Mouse IL-2 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
ELISA试剂盒详细信息
商品名 | 小鼠白介素2酶联免疫检测试剂盒 |
---|---|
种属 | 小鼠 |
靶点 | IL2 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清血浆:20μL;细胞培养上清:100μL |
灵敏度 | 0.54pg/mL |
线性范围 | 7.81-500pg/mL |
精密度 | 板内变异系数:3.3%-6.0%;板间变异系数:5.1%-9.0% |
回收率 | 93%-113% |
平均回收率 | 100% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-2与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-2的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
IL2 分子靶点信息概述
- 分子名:IL2, interleukin 2
- 基因家族:Interleukins
- 别名:IL-2; TCGF
- 全称:T cell growth factor
IL2 分子靶点综述
白细胞介素2(IL-2)是15kDa的糖蛋白,在人类中,由位于4号染色体q26–28区域的单基因编码。它是免疫系统中的一类信号分子,调控对具有免疫功能的白血球(白细胞,通常是淋巴细胞)的活性。IL-2是机体对微生物感染的天然应答,区别外来和自身抗原。IL-2通过与IL-2受体来发挥功能,其受体由3条链组成,分别为α链(CD25)、β链(CD122)和γ链(CD132)。监测血清中的IL-2含量为多种病理状态提供更详细的视角,包括癌症、传染病、移植排斥、多发性硬化症、类风湿性关节炎、系统性红斑狼疮和I型糖尿病等。
小鼠 Mouse Il2 分子靶点信息
- 分子名:Il2, interleukin 2
- 别称:
- IL-2
- Il-2
- 基因序列:NCBI_Gene: 16183
- 蛋白序列:
小鼠 Mouse Il2 靶点分子功能(预测)
Enables cytokine activity and interleukin-2 receptor binding activity. Involved in negative regulation of T-helper 17 cell differentiation. Acts upstream of or within several processes, including extrinsic apoptotic signaling pathway in absence of ligand; positive regulation of macromolecule metabolic process; and regulation of lymphocyte activation. Located in extracellular space. Is expressed in liver; omental bursa; thymus; and thymus primordium. Used to study Sjogren's syndrome and inflammatory bowel disease. Human ortholog(s) of this gene implicated in several diseases, including Takayasu's arteritis; auditory system disease (multiple); autoimmune disease (multiple); carcinoma (multiple); and neurodegenerative disease (multiple). Orthologous to human IL2 (interleukin 2).
引用文献统计
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