Mouse IL-17A High Sensitivity ELISA Kit检测试剂盒(酶联免疫吸附法)
¥2,000.00 – ¥3,400.00
在售SKU:70-EK217HS-48, 70-EK217HS-96
- 分子靶点:IL17A
- 种属:小鼠
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本20μL
- 灵敏度:0.18pg/mL
- 检测范围:1.56-100pg/mL
- 回收率:82%-88%
ELISA试剂盒详细信息
商品名 | 小鼠白介素17A高敏酶联免疫检测试剂盒 |
---|---|
种属 | 小鼠 |
靶点 | IL17A |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本20μL |
灵敏度 | 0.18pg/mL |
线性范围 | 1.56-100pg/mL |
精密度 | 板内变异系数:4.1%-4.9%;板间变异系数:4.8%-9.7% |
回收率 | 82%-88% |
平均回收率 | 85% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-17A抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的IL-17A与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入信号增强剂孵育,洗涤去除未结合的物质后,再次加入Streptavidin-HRP。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-17A的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
IL17A 分子靶点信息概述
- 分子名:IL17A, interleukin 17A
- 基因家族:Interleukins
- 别名:IL-17A; IL-17
- 曾用名:CTLA8; IL17
- 全称:cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
IL17A 分子靶点综述
IL-17主要由Th17型细胞产生,是目前已发现的30余种白细胞介素中的一员,按序号排在第17位。该家族包括6个成员配体(IL-17A ~ IL-17F)和5个受体(IL-17RA ~ IL-17RD和SEF)。IL-17是一种主要由活化的T细胞产生的致炎细胞因子,可以促进T细胞的激活和刺激上皮细胞、内皮细胞、成纤维细胞产生多种细胞因子,从而导致炎症的产生。
小鼠 Mouse Il17a 分子靶点信息
- 分子名:Il17a, interleukin 17A
- 别称:
- Ctla-8
- Ctla8
- cytotoxic T lymphocyte-associated protein 8
- IL-17A
- Il17
- interleukin 17
- 基因序列:NCBI_Gene: 16171
- 蛋白序列:
小鼠 Mouse Il17a 靶点分子功能(预测)
Enables protein heterodimerization activity and protein homodimerization activity. Involved in several processes, including defense response to other organism; intestinal epithelial structure maintenance; and positive regulation of gene expression. Acts upstream of or within cellular response to interleukin-1; positive regulation of cytokine production involved in inflammatory response; and positive regulation of transcription by RNA polymerase II. Located in external side of plasma membrane and extracellular space. Human ortholog(s) of this gene implicated in Mycobacterium avium complex disease; autoimmune disease (multiple); bronchial disease (multiple); eye disease (multiple); and rhinitis (multiple). Orthologous to human IL17A (interleukin 17A).
引用文献统计
该产品被引用的文献总数为:97
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