EK3129

Rat CCL5/RANTES ELISA Kit检测试剂盒(酶联免疫吸附法)

¥1,600.00¥2,650.00

在售SKU:70-EK3129-48, 70-EK3129-96, EK3129

  • 分子靶点:CCL5
  • 种属:大鼠
  • 样本类型:血清、血浆、细胞培养上清及其他生物学样本
  • 检测样本体积:血清样本5μL
  • 灵敏度:0.36pg/mL
  • 检测范围:1.56-100pg/mL
  • 回收率:88%-122%

96T

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ELISA试剂盒详细信息

商品名大鼠趋化因子配体5 酶联免疫检测试剂盒
种属大鼠
靶点CCL5
检测方法双抗体夹心法
检测样本类型血清、血浆、细胞培养上清及其他生物学样本
检测样本体积血清样本5μL
灵敏度0.36pg/mL
线性范围1.56-100pg/mL
精密度板内变异系数:2.4%-3.9%;板间变异系数:4.5%-5.2%
回收率88%-122%
平均回收率103%
板式96孔板,可拆
保存条件2-8℃保存。已拆开:标准品-20℃保存,其它4℃。
运输条件2-8℃冰袋运输
检测原理本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物 (TMB) ,避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。

分子信息

概述大鼠 CCL5, RANTES, SISd 靶点信息

CCL5 分子靶点信息概述

  • 分子名:CCL5, C-C motif chemokine ligand 5
  • 基因家族:Chemokine ligands
  • 别名:RANTES; SISd; TCP228; MGC17164
  • 曾用名:D17S136E; SCYA5
  • 全称:T-cell specific protein p288; T-cell specific RANTES protein; SIS-delta; regulated upon activation, normally T-expressed, and presumably secreted; beta-chemokine RANTES; small inducible cytokine subfamily A (Cys-Cys), member 5; small inducible cytokine A5 (RANTES); chemokine (C-C motif) ligand 5

CCL5 分子靶点综述

趋化因子配体5(CCL5),又名RANTES,是一种趋化细胞因子。它可趋化T细胞、嗜酸性粒细胞和嗜碱性粒细胞,在招募白细胞到炎症位点发挥着积极的作用。在T细胞释放的细胞因子如IL-2和IFN-γ的帮助下,CCL5可诱导某些自然杀伤细胞(NK)增殖和活化形成CC趋化因子活化的杀伤(CHAK)细胞。CCL5也是一种由CD8+T细胞释放的HIV抑制因子。最近,研究者正在开发完善乳酸菌体内产生CCL5的方案,以期其成为抑制HIV进入的局部杀菌剂。

大鼠 Rat Ccl5 分子靶点信息

  • 分子名:Ccl5, C-C motif chemokine ligand 5
  • 别称:
    • C-C motif chemokine 5
    • chemokine (C-C motif) ligand 5
    • RANTES
    • regulated upon activation normal T-cell expressed and secreted
    • Scya5
    • SIS-delta
    • small inducible cytokine A5
    • small inducible cytokine A5 (RANTES)
    • small-inducible cytokine A5
    • T-cell-specific protein RANTES
  • 基因序列:NCBI_Gene: 81780
  • 蛋白序列:

大鼠 Rat Ccl5 靶点分子功能(预测)

Enables chemokine activity and heparin binding activity. Involved in several processes, including cellular response to organonitrogen compound; lymphocyte migration; and positive regulation of leukocyte chemotaxis. Located in extracellular space. Used to study Chagas disease; bronchiolitis obliterans; kidney disease; non-alcoholic fatty liver disease; and orchitis. Biomarker of several diseases, including alcoholic hepatitis; artery disease (multiple); cardiac arrest; lung disease (multiple); and uveitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including hepatitis B; hepatitis C; liver disease (multiple); pulmonary tuberculosis; and severe acute respiratory syndrome. Orthologous to human CCL5 (C-C motif chemokine ligand 5).