Human TLR2 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥2,000.00 – ¥3,400.00
在售SKU:70-EK1376-48, 70-EK1376-96, EK1376
- 分子靶点:TLR2
- 种属:人
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本10μL
- 灵敏度:3.07pg/mL
- 检测范围:109.38-7,000pg/mL
- 回收率:86%-116%
ELISA试剂盒详细信息
商品名 | 人 TLR2 酶联免疫检测试剂盒 |
---|---|
种属 | 人 |
靶点 | TLR2 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本10μL |
灵敏度 | 3.07pg/mL |
线性范围 | 109.38-7,000pg/mL |
精密度 | 板内变异系数:1.1%-1.4%;板间变异系数:1.2%-1.9% |
回收率 | 86%-116% |
平均回收率 | 94% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物(TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
TLR2 分子靶点信息概述
- 分子名:TLR2, toll like receptor 2
- 基因家族:CD molecules; Toll like receptors
- 别名:TIL4; CD282
- 全称:toll-like receptor 2
TLR2 分子靶点综述
Toll样受体2(TLR2)是一种跨膜受体,可在多种细胞类型(包括小胶质细胞,单核细胞,巨噬细胞和树突状细胞)中表达。TLR组成了模式识别受体家族,在先天免疫反应中起着重要作用。。大量的病原体(例如病毒,细菌和真菌)组成性地表达了一套称为病原体相关分子模式(PAMP)的抗突变分子。这些微生物分子标记物可以由蛋白质,碳水化合物,脂质,核酸和/或其组合组成。单个TLR识别不同的病原体相关PAMP,启动信号级联反应,从而促进免疫反应。
人 Human TLR2 分子靶点信息
- 分子名:TLR2, toll like receptor 2
- 别称:
- CD282
- TIL4
- toll-like receptor 2
- toll/interleukin 1 receptor-like 4
- toll/interleukin-1 receptor-like protein 4
- 基因序列:NCBI_Gene: 7097
- 蛋白序列:UniProtKB: O60603
人 Human TLR2靶点分子功能(预测)
Enables several functions, including lipopolysaccharide binding activity; peptide binding activity; and peptidoglycan binding activity. Involved in several processes, including defense response to other organism; positive regulation of cytokine production; and positive regulation of signal transduction. Acts upstream of or within positive regulation of NF-kappaB transcription factor activity and response to molecule of bacterial origin. Located in several cellular components, including Golgi apparatus; cell surface; and membrane raft. Is intrinsic component of plasma membrane. Part of Toll-like receptor 1-Toll-like receptor 2 protein complex. Is integral component of plasma membrane. Implicated in several diseases, including autoimmune disease (multiple); dermatitis (multiple); gastrointestinal system cancer (multiple); lung disease (multiple); and rhinitis (multiple). Biomarker of several diseases, including autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); middle ear disease (multiple); and nose disease (multiple).