Rat IFN-γ High Sensitivity ELISA Kit检测试剂盒(酶联免疫吸附法)
¥2,000.00 – ¥3,400.00
在售SKU:70-EK380HS-48, 70-EK380HS-96
- 分子靶点:IFNG
- 种属:大鼠
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:20μL
- 灵敏度:0.26pg/mL
- 检测范围:3.13-200pg/mL
- 回收率:73%-137%
ELISA试剂盒详细信息
商品名 | 大鼠γ-干扰素高敏酶联免疫检测试剂盒 |
---|---|
种属 | 大鼠 |
靶点 | IFNG |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 20μL |
灵敏度 | 0.26pg/mL |
线性范围 | 3.13-200pg/mL |
精密度 | 板内变异系数:3.7%-4.4%;板间变异系数:2.4%-8.5% |
回收率 | 73%-137% |
平均回收率 | 93% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IFN-γ抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的IFN-γ与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入信号增强剂孵育,洗涤去除未结合的物质后,再次加入Streptavidin-HRP。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IFN-γ的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
IFNG 分子靶点信息概述
- 分子名:IFNG, interferon gamma
- 基因家族:Interferons
IFNG 分子靶点综述
IFN-γ主要由Th1型细胞产生,属于II型细胞因子中干扰素家族中的一员,是一类分泌型糖蛋白,由143个氨基酸组成,相对分子质量是16942。IFN-γ主要由活化的T细胞和NK细胞产生,具有调节机体免疫状态,介导细胞免疫,加强受体介导的吞噬功能,增强巨噬细胞和中性粒细胞的杀伤活性,具有抗病毒感染及抗肿瘤等生物学活性。
大鼠 Rat Ifng 分子靶点信息
- 分子名:Ifng, interferon gamma
- 别称:
- If2f
- IFN-gamma
- IFNG2
- interferon 2f
- 基因序列:NCBI_Gene: 25712
- 蛋白序列:
大鼠 Rat Ifng 靶点分子功能(预测)
Predicted to enable cytokine activity. Involved in several processes, including negative regulation of cell population proliferation; positive regulation of NMDA glutamate receptor activity; and positive regulation of macromolecule metabolic process. Located in several cellular components, including extracellular space; neuron projection; and perikaryon. Used to study several diseases, including Chagas disease; allergic conjunctivitis; allergic rhinitis; renal fibrosis; and ureteral obstruction. Biomarker of several diseases, including abdominal aortic aneurysm; autoimmune thyroiditis; pulpitis; tongue squamous cell carcinoma; and visual epilepsy. Human ortholog(s) of this gene implicated in several diseases, including ataxia telangiectasia; autoimmune disease (multiple); eczema herpeticum; factor VIII deficiency; and leukemia (multiple). Orthologous to human IFNG (interferon gamma).
引用文献统计
该产品被引用的文献总数为:35
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