EK1157

Human Pentraxin 3/TSG-14 ELISA Kit检测试剂盒(酶联免疫吸附法)

¥1,600.00¥2,650.00

在售SKU:70-EK1157-48, 70-EK1157-96, EK1157

  • 分子靶点:PTX3
  • 种属:
  • 样本类型:血清、血浆、细胞培养上清及其他生物学样本
  • 检测样本体积:血清、血浆样本10μL
  • 灵敏度:0.56pg/mL
  • 检测范围:31.25-2000pg/mL
  • 回收率:75%-117%

96T

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ELISA试剂盒详细信息

商品名人穿透素 3/肿瘤坏死因子诱导基因 14酶联免疫检测试剂盒
种属
靶点PTX3
检测方法双抗体夹心法
检测样本类型血清、血浆、细胞培养上清及其他生物学样本
检测样本体积血清、血浆样本10μL
灵敏度0.56pg/mL
线性范围31.25-2000pg/mL
精密度板内变异系数:2.9%-6.3%;板间变异系数:2.9%-3.8%
回收率75%-117%
平均回收率93%
板式96孔板,可拆
保存条件2-8℃保存。已拆开:标准品-20℃保存,其它4℃。
运输条件2-8℃冰袋运输
检测原理本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人Pentraxin3抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的Pentraxin3与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中Pentraxin3的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。

分子信息

概述人 PTX3, TSG-14, TNFAIP5 靶点信息

PTX3 分子靶点信息概述

  • 分子名:PTX3, pentraxin 3
  • 基因家族:Long pentraxins
  • 别名:TSG-14
  • 曾用名:TNFAIP5
  • 全称:long pentraxin 3; pentaxin-related gene, rapidly induced by IL-1 beta; tumor necrosis factor, alpha-induced protein 5; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin 3, long

PTX3 分子靶点综述

穿透素3(Pentraxin3)也称为肿瘤坏死因子诱导基因14(TSG-14),是穿透素超家族成员,具有环状多聚体结构。多种类型的细胞,尤其是单核吞噬细胞、树突细胞、成纤维细胞和内皮细胞,对初次炎症信号(如toll样受体、TNF-α、IL-1β)产生应答后,可快速产生并释放Pentraxin3。Pentraxin3是急性期应答蛋白,其在正常情况下含量较低(在小鼠中约25ng/ml,在人类中小于2ng/ml),而在内毒素性休克、败血症和其它炎症和感染条件下,急剧升高(诱导后6-8小时达到峰值),含量可达200-800ng/ml,与疾病的严重程度相关。在这些情况下,Pentraxin3是先天免疫和炎症反应局部活化的快速判断标志。研究显示Pentraxin3具有双重作用:保护机体抵抗病原体和调控自身免疫。

人 Human PTX3 分子靶点信息

  • 分子名:PTX3, pentraxin 3
  • 别称:
    • long pentraxin 3
    • pentaxin-related gene, rapidly induced by il-1 beta
    • pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor, alpha-induced protein 5
    • pentaxin-related protein PTX3
    • pentraxin 3, long
    • pentraxin-3
    • pentraxin-related gene, rapidly induced by IL-1 beta
    • pentraxin-related protein PTX3
    • TNF alpha-induced protein 5
    • TNFAIP5
    • TSG-14
    • tumor necrosis factor alpha-induced protein 5
    • tumor necrosis factor-inducible gene 14 protein
    • tumor necrosis factor-inducible protein TSG-14
    • tumor necrosis factor, alpha-induced protein 5
  • 基因序列:NCBI_Gene: 5806
  • 蛋白序列:UniProtKB: P26022

人 Human PTX3靶点分子功能(预测)

Enables complement component C1q complex binding activity; identical protein binding activity; and virion binding activity. Involved in innate immune response; modulation by host of viral process; and negative regulation of viral entry into host cell. Located in extracellular space. Implicated in cystic fibrosis and invasive aspergillosis. Biomarker of hepatitis C; invasive aspergillosis; pelvic inflammatory disease; pulmonary aspergilloma; and toxic shock syndrome.