EasyGo!™ Human IL-10 One-Step ELISA Kit检测试剂盒(酶联免疫吸附法)
¥2,190.00 – ¥3,650.00
在售SKU:70-EK110EG-48, 70-EK110EG-96, EK110EG, 人白细胞介素10, 白细胞介素10, 白细胞介素, 人, 白介素, 10, 高敏
- 分子靶点:IL10
- 种属:human-人
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:50
- 灵敏度:0.19pg/ml
- 检测范围:3.13-200pg/ml
- 回收率:70%-130%
ELISA试剂盒详细信息
商品名 | EasyGo!™ Human IL-10 One-Step ELISA检测试剂盒 |
---|---|
种属 | human-人 |
靶点 | IL10 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 | 50 |
灵敏度 | 0.19pg/ml |
线性范围 | 3.13-200pg/ml |
精密度 | 板内变异系数:< 10%;板间变异系数:< 10% |
回收率 | 70%-130% |
平均回收率 | / |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃ |
运输条件 | blue ice |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上,酶标板孔中预先加入辣根过氧化物酶标记的检测抗体微球。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入显色底物(TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在450nm波长 (参考波长570 - 630nm) 测定吸光度值。 |
分子信息
IL10 分子靶点信息概述
- 分子名:IL10, interleukin 10
- 基因家族:Interleukins
- 别名:CSIF; TGIF; IL10A; IL-10
- 全称:cytokine synthesis inhibitory factor; T-cell growth inhibitory factor
IL10 分子靶点综述
白细胞介素10(IL-10)是由2个长178个氨基酸的亚基构成的同源二聚体。在人类中,它由位于1号染色体的IL-10基因编码,由5个外显子组成。IL-10主要由单核细胞分泌,淋巴细胞、II型辅助性T细胞(Th2)、肥大细胞、CD4+CD25+Foxp3+调节性T细胞和部分活化的T细胞和B细胞分泌较少。它对免疫调控和炎症具有多种效应。它下调巨噬细胞的Th1细胞因子、II类MHC抗原和共刺激分子。它还能增强B细胞存活、增殖和抗体分泌。IL-10能阻断NF-κB活性,并参与JAK-STAT信号通路调控。IL-10的免疫抑制特性提示其可能应用于临床,抑制器官移植后的排斥。IL-10还具有强烈的消炎特性。
人 Human IL10 分子靶点信息
- 分子名:IL10, interleukin 10
- 别称:
- CSIF
- cytokine synthesis inhibitory factor
- GVHDS
- IL-10
- IL10A
- interleukin-10
- MGC126450
- MGC126451
- T-cell growth inhibitory factor
- TGIF
- 基因序列:NCBI_Gene: 3586
- 蛋白序列:UniProtKB: P22301
人 Human IL10靶点分子功能(预测)
Enables protein dimerization activity. Involved in several processes, including negative regulation of cellular catabolic process; regulation of gene expression; and regulation of signal transduction. Acts upstream of or within several processes, including negative regulation of B cell proliferation; positive regulation of B cell apoptotic process; and positive regulation of signaling receptor activity. Located in extracellular space. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Biomarker of several diseases, including autoimmune disease (multiple); diabetes mellitus (multiple); eye disease (multiple); female reproductive organ cancer (multiple); and hematologic cancer (multiple).
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