EK301B

Rat IL-1β ELISA Kit检测试剂盒(酶联免疫吸附法)

¥1,600.00¥10,800.00

  • 分子靶点:IL1B, IL1-β, IL1F2
  • 种属:大鼠 (Rat)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:20 μL
  • 灵敏度:1.42 pg/mL
  • 检测范围:31.25 pg/mL - 2000 pg/mL
  • 回收率:84% - 110%

在售SKU:70-EK301B/3-48, 70-EK301B/3-96,70-EK301B/4-48, 70-EK301B/4-96


描述

商品名

Rat IL-1β ELISA Kit (大鼠白细胞介素1β ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:5.0% - 5.1%;板间变异系数:5.5% - 6.7%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

20 μL

灵敏度

1.42 pg/mL

检测范围

31.25 pg/mL - 2000 pg/mL

回收率

84% - 110%

平均回收率

1.02

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗IL-1β单克隆抗体的96孔聚苯乙烯酶标板
  • 大鼠IL-1β冻干标准品
  • IL-1β检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-1β抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-1β与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-1β的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat IL-1β ELISA Kit (大鼠白细胞介素1β ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述大鼠 IL1B, IL1-β, IL1F2 靶点信息

IL1B 分子靶点信息概述

  • 分子名:IL1B, interleukin 1 beta
  • 基因家族:Interleukins
  • 别名:IL1F2; IL-1B; IL1-BETA

IL1B 分子靶点综述

白细胞介素1(IL-1)是17kDa的胞外多肽,分为IL-1α和IL-1β两种蛋白。IL-1β是活化的巨噬细胞分泌的蛋白前体,通过caspase1(CASP1/ICE)水解产生的活化形式。该细胞因子是炎症反应的重要介导者,参与许多细胞活性,包括细胞增殖、分化和凋亡。IL-1是一类重要的蛋白家族,它们由生物活化的单核细胞所衍生,参与炎症反应和免疫应答。IL-1β表达升高会产生各种不同的自身炎症综合征,最显著的是单基因条件称为CAPS,是由于触发IL-1β加工的炎性体受体NLRP3发生突变而引起的。本试剂盒用来检测人IL-1β的成熟形式和未裂解的前体形式。

大鼠 Rat Il1b 分子靶点信息

大鼠 Rat Il1b 靶点分子功能(预测)

Enables cytokine activity. Involved in several processes, including positive regulation of intracellular signal transduction; regulation of gene expression; and regulation of neurogenesis. Located in extracellular space. Used to study several diseases, including artery disease (multiple); brain disease (multiple); glomerulonephritis (multiple); interstitial lung disease (multiple); and kidney failure (multiple). Biomarker of several diseases, including artery disease (multiple); auditory system disease (multiple); brain disease (multiple); kidney failure (multiple); and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Orthologous to human IL1B (interleukin 1 beta).

操作步骤


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引用文献

  1. 血清

    Α-Mangostin Alleviated Inflammation in Rats with Adjuvant-Induced Arthritis by Disrupting Adipocytes-Mediated Metabolism-Immune Feedback 

  2. 血清

    Jintiange Capsule Alleviates Rheumatoid Arthritis and Reverses Changes of Serum Metabolic Profile in Collagen-Induced Arthritic Rats 

  3. 全血 心组织 组织匀浆 细胞培养上清

    Red Blood Cell Membrane-Camouflaged PLGA Nanoparticles Loaded With Basic Fibroblast Growth Factor for Attenuating Sepsis-Induced Cardiac Injury 

  4. 组织匀浆 结肠

    Protective role of ergothioneine isolated from Pleurotus ostreatus against dextran sulfate sodium-induced ulcerative colitis in rat model 

  5. 组织匀浆 脊髓背角

    Receptor-Interacting Protein Kinase 3 Inhibition Relieves Mechanical Allodynia and Suppresses NLRP3 Inflammasome and NF-κB in a Rat Model of Spinal Cord Injury 

  6. 眼球玻璃体

    Vision Defense: Efficient Antibacterial AIEgens Induced Early Immune Response for Bacterial Endophthalmitis 

  7. Peroxisome Proliferator-Activated Receptor-Γ Agonist Pioglitazone Ameliorates White Matter Lesion and Cognitive Impairment in Hypertensive Rats 

  8. Overexpression of Egr2 and Egr4 Protects Rat Brains against Ischemic Stroke by Downregulating Jnk Signaling Pathway 

  9. Roflumilast Reduces Cerebral Inflammation in a Rat Model of Experimental Subarachnoid Hemorrhage 

  10. Carnosine Attenuates Brain Oxidative Stress and Apoptosis after Intracerebral Hemorrhage in Rats 

  11. P120-Catenin Mediates Intermittent Cyclic Mechanical Tension-Induced Inflammation in Chondrocytes 

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