Human sE-Selectin ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
在售SKU:70-EK184-48, 70-EK184-96, EK184
- 分子靶点:SELE
- 种属:人
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:10μL
- 灵敏度:1.94pg/mL
- 检测范围:31.25-2000pg/mL
- 回收率:84%-120%
ELISA试剂盒详细信息
商品名 | 人可溶性E选择素酶联免疫检测试剂盒 |
---|---|
种属 | 人 |
靶点 | SELE |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 10μL |
灵敏度 | 1.94pg/mL |
线性范围 | 31.25-2000pg/mL |
精密度 | 板内变异系数:4.9%-5.4%;板间变异系数:3.7%-7.7% |
回收率 | 84%-120% |
平均回收率 | 102% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人sE-Selectin抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的sE-Selectin与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中sE-Selectin的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
SELE 分子靶点信息概述
- 分子名:SELE, selectin E
- 基因家族:CD molecules; Selectins; Sushi domain containing; C-type lectin domain containing
- 别名:ESEL; CD62E
- 曾用名:ELAM1; ELAM
- 全称:endothelial adhesion molecule 1
SELE 分子靶点综述
E-选择素,又名CD62E、内皮-白细胞粘附分子1(ELAM-1)或者白细胞-内皮细胞粘附分子2(LECAM2),是一个115kDa的糖蛋白,只有被炎性细胞因子(IL-1β或TNF-α)或内皮细胞中的内毒素激活后才表达。细胞表面的E-选择素介导白细胞附着到内皮,从而在局部炎症反应中起重要作用。E-选择素的胞外区包括一个钙依赖性的C2型凝集素结构域、一个表皮生长因子(EGF)结构域和6个重复的补体调节蛋白样序列。可溶性E-选择素(sE-Selectin)存在于健康机体的血液中,可能产生于表面表达分子的蛋白裂解。监测血清中sE-Selectin水平可为一些病理情况如癌症、感染性疾病、糖尿病和脓毒病提供更详细的见解。
人 Human SELE 分子靶点信息
- 分子名:SELE, selectin E
- 别称:
- CD62 antigen-like family member E
- CD62E
- E-selectin
- ELAM
- ELAM-1
- ELAM1
- endothelial adhesion molecule 1
- endothelial leukocyte adhesion molecule 1
- ESEL
- LECAM2
- leukocyte endothelial cell adhesion molecule 2
- leukocyte-endothelial cell adhesion molecule 2
- 基因序列:NCBI_Gene: 6401
- 蛋白序列:UniProtKB: P16581
人 Human SELE靶点分子功能(预测)
Enables several functions, including oligosaccharide binding activity; phospholipase binding activity; and sialic acid binding activity. Involved in several processes, including activation of phospholipase C activity; heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules; and leukocyte tethering or rolling. Located in several cellular components, including caveola; clathrin-coated pit; and perinuclear region of cytoplasm. Is integral component of plasma membrane. Implicated in IgA glomerulonephritis; brain ischemia; cerebrovascular disease; and coronary artery disease. Biomarker of COVID-19; rheumatic myocarditis; and type 1 diabetes mellitus.
引用文献统计
该产品被引用的文献总数为:5
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