EK201B

Mouse IL-1β ELISA Kit检测试剂盒(酶联免疫吸附法)

¥1,600.00¥10,800.00

  • 分子靶点:IL1B, IL1-β, IL1F2
  • 种属:小鼠 (Mouse)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:血清血浆:10 μL;细胞培养上清:100 μL
  • 灵敏度:1.45 pg/mL
  • 检测范围:23.44 pg/mL - 1500 pg/mL
  • 回收率:83% - 109%

在售SKU:70-EK201B/3-48, 70-EK201B/3-96


描述

商品名

Mouse IL-1β ELISA Kit (小鼠白介素1β ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.8% - 5.6%;板间变异系数:4.2% - 8.4%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

血清血浆:10 μL;细胞培养上清:100 μL

灵敏度

1.45 pg/mL

检测范围

23.44 pg/mL - 1500 pg/mL

回收率

83% - 109%

平均回收率

0.97

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗 IL-1β单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠 IL-1β冻干标准品
  • IL-1β检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-1β抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-1β与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-1β的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-1β ELISA Kit (小鼠白介素1β ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 IL1B, IL1-β, IL1F2 靶点信息

IL1B 分子靶点信息概述

  • 分子名:IL1B, interleukin 1 beta
  • 基因家族:Interleukins
  • 别名:IL1F2; IL-1B; IL1-BETA

IL1B 分子靶点综述

白细胞介素1(IL-1)是17kDa的胞外多肽,分为IL-1α和IL-1β两种蛋白。IL-1β是活化的巨噬细胞分泌的蛋白前体,通过caspase1(CASP1/ICE)水解产生的活化形式。该细胞因子是炎症反应的重要介导者,参与许多细胞活性,包括细胞增殖、分化和凋亡。IL-1是一类重要的蛋白家族,它们由生物活化的单核细胞所衍生,参与炎症反应和免疫应答。IL-1β表达升高会产生各种不同的自身炎症综合征,最显著的是单基因条件称为CAPS,是由于触发IL-1β加工的炎性体受体NLRP3发生突变而引起的。本试剂盒用来检测人IL-1β的成熟形式和未裂解的前体形式。

小鼠 Mouse Il1b 分子靶点信息

小鼠 Mouse Il1b 靶点分子功能(预测)

Enables cytokine activity. Involved in several processes, including negative regulation of cell communication; regulation of defense response; and regulation of lipid metabolic process. Acts upstream of or within several processes, including ectopic germ cell programmed cell death; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Located in extracellular space and secretory granule. Is expressed in several structures, including dorsal aorta; early conceptus; hemolymphoid system; integument; and reproductive system. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Orthologous to human IL1B (interleukin 1 beta).

操作步骤


下载本产品说明书

ELISA操作常见问题

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引用文献


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  26. Arctigenin Protects against Liver Injury from Acute Hepatitis by Suppressing Immune Cells in Mice 

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  30. RAW 264.7

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  31. Alcl(3) Inhibits Lps-Induced Nlrp3 Inflammasome Activation and Il-1β Production through Suppressing Nf-Κb Signaling Pathway in Murine Peritoneal Macrophages 

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  33. Spontaneous Hypothermia Ameliorated Inflammation and Neurologic Deficit in Rat Cardiac Arrest Models Following Resuscitation 

  34. Loss of Nlrp3 Function Alleviates Murine Hepatic Graft-Versus-Host Disease 

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Mouse IL-1β ELISA Kit检测试剂盒(酶联免疫吸附法) 有 1 个评价

  1. 周X

    能不能用于正常C57小鼠血清检测

    • 小科

      本产品可以用来检测小鼠种属的血清样本,对小鼠品系没有特殊要求,此外还可以检测小鼠血浆、细胞上清样本,但需要注意的是正常小鼠血清中 IL-1β蛋白的表达量较低,说明书中的样本上样量只是参考,实际样本间表达有差异,建议先选几个样本做个高中低的浓度梯度,摸索一下您的样本合适的上样量。根据个人经验,一般像 IL-1β这种在正常样本中表达量较低的指标选择高敏试剂盒【EK201BHS】的话,样本的可测率相对而言会更高,但也是需要先做预实验摸索样本合适的上样量的。

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