增殖、迁移和存活,从而调控血管密度和大小,但无法决定血管的类型。在支气管哮喘和糖尿病患者的血清中含有高浓度VEGF。当VEGF过表达时会引起疾病。供血不足时,实体肿瘤的大小受限;而表达VEGF的肿瘤则会不断生长并发生转移。VEGF过表达会导致眼睛视网膜和身体其他部分的血管性疾病。贝伐珠单抗和雷珠单抗可抑制VEGF,并控制或减缓疾病进程。
人 Human VEGFA 分子靶点信息
- 分子名:VEGFA, vascular endothelial growth factor A
- 别称:
- MGC70609
- MVCD1
- vascular endothelial growth factor
- vascular endothelial growth factor A121
- vascular endothelial growth factor A165
- vascular permeability factor
- VEGF
- VEGF-A
- VPF
- 基因序列:NCBI_Gene: 7422
- 蛋白序列:UniProtKB: P15692
人 Human VEGFA靶点分子功能(预测)
Enables several functions, including fibronectin binding activity; heparin binding activity; and signaling receptor binding activity. Involved in several processes, including positive regulation of cell migration; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including branching involved in blood vessel morphogenesis; positive regulation of endothelial cell proliferation; and regulation of nitric oxide mediated signal transduction. Located in several cellular components, including adherens junction; cell surface; and secretory granule. Implicated in several diseases, including autoimmune disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); hypertension (multiple); and reproductive organ cancer (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); inflammatory bowel disease (multiple); and respiratory system cancer (multiple).
引用文献统计
该产品被引用的文献总数为:96
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