Human MMP-3 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
在售SKU:70-EK1M03-48, 70-EK1M03-96, EK1M03
- 分子靶点:MMP3
- 种属:人
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本10μL
- 灵敏度:0.30pg/mL
- 检测范围:31.25-2000pg/mL
- 回收率:95%-105%
ELISA试剂盒详细信息
商品名 | 人基质金属蛋白酶 3酶联免疫检测试剂盒 |
---|---|
种属 | 人 |
靶点 | MMP3 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本10μL |
灵敏度 | 0.30pg/mL |
线性范围 | 31.25-2000pg/mL |
精密度 | 板内变异系数:4.0%-4.9%;板间变异系数:4.2%-9.0% |
回收率 | 95%-105% |
平均回收率 | 101% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人MMP-3抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的MMP-3与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中MMP-3的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
MMP3 分子靶点信息概述
- 分子名:MMP3, matrix metallopeptidase 3
- 基因家族:M10 matrix metallopeptidases
- 曾用名:STMY1; STMY
- 全称:matrix metalloproteinase 3 (stromelysin 1, progelatinase); stromelysin 1
MMP3 分子靶点综述
基质金属蛋白酶3(MMP-3),又名基质分解素-1,在人类中由MMP3基因编码。MMP3的表达主要在转录水平上调控,转录水平上的基因启动子对多种刺激物包括生长因子、细胞因子、促癌剂和原癌基因产物作出应答。MMP-3酶降解II、III、IV、IX和X型胶原蛋白、蛋白聚糖、纤维连接蛋白、层粘连蛋白和弹性蛋白。另外,MMP-3还可激活其它MMPs如MMP-1、MMP-7和MMP-9,因此MMP-3在结缔组织重塑中起关键作用。该酶参与伤口修复、动脉粥样硬化进展和肿瘤启动。MMP-3野生型和基因敲除的小鼠研究表明,MMP-3增加了外伤性损伤后的血脑屏障通透性。细胞核MMP-3可作为转录因子和蛋白酶发挥作用。
人 Human MMP3 分子靶点信息
- 分子名:MMP3, matrix metallopeptidase 3
- 别称:
- CHDS6
- matrix metallopeptidase 3 (stromelysin 1, progelatinase)
- matrix metalloproteinase 3 (stromelysin 1, progelatinase)
- matrix metalloproteinase-3
- MGC126102
- MGC126103
- MGC126104
- MMP-3
- proteoglycanase
- SL-1
- STMY
- STMY1
- STR1
- stromelysin-1
- transin-1
- 基因序列:NCBI_Gene: 4314
- 蛋白序列:UniProtKB: P08254
人 Human MMP3靶点分子功能(预测)
Enables endopeptidase activity and metallopeptidase activity. Involved in several processes, including cellular response to UV-A; negative regulation of hydrogen peroxide metabolic process; and positive regulation of protein-containing complex assembly. Predicted to be located in cytosol and mitochondrion. Implicated in several diseases, including artery disease (multiple); carcinoma (multiple); end stage renal disease; osteoarthritis; and von Hippel-Lindau disease. Biomarker of several diseases, including Takayasu's arteritis; arthritis (multiple); autoimmune disease (multiple); conjunctivochalasis; and lung disease (multiple).
引用文献统计
该产品被引用的文献总数为:13
- Role of ROR2 in promoting gastric cancer metastasis by enhancing c-JUN-mediated MMP3 transcription
影响因子:3.616刊物:Annals of Translational Medicine发表日期:2022/10/1 - Elevated microRNA‑145‑5p increases matrix metalloproteinase‑9 by activating the nuclear factor‑κB pathway in rheumatoid arthritis
影响因子:1.851刊物:Molecular Medicine Reports发表日期:2019/7/15 - Follistatin-like protein 1 induction of matrix metalloproteinase 1, 3 and 13 gene expression in rheumatoid arthritis synoviocytes requires MAPK, JAK/STAT3 and NF-κB pathways
影响因子:3.923刊物:JOURNAL OF CELLULAR PHYSIOLOGY发表日期:2018/6/22 - Comparison of the therapeutic effects of human umbilical cord blood-derived mesenchymal stem cells and adipose-derived stem cells on erectile dysfunction in a rat model of bilateral cavernous nerve injury.
影响因子:6.064刊物:Frontiers in Bioengineering and Biotechnology发表日期:2022/10/7 - Aconiti Lateralis Radix Praeparata lipid-soluble alkaloids alleviates IL-1β-induced inflammation of human fibroblast-like synoviocytes in rheumatoid arthritis by inhibiting NF-κB and MAPKs signaling pathways and inducing apoptosis
影响因子:3.926刊物:CYTOKINE发表日期:2022/1/29 - Structural characterization and anti-photoaging activity of a polysaccharide from Sargassum fusiforme
影响因子:7.425刊物:FOOD RESEARCH INTERNATIONAL发表日期:2022/4/20 - The isolation, structure characterizations and anti-photoaging activities of sulfated polysaccharides isolated from Sargassum fusiforme
影响因子:5.2刊物:Chemical and Biological Technologies in Agriculture发表日期:2024-05-06 - The Potential of Bioactive Fish Collagen Oligopeptides against Hydrogen Peroxide-Induced NIH/3T3 and HUVEC Damage: The Involvement of the Mitochondria
影响因子:4.8刊物:Nutrients发表日期:2024-03-29 - Beneficial Effects of Small-Molecule Oligopeptides Isolated from Panax Ginseng C. A. Meyer on Cellular Fates in Oxidative Stress-Induced Damaged Human Umbilical Vein Endothelial Cells and PC-12
影响因子:4.9刊物:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES发表日期:2024-03-02 - Sulfasalazine promotes ferroptosis through AKT-ERK1/2 and P53-SLC7A11 in rheumatoid arthritis
影响因子:4.6刊物:INFLAMMOPHARMACOLOGY发表日期:2024-02-26 - Knockdown of Galectin-9 alleviates rheumatoid arthritis through suppressing TNF-α-induced activation of fibroblast-like synoviocytes
影响因子:5.8刊物:BIOCHEMICAL PHARMACOLOGY发表日期:2023-12-21 - Maslinic acid attenuates UVB-induced oxidative damage in HFF-1 cells
影响因子:2.3刊物:Journal of Cosmetic Dermatology发表日期:2023-03-21 - The effects of alginate lyase treatment on structural characteristics and anti-photoaging activity of alginate from Sargassum fusiforme
影响因子:9.9刊物:Food Frontiers发表日期:2023-02-24