Mouse IL-33 High Sensitivity ELISA Kit检测试剂盒(酶联免疫吸附法)
¥2,000.00 – ¥3,400.00
在售SKU:70-EK233HS-48, 70-EK233HS-96
- 分子靶点:IL33
- 种属:小鼠
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清血浆:20μL;细胞培养上清:100μL
- 灵敏度:0.26pg/mL
- 检测范围:7.81-500pg/mL
- 回收率:80%-102%
ELISA试剂盒详细信息
商品名 | 小鼠白介素33高敏酶联免疫检测试剂盒 |
---|---|
种属 | 小鼠 |
靶点 | IL33 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清血浆:20μL;细胞培养上清:100μL |
灵敏度 | 0.26pg/mL |
线性范围 | 7.81-500pg/mL |
精密度 | 板内变异系数:2.2%-3.2%;板间变异系数:2.6%-3.5% |
回收率 | 80%-102% |
平均回收率 | 87% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-33抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的IL-33与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入信号增强剂孵育,洗涤去除未结合的物质后,再次加入Streptavidin-HRP。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-33的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
IL33 分子靶点信息概述
- 分子名:IL33, interleukin 33
- 基因家族:Interleukins
- 别名:DVS27; DKFZp586H0523; NF-HEV; IL1F11
- 曾用名:C9orf26
- 全称:DVS27-related protein; nuclear factor for high endothelial venules; interleukin-1 family, member 11; chromosome 9 open reading frame 26 (NF-HEV)
IL33 分子靶点综述
白细胞介素33(IL-33)也称为NF-HEV和DVS27,是IL-1家族成员,分子量为30kDa,由多种类型的细胞分泌,包括成纤维细胞、肥大细胞、树突细胞、巨噬细胞、成骨细胞、内皮细胞和上皮细胞。IL-33可诱导辅助性T细胞、肥大细胞、嗜酸性粒细胞和嗜碱性粒细胞分泌II型细胞因子。IL-33通过与受体ST2和IL-1受体辅助蛋白(IL1RAP)结合,活化可促使极化的Th2细胞分泌II型细胞因子(如IL-5和IL-13)的NF-κB和MAP激酶信号通路中的胞外分子来介导其生物学功能。注射IL-33后,其可在体内诱导II型细胞因子,这被认为可诱导粘膜器官发生严重的病理变化。
小鼠 Mouse Il33 分子靶点信息
- 分子名:Il33, interleukin 33
- 别称:
- 9230117N10Rik
- Il-33
- Il1f11
- NF-HEV
- RIKEN cDNA 9230117N10 gene
- 基因序列:NCBI_Gene: 77125
- 蛋白序列:
小鼠 Mouse Il33 靶点分子功能(预测)
Enables cytokine activity and interleukin-33 receptor binding activity. Involved in several processes, including interleukin-33-mediated signaling pathway; microglial cell activation involved in immune response; and regulation of gene expression. Acts upstream of or within several processes, including negative regulation of macrophage proliferation; positive regulation of cellular defense response; and positive regulation of macromolecule metabolic process. Located in nucleus. Is expressed in several structures, including adrenal gland; alimentary system; genitourinary system; nervous system; and sensory organ. Used to study Alzheimer's disease. Human ortholog(s) of this gene implicated in candidiasis; inflammatory bowel disease; and peptic ulcer disease. Orthologous to human IL33 (interleukin 33).
引用文献统计
该产品被引用的文献总数为:16
- Small Proline-Rich Protein 3 Regulates IL-33/ILC2 Axis to Promote Allergic Airway Inflammation
影响因子:8.786刊物:Frontiers in Immunology发表日期:2022/1/20 - Rhein Suppresses Lung Inflammatory Injury Induced by Human Respiratory Syncytial Virus Through Inhibiting NLRP3 Inflammasome Activation via NF-κB Pathway in Mice
影响因子:4.225刊物:Frontiers in Pharmacology发表日期:2020/1/28 - Louki Zupa decoction attenuates the airway inflammation in acute asthma mice induced by ovalbumin through IL-33/ST2-NF-κB/GSK3β/mTOR signalling pathway
影响因子:3.889刊物:PHARMACEUTICAL BIOLOGY发表日期:2022/8/11 - Glial IL-33 signaling through an ST2-to-CXCL12 pathway in the spinal cord contributes to morphine-induced hyperalgesia and tolerance
影响因子:8.218刊物:Science Signaling发表日期:2021/9/7 - Liproxstatin-1 alleviates LPS/IL-13-induced bronchial epithelial cell injury and neutrophilic asthma in mice by inhibiting ferroptosis
影响因子:5.714刊物:INTERNATIONAL IMMUNOPHARMACOLOGY发表日期:2022/4/25 - Tissue-Resident Type 2 Innate Lymphoid Cells Arrest Alveolarization in Bronchopulmonary Dysplasia
影响因子:3.327刊物:Journal of Immunology Research发表日期:2020/10/31 - Therapeutic Effect of Renifolin F on Airway Allergy in an Ovalbumin-Induced Asthma Mouse Model In Vivo
影响因子:4.927刊物:MOLECULES发表日期:2022/6/12 - Fine particulate matter increases airway hyperresponsiveness through kallikrein-bradykinin pathway
影响因子:4.872刊物:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY发表日期:2020/3/23 - Acupuncture inhibited airway inflammation and group 2 innate lymphoid cells in the lung in an ovalbumin-induced murine asthma model
影响因子:2.267刊物:Acupuncture in Medicine发表日期:2021/6/1 - Revealing the regulation of allergic asthma airway epithelial cell inflammation by STEAP4 targeting MIF through machine learning algorithms and single-cell sequencing analysis
影响因子:3.9刊物:Frontiers in Molecular Biosciences发表日期:2024-08-08 - Jiawei Yanghe Decoction attenuate allergic airway inflammation by suppressing group 2 innate lymphoid cells responses
影响因子:4.8刊物:JOURNAL OF ETHNOPHARMACOLOGY发表日期:2024-02-17 - Ultrasmall Nanoparticles Regulate Immune Microenvironment by Activating IL-33/ST2 to Alleviate Renal Ischemia-Reperfusion Injury
影响因子:10刊物:Advanced Healthcare Materials发表日期:2024-02-09 - Radical S-adenosyl methionine domain-containing 2, a potential target of D-tryptophan in asthma treatment, regulates T helper cell type 1/2 balance
影响因子:4.8刊物:INTERNATIONAL IMMUNOPHARMACOLOGY发表日期:2024-02-03 - The egg ribonuclease SjCP1412 accelerates liver fibrosis caused by Schistosoma japonicum infection involving damage associated molecular patterns (DAMPs)
影响因子:2.4刊物:PARASITOLOGY发表日期:2023-12-18 - Effects of total alkaloids from Alstonia scholaris (L.) R. Br. on ovalbumin-induced asthma mice
影响因子:5.4刊物:JOURNAL OF ETHNOPHARMACOLOGY发表日期:2023-07-17 - Silencing SOX11 Alleviates Allergic Rhinitis by Inhibiting Epithelial-Derived Cytokines
影响因子:3刊物:Balkan Medical Journal发表日期:2023-01-23