Rat IL-1β ELISA Kit 检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥10,800.00
在售SKU:70-EK301B/3-48, 70-EK301B/3-96,70-EK301B/4-48, 70-EK301B/4-96, EK301B-48, EK301B-96, EK301B
- 分子靶点:IL1B
- 种属:大鼠
- 样本类型:血清、血浆、细胞培养上清
- 检测样本体积:血清/血浆:20μL,细胞培养上清:100μL
- 灵敏度:4.48pg/mL
- 检测范围:31.25-2000pg/mL
- 回收率:80%-117%
ELISA试剂盒详细信息
商品名 | Rat IL-1β 酶联免疫检测试剂盒 |
---|---|
种属 | 大鼠 |
靶点 | IL1B |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清 |
检测样本体积 | 血清/血浆:20μL,细胞培养上清:100μL |
灵敏度 | 4.48pg/mL |
线性范围 | 31.25-2000pg/mL |
精密度 | 板内变异系数:3.1%-7.5%;板间变异系数:4.5%-5.5% |
回收率 | 80%-117% |
平均回收率 | 93% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物(TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
IL1B 分子靶点信息概述
- 分子名:IL1B, interleukin 1 beta
- 基因家族:Interleukins
- 别名:IL1F2; IL-1B; IL1-BETA
IL1B 分子靶点综述
白细胞介素1(IL-1)是17kDa的胞外多肽,分为IL-1α和IL-1β两种蛋白。IL-1β是活化的巨噬细胞分泌的蛋白前体,通过caspase1(CASP1/ICE)水解产生的活化形式。该细胞因子是炎症反应的重要介导者,参与许多细胞活性,包括细胞增殖、分化和凋亡。IL-1是一类重要的蛋白家族,它们由生物活化的单核细胞所衍生,参与炎症反应和免疫应答。IL-1β表达升高会产生各种不同的自身炎症综合征,最显著的是单基因条件称为CAPS,是由于触发IL-1β加工的炎性体受体NLRP3发生突变而引起的。本试剂盒用来检测人IL-1β的成熟形式和未裂解的前体形式。
大鼠 Rat Il1b 分子靶点信息
- 分子名:Il1b, interleukin 1 beta
- 别称:
- IL-1 beta
- IL-1F2
- interleukin-1 beta
- 基因序列:NCBI_Gene: 24494
- 蛋白序列:
大鼠 Rat Il1b 靶点分子功能(预测)
Enables cytokine activity. Involved in several processes, including positive regulation of intracellular signal transduction; regulation of gene expression; and regulation of neurogenesis. Located in extracellular space. Used to study several diseases, including artery disease (multiple); brain disease (multiple); glomerulonephritis (multiple); interstitial lung disease (multiple); and kidney failure (multiple). Biomarker of several diseases, including artery disease (multiple); auditory system disease (multiple); brain disease (multiple); kidney failure (multiple); and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Orthologous to human IL1B (interleukin 1 beta).
引用文献统计
该产品被引用的文献总数为:387
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