Mouse IFN-β ELISA Kit检测试剂盒(酶联免疫吸附法)
¥2,000.00 – ¥10,800.00
在售SKU:70-EK2236-48, 70-EK2236-96, EK2236
- 分子靶点:IFNB1
- 种属:小鼠
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本20μL
- 灵敏度:0.83pg/mL
- 检测范围:15.63-1000pg/mL
- 回收率:91%-109%
ELISA试剂盒详细信息
商品名 | 小鼠β干扰素酶联免疫检测试剂盒 |
---|---|
种属 | 小鼠 |
靶点 | IFNB1 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本20μL |
灵敏度 | 0.83pg/mL |
线性范围 | 15.63-1000pg/mL |
精密度 | 板内变异系数:3.1%-4.7%;板间变异系数:3.5%-4.3% |
回收率 | 91%-109% |
平均回收率 | 103% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IFN-β抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IFN-β与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IFN-β的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
IFNB1 分子靶点信息概述
- 分子名:IFNB1, interferon beta 1
- 基因家族:Interferons
- 别名:IFB; IFF
- 曾用名:IFNB
- 全称:interferon, beta 1, fibroblast
IFNB1 分子靶点综述
干扰素(IFNs)是宿主细胞在响应病原体(如病毒、细菌、寄生虫或者肿瘤细胞)时合成和释放的一组信号蛋白。在通常情况下,病毒感染的细胞会释放干扰素,使周围的细胞提高其抗病毒防御能力。基于受体类型,人类干扰素可分为3大类型:I型干扰素,包括IFN-α、IFN-β、IFN-ε、IFN-κ和IFN-ω,II型干扰素(在人类中称为IFN-γ)和III型干扰素。IFN-β通过信号通路STAT1和STAT2上调和下调多种基因,其中,大部分基因参与抗病毒免疫反应。IFN-β在诱导广泛的非特异性抗病毒感染中起重要作用,它还影响细胞增殖并调节免疫应答。
小鼠 Mouse Ifnb1 分子靶点信息
- 分子名:Ifnb1, interferon beta 1, fibroblast
- 别称:
- Ifb
- IFN-beta
- IFNB
- 基因序列:NCBI_Gene: 15977
- 蛋白序列:
小鼠 Mouse Ifnb1 靶点分子功能(预测)
Enables cytokine activity and type I interferon receptor binding activity. Involved in B cell proliferation; cellular response to virus; and type I interferon signaling pathway. Acts upstream of or within several processes, including cellular response to organic cyclic compound; defense response to other organism; and negative regulation of osteoclast differentiation. Located in extracellular space. Is expressed in embryo and liver. Human ortholog(s) of this gene implicated in COVID-19. Orthologous to human IFNB1 (interferon beta 1).
引用文献统计
该产品被引用的文献总数为:54
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