Rat IL-4 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
在售SKU:70-EK304/2-48, 70-EK304/2-96, EK304
- 分子靶点:IL4
- 种属:大鼠
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本20μL
- 灵敏度:0.26pg/mL
- 检测范围:15.63-1000pg/mL
- 回收率:86%-106%
ELISA试剂盒详细信息
商品名 | 大鼠白细胞介素4酶联免疫检测试剂盒 |
---|---|
种属 | 大鼠 |
靶点 | IL4 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本20μL |
灵敏度 | 0.26pg/mL |
线性范围 | 15.63-1000pg/mL |
精密度 | 板内变异系数:2.1%-2.7%;板间变异系数:3.1%-4.3% |
回收率 | 86%-106% |
平均回收率 | 99% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-4抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-4与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-4的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
IL4 分子靶点信息概述
- 分子名:IL4, interleukin 4
- 基因家族:Interleukins
- 别名:BSF1; IL-4; BCGF1; BCGF-1; MGC79402
- 全称:B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; B cell growth factor 1
IL4 分子靶点综述
IL-4是II型辅助T细胞(Th2细胞)分泌的细胞因子。IL-4是一种多效性细胞因子,在调节T、B淋巴细胞和其他类型细胞的增殖、分化、凋亡,促进以Th2细胞为特征的免疫应答过程中发挥重要作用。IL-4多重生物效应依赖于细胞类型和分化状态,研究表明其在介导过敏性疾病、自身免疫性疾病、感染性疾病、肿瘤等疾病的免疫反应中有多重作用,对肿瘤、自身免疫性疾病和感染性疾病等有治疗作用,并且IL-4对疫苗免疫应答具有调节作用。
大鼠 Rat Il4 分子靶点信息
- 分子名:Il4, interleukin 4
- 别称:
- B-cell growth factor 1
- B-cell IGG differentiation factor
- B-cell stimulatory factor 1
- BSF-1
- IL-4
- Il4e12
- interleukin-4
- lymphocyte stimulatory factor 1
- 基因序列:NCBI_Gene: 287287
- 蛋白序列:
大鼠 Rat Il4 靶点分子功能(预测)
Predicted to enable cytokine activity. Involved in several processes, including positive regulation of biosynthetic process; positive regulation of leukocyte migration; and regulation of defense response. Located in extracellular space. Used to study anti-basement membrane glomerulonephritis; autoimmune thyroiditis; and pelvic inflammatory disease. Biomarker of several diseases, including cerebrovascular disease (multiple); interstitial lung disease (multiple); irritant dermatitis; primary biliary cholangitis; and rhinitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); hepatitis B; hepatitis C; and pancreatic cancer (multiple). Orthologous to human IL4 (interleukin 4).
引用文献统计
该产品被引用的文献总数为:35
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