Human MMP-2 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
在售SKU:70-EK1M02-48, 70-EK1M02-96, EK1M02
- 分子靶点:MMP2
- 种属:人
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本10μL
- 灵敏度:69.14pg/mL
- 检测范围:2-128ng/mL
- 回收率:86%-118%
ELISA试剂盒详细信息
商品名 | 人基质金属蛋白酶 2酶联免疫检测试剂盒 |
---|---|
种属 | 人 |
靶点 | MMP2 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本10μL |
灵敏度 | 69.14pg/mL |
线性范围 | 2-128ng/mL |
精密度 | 板内变异系数:2.1%-7.8%;板间变异系数:2.4%-3.8% |
回收率 | 86%-118% |
平均回收率 | 108% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人MMP-2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的MMP-2与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中MMP-2的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
MMP2 分子靶点信息概述
- 分子名:MMP2, matrix metallopeptidase 2
- 基因家族:M10 matrix metallopeptidases
- 别名:TBE-1
- 曾用名:CLG4; CLG4A
- 全称:matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
MMP2 分子靶点综述
基质金属蛋白酶2(MMP-2),又名72kDa的IV型胶原酶和明胶酶A,在人类中由MMP2基因编码。激活MMP-2需要蛋白水解加工。MMP-2在子宫内膜破裂、调节血管形成和炎症应答中起作用。MMP-2参与许多事件,如癌症进程、癌细胞浸润、细胞信号转导、新血管形成和淋巴管生成。MMP2基因突变与Torg-Winchester综合征、多发性骨溶解、关节炎综合征有关,可能与瘢痕疙瘩有关。非囊性纤维化支气管扩张中,相比于呼吸道感染铜绿假单胞菌的患者,感染流感嗜血杆菌患者的MMP-2浓度是升高的。
人 Human MMP2 分子靶点信息
- 分子名:MMP2, matrix metallopeptidase 2
- 别称:
- 72 kDa gelatinase
- 72 kDa type IV collagenase
- CLG4
- CLG4A
- collagenase type IV-A
- gelatinase A
- matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
- matrix metalloproteinase 2 (gelatinase a, 72kda gelatinase, 72kda type iv collagenase)
- matrix metalloproteinase-2
- matrix metalloproteinase-II
- MMP-2
- MMP-II
- MONA
- neutrophil gelatinase
- TBE-1
- 基因序列:NCBI_Gene: 4313
- 蛋白序列:UniProtKB: P08253
人 Human MMP2靶点分子功能(预测)
Enables metallopeptidase activity and serine-type endopeptidase activity. Involved in several processes, including cellular response to UV-A; endodermal cell differentiation; and positive regulation of vascular associated smooth muscle cell proliferation. Located in extracellular space. Colocalizes with collagen-containing extracellular matrix. Implicated in artery disease (multiple); eye disease (multiple); pseudoxanthoma elasticum; and temporal arteritis. Biomarker of several diseases, including artery disease (multiple); carcinoma (multiple); cardiomyopathy (multiple); cholesteatoma (multiple); and neurodegenerative disease (multiple).
引用文献统计
该产品被引用的文献总数为:23
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