EK981

Human/Mouse TGF-β1 ELISA Kit 检测试剂盒(酶联免疫吸附法)

¥1,600.00¥10,800.00

在售SKU:70-EK981-48, 70-EK981-96, EK981

  • 分子靶点:TGFB1
  • 种属:人、小鼠
  • 样本类型:血清、血浆、细胞培养上清及其他生物学样本
  • 检测样本体积:血清血浆40 μL;细胞上清100μL
  • 灵敏度:3.36pg/mL
  • 检测范围:31.25pg/mL-2000pg/mL
  • 回收率:83%-118%

ELISA试剂盒详细信息

商品名人/小鼠TGF-β1 酶联免疫检测试剂盒
种属人、小鼠
靶点TGFB1
检测方法双抗体夹心法
检测样本类型血清、血浆、细胞培养上清及其他生物学样本
检测样本体积血清血浆40 μL;细胞上清100μL
灵敏度3.36pg/mL
线性范围31.25pg/mL-2000pg/mL
精密度板内变异系数:2.0% - 5.3%;板间变异系数:4.1%-6.0%
回收率83%-118%
平均回收率99%
板式96孔板,可拆
保存条件2-8℃保存。已拆开:标准品-20℃保存,其它4℃。
运输条件2-8℃冰袋运输
检测原理本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物(TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。

分子信息

概述

TGFB1 分子靶点信息概述

  • 分子名:TGFB1, transforming growth factor beta 1
  • 基因家族:Transforming growth factor beta family
  • 别名:CED; TGFbeta
  • 曾用名:TGFB; DPD1
  • 全称:Camurati-Engelmann disease; prepro-transforming growth factor beta-1; Diaphyseal dysplasia 1, progressive; transforming growth factor, beta 1

TGFB1 分子靶点综述

转化生长因子β1 (TGF-β1)是转化生长因子β超家族的多肽成员,具有多种细胞学功能,包括调控细胞生长、增殖、分化和凋亡。TGF-β与TGFA协同诱导转化。它也能作为负向自分泌生长因子。TGF-β活化和信号传导的失调可能会引起凋亡。很多细胞可合成TGF-β,且几乎都有特异性的受体。TGF-β1、TGF-β2和TGF-β3都通过相同的受体信号通路发挥功能。TGF-β1对调控免疫系统具有重要作用,对不同类型的细胞或不同发育阶段的细胞具有不同的活性。大部分免疫细胞 (或白细胞) 分泌TGF-β1。TGF-β1与癌症、自身免疫疾病、肝脏疾病、肾脏疾病、糖尿病、心血管疾病、哮喘、慢性阻塞性肺疾病 (COPD)和囊肿性纤维化 (CF) 等相关。

人 Human TGFB1 分子靶点信息

  • 分子名:TGFB1, transforming growth factor beta 1
  • 别称:
    • CED
    • DPD1
    • IBDIMDE
    • LAP
    • latency-associated peptide
    • prepro-transforming growth factor beta-1
    • TGF-beta 1 protein
    • TGF-beta-1
    • TGF-beta1
    • TGFB
    • TGFbeta
    • transforming growth factor beta-1
    • transforming growth factor beta-1 proprotein
    • transforming growth factor beta1
  • 基因序列:NCBI_Gene: 7040
  • 蛋白序列:UniProtKB: P01137

人 Human TGFB1靶点分子功能(预测)

Enables several functions, including antigen binding activity; identical protein binding activity; and transforming growth factor beta receptor binding activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Acts upstream of or within epithelial to mesenchymal transition; negative regulation of gene expression; and positive regulation of isotype switching to IgA isotypes. Located in several cellular components, including cell surface; extracellular space; and nucleus. Colocalizes with microvillus. Implicated in several diseases, including Behcet's disease; Camurati-Engelmann disease; aphthous stomatitis; artery disease (multiple); and lung disease (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); carcinoma (multiple); hematologic cancer (multiple); and lung disease (multiple).

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