EK280

Mouse IFN-gamma ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥10,800.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:IFNG, IFN-γ
  • 种属:小鼠 (Mouse)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:50 μL
  • 灵敏度:0.83 pg/mL
  • 检测范围:23.44 pg/mL - 1500 pg/mL
  • 回收率:90% - 108%

在售SKU:70-EK280/3-48, 70-EK280/3-96


描述

商品名

Mouse IFN-γ ELISA Kit (小鼠γ干扰素 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:4.6% - 7.0%;板间变异系数:3.2% - 4.7%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

50 μL

灵敏度

0.83 pg/mL

检测范围

23.44 pg/mL - 1500 pg/mL

回收率

90% - 108%

平均回收率

1.01

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗IFN-γ单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠IFN-γ冻干标准品
  • IFN-γ检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IFN-γ抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IFN-γ与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IFN-γ的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IFN-γ ELISA Kit (小鼠γ干扰素 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 IFNG, IFN-γ 靶点信息

IFNG 分子靶点信息概述

  • 分子名:IFNG, interferon gamma
  • 基因家族:Interferons

IFNG 分子靶点综述

IFN-γ主要由Th1型细胞产生,属于II型细胞因子中干扰素家族中的一员,是一类分泌型糖蛋白,由143个氨基酸组成,相对分子质量是16942。IFN-γ主要由活化的T细胞和NK细胞产生,具有调节机体免疫状态,介导细胞免疫,加强受体介导的吞噬功能,增强巨噬细胞和中性粒细胞的杀伤活性,具有抗病毒感染及抗肿瘤等生物学活性。

小鼠 Mouse Ifng 分子靶点信息

小鼠 Mouse Ifng 靶点分子功能(预测)

Enables cytokine activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of macromolecule metabolic process; and regulation of generation of precursor metabolites and energy. Acts upstream of or within several processes, including CD8-positive, alpha-beta T cell differentiation involved in immune response; defense response to other organism; and positive regulation of macromolecule metabolic process. Located in external side of plasma membrane and extracellular space. Is expressed in several structures, including adipose tissue; alimentary system; genitourinary system; hemolymphoid system gland; and nervous system. Used to study several diseases, including aplastic anemia; hepatitis; malaria; medulloblastoma; and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including allergic rhinitis; ataxia telangiectasia; autoimmune disease (multiple); eczema herpeticum; and leukemia (multiple). Orthologous to human IFNG (interferon gamma).

操作步骤

ELISA操作常见问题

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引用文献

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