EK106

Human IL-6 ELISA Kit (人白介素6 ELISA试剂盒)

¥1,600.00¥2,650.00

  • 分子靶点:IL6, IL-6, IFNB2, HGF
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:50 μL
  • 灵敏度:0.37 pg/mL
  • 检测范围:1.56 pg/mL - 100 pg/mL
  • 回收率:99% - 115%

在售SKU:70-EK106/2-48, 70-EK106/2-96, 人白细胞介素6, 白细胞介素6, 白细胞介素, 人, 白介素, 6


描述

商品名

Human IL-6 ELISA Kit (人白介素6 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:1.9% - 4.6%;板间变异系数:2.5%-5.0%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

50 μL

灵敏度

0.37 pg/mL

检测范围

1.56 pg/mL - 100 pg/mL

回收率

99% - 115%

平均回收率

1.06

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗IL-6单克隆抗体的96孔聚苯乙烯酶标板
  • 人IL-6冻干标准品
  • IL-6检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-6抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-6与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-6的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-6 ELISA Kit (人白介素6 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 IL6, IL-6, IFNB2, HGF 靶点信息

IL6 分子靶点信息概述

  • 分子名:IL6, interleukin 6
  • 基因家族:Interferons; Interleukins; Interleukin 6 type cytokine family
  • 别名:IL-6; BSF2; HGF; HSF
  • 曾用名:IFNB2
  • 全称:interferon, beta 2; interleukin 6 (interferon, beta 2)

IL6 分子靶点综述

白细胞介素6(IL-6)是一种同时具备促炎性和抗炎性的细胞因子。在人类中,它由IL-6基因编码。在感染期间和受到外伤后,尤其是烧伤或其它组织损伤导致的炎症,IL-6会由T细胞和巨噬细胞分泌,促进免疫应答。研究表明,小鼠抵抗肺炎链球菌感染时需要IL-6的参与,说明IL-6在抵抗感染中发挥作用。IL-6在很多疾病如糖尿病、动脉粥样硬化、抑郁症、阿尔茨海默氏病、系统性红斑狼疮、多发性骨髓瘤、前列腺癌、白塞氏病和类风湿性关节炎等中可促进炎性和自身免疫疾病进程。晚期/转移性癌症患者的血液中具有较高水平的IL-6。

人 Human IL6 分子靶点信息

  • 分子名:IL6, interleukin 6
  • 别称:
    • B cell stimulatory factor-2
    • B-cell differentiation factor
    • B-cell stimulatory factor 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • HGF
    • HSF
    • hybridoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL-6
    • interferon beta-2
    • interferon, beta 2
    • interleukin BSF-2
    • interleukin-6
  • 基因序列:NCBI_Gene: 3569
  • 蛋白序列:UniProtKB: P05231

人 Human IL6靶点分子功能(预测)

Enables cytokine activity and growth factor activity. Contributes to interleukin-6 receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of macromolecule metabolic process; and regulation of peptide hormone secretion. Acts upstream of or within several processes, including cellular response to lipopolysaccharide; positive regulation of leukocyte adhesion to vascular endothelial cell; and positive regulation of macromolecule metabolic process. Located in extracellular space. Part of interleukin-6 receptor complex. Implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Biomarker of several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and lung disease (multiple).

操作步骤


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引用文献

  1. 细胞培养上清 血清

    Sars-Cov-2 N Protein Promotes Nlrp3 Inflammasome Activation to Induce Hyperinflammation 

  2. 血浆

    Involvement of Impaired Cd8+ Mucosal-Associated Invariant T Cells and Myeloid-Derived Suppressor Cells in Polycystic Ovary Syndrome 

  3. Simvastatin Reduces Myocardial Injury Undergoing Noncoronary Artery Cardiac Surgery: A Randomized Controlled Trial 

  4. Nucleotide-Binding Oligomerization Domain (Nod) Plays an Important Role in Neonatal Infection 

  5. Microrna 27b-3p Modulates Syk in Pediatric Asthma Induced by Dust Mites 

  6. Echinacea Purpurea Extract Polarizes M1 Macrophages in Murine Bone Marrow-Derived Macrophages through the Activation of Jnk 

  7. Distribution of Th17 Cells and Foxp3-Expressing T Cells in Tumor-Infiltrating Lymphocytes in Patients with Uterine Cervical Cancer 

  8. Enhanced Antitumor Effect of Combination of Annexin A1 Knockdown and Bortezomib Treatment in Multiple Myeloma In?vitro and In?vivo 

  9. Berberine Relieves Insulin Resistance Via the Cholinergic Anti-Inflammatory Pathway in Hepg2 Cells 

  10. Harnessing the Cross-Talk between Tumor Cells and Tumor-Associated Macrophages with a Nano-Drug for Modulation of Glioblastoma Immune Microenvironment 

  11. Dendritic Cell-Mediated Delivery of Doxorubicin-Polyglycerol-Nanodiamond Composites Elicits Enhanced Anti-Cancer Immune Response in Glioblastoma 

  12. Anti-Breast Cancer Activity of Spg-56 from Sweet Potato in Mcf-7 Bearing Mice in Situ through Promoting Apoptosis and Inhibiting Metastasis 

  13. Comparison and Relationship of Thyroid Hormones, Il-6, Il-10 and Albumin as Mortality Predictors in Case-Mix Critically Ill Patients 

  14. Different Effects of Lipoteichoic Acid from C. Butyricum and S. Aureus on Inflammatory Responses of Ht-29 Cells 

  15. Microrna-181b Stimulates Inflammation Via the Nuclear Factor-Κb Signaling Pathway in Vitro 

  16. Isobaric Tags for Relative and Absolute Quantitation (Itraq)-Based Proteomic Analysis of Hugan Qingzhi and Its Protective Properties against Free Fatty Acid-Induced L02 Hepatocyte Injury 

  17. Single Administration of Ultra-Low-Dose Lipopolysaccharide in Rat Early Pregnancy Induces Tlr4 Activation in the Placenta Contributing to Preeclampsia 

  18. Γ-Glutamylcysteine Exhibits Anti-Inflammatory Effects by Increasing Cellular Glutathione Level 

  19. Sprouty2 Regulates Proliferation and Survival of Multiple Myeloma By?inhibiting Activation of the Erk1/2 Pathway In?vitro and In?vivo 

  20. Alantolactone Induces G1 Phase Arrest and Apoptosis of Multiple Myeloma Cells and Overcomes Bortezomib Resistance 

  21. Elevated Peripheral Frequencies of Th22 Cells: A Novel Potent Participant in Obesity and Type 2 Diabetes 

  22. Tlr2 Expression Doesn't Change in Ox-Ldl Mediated Inflammation in Human Umbilical Vein Endothelial Cells under High Glucose Culture 

  23. Matrine Increases Nkg2d Ligand Ulbp2 in K562 Cells Via Inhibiting Jak/Stat3 Pathway: A Potential Mechanism Underlying the Immunotherapy of Matrine in Leukemia 

  24. Serum Nox2 as a New Biomarker Candidate for Hbv-Related Disorders 

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