EK3127

Rat BDNF ELISA Kit检测试剂盒(酶联免疫吸附法)

¥1,600.00¥2,650.00

在售SKU:70-EK3127-48, 70-EK3127-96, EK3127

  • 分子靶点:BDNF
  • 种属:大鼠
  • 样本类型:血清、血浆、细胞培养上清及其他生物学样本
  • 检测样本体积:血清、血浆样本5μL
  • 灵敏度:0.54pg/mL
  • 检测范围:3.91-250pg/mL
  • 回收率:84%-118%

ELISA试剂盒详细信息

商品名Rat BDNF 酶联免疫检测试剂盒
种属大鼠
靶点BDNF
检测方法双抗体夹心法
检测样本类型血清、血浆、细胞培养上清及其他生物学样本
检测样本体积血清、血浆样本5μL
灵敏度0.54pg/mL
线性范围3.91-250pg/mL
精密度板内变异系数:2.7%-4.7%;板间变异系数:3.9%-4.9%
回收率84%-118%
平均回收率105%
板式96孔板,可拆
保存条件2-8℃保存。已拆开:标准品-20℃保存,其它4℃。
运输条件2-8℃冰袋运输
检测原理本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠BDNF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的BDNF与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中BDNF的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。

分子信息

概述大鼠 BDNF, neurotrophin 靶点信息

BDNF 分子靶点信息概述

  • 分子名:BDNF, brain derived neurotrophic factor
  • 基因家族:Neurotrophins
  • 全称:neurotrophin

BDNF 分子靶点综述

脑源性神经营养因子(BDNF)是生长因子神经营养素家族中的一员,与神经生长因子相关。神经营养因子存在于脑及外周。BDNF在内质网中加工合成,由致密核心囊泡分泌。它在大脑中的海马、皮层和基底前脑区域活跃,同样也在视网膜、运动神经元、肾脏、唾液和前列腺中表达。BDNF作用于中枢神经系统和外周神经系统的某些神经元,帮助现存神经元的存活、促进新生神经元和突触的生长和分化。BDNF的改变可能在精神分裂症的发病中起作用,与抑郁、湿疹、癫痫、阿尔茨海默病、肥胖、衰老、先天性中枢性低通气综合征和化疗后认知功能障碍有关。

大鼠 Rat Bdnf 分子靶点信息

大鼠 Rat Bdnf 靶点分子功能(预测)

Enables neurotrophin TRKB receptor binding activity. Involved in several processes, including behavioral response to cocaine; cellular response to norepinephrine stimulus; and regulation of neuronal synaptic plasticity. Acts upstream of or within positive regulation of DNA-binding transcription factor activity; positive regulation of glucocorticoid receptor signaling pathway; and positive regulation of peptidyl-serine phosphorylation. Located in several cellular components, including mitochondrial crista; perikaryon; and synaptic vesicle. Used to study Alzheimer's disease; cystitis; eye disease (multiple); neuropathy (multiple); and status epilepticus. Biomarker of several diseases, including alcohol use disorder; attention deficit hyperactivity disorder; hypertensive encephalopathy; middle cerebral artery infarction; and sciatic neuropathy. Human ortholog(s) of this gene implicated in several diseases, including cognitive disorder (multiple); congenital central hypoventilation syndrome; neurodegenerative disease (multiple); obstructive sleep apnea; and status epilepticus. Orthologous to human BDNF (brain derived neurotrophic factor).

引用文献统计

该产品被引用的文献总数为:7

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