EK1182

Human ErbB2/HER2/CD340 ELISA Kit检测试剂盒(酶联免疫吸附法)

¥1,600.00¥2,650.00

在售SKU:70-EK1182-48, 70-EK1182-96, EK1182

  • 分子靶点:ERBB2
  • 种属:
  • 样本类型:血清、血浆、细胞培养上清及其他生物学样本
  • 检测样本体积:血清、血浆样本10μL
  • 灵敏度:0.51pg/mL
  • 检测范围:31.25-2000pg/mL
  • 回收率:85%-112%

ELISA试剂盒详细信息

商品名人表皮生长因子受体 2酶联免疫检测试剂盒
种属
靶点ERBB2
检测方法双抗体夹心法
检测样本类型血清、血浆、细胞培养上清及其他生物学样本
检测样本体积血清、血浆样本10μL
灵敏度0.51pg/mL
线性范围31.25-2000pg/mL
精密度板内变异系数:1.9%-3.2%;板间变异系数:2.1%-3.7%
回收率85%-112%
平均回收率106%
板式96孔板,可拆
保存条件2-8℃保存。已拆开:标准品-20℃保存,其它4℃。
运输条件2-8℃冰袋运输
检测原理本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人ErbB2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、检测样本和辣根过氧化物酶标记的检测抗体,经过孵育,样本中存在的ErbB2与固相抗体和检测抗体结合。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中ErbB2的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。

分子信息

概述人 ERBB2, HER2, CD340, NEU 靶点信息

ERBB2 分子靶点信息概述

  • 分子名:ERBB2, erb-b2 receptor tyrosine kinase 2
  • 基因家族:CD molecules; Minor histocompatibility antigens; Erb-b2 receptor tyrosine kinases; MicroRNA protein coding host genes
  • 别名:NEU; HER-2; CD340; HER2
  • 曾用名:NGL
  • 全称:neuro/glioblastoma derived oncogene homolog; human epidermal growth factor receptor 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2

ERBB2 分子靶点综述

ErbB2在人类中由ERBB2基因编码,通常也称之为原癌基因人类表皮生长因子受体2(HER2)或CD340。HER2由胞外配体结合域、跨膜结构域和胞内结构域3部分组成,这些结构域可以和大量信号分子相互作用,体现出配体依赖和非配体依赖性活性。扩增或过表达该致癌基因对某些不良类型的乳腺癌发展有重要作用。近几年来,该蛋白已成为近30%乳腺癌患者生物治疗的一个重要靶点。

人 Human ERBB2 分子靶点信息

  • 分子名:ERBB2, erb-b2 receptor tyrosine kinase 2
  • 别称:
    • c-erb B2/neu protein
    • CD340
    • HER-2
    • HER-2/neu
    • HER2
    • herstatin
    • human epidermal growth factor receptor 2
    • metastatic lymph node gene 19 protein
    • MLN 19
    • NEU
    • neuro/glioblastoma derived oncogene homolog
    • neuroblastoma/glioblastoma derived oncogene homolog
    • NGL
    • p185erbB2
    • proto-oncogene c-ErbB-2
    • proto-oncogene Neu
    • receptor tyrosine-protein kinase erbB-2
    • TKR1
    • tyrosine kinase-type cell surface receptor HER2
    • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
    • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)
    • v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2
    • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
    • VSCN2
  • 基因序列:NCBI_Gene: 2064
  • 蛋白序列:UniProtKB: P04626

人 Human ERBB2靶点分子功能(预测)

Enables several functions, including ErbB-3 class receptor binding activity; enzyme binding activity; and protein C-terminus binding activity. Contributes to growth factor binding activity. Involved in several processes, including positive regulation of macromolecule biosynthetic process; protein phosphorylation; and regulation of MAPK cascade. Acts upstream of or within cell surface receptor signaling pathway. Located in several cellular components, including basolateral plasma membrane; cytosol; and endosome membrane. Part of ERBB3:ERBB2 complex. Implicated in carcinoma (multiple); high grade glioma; reproductive organ cancer (multiple); and urinary bladder cancer. Biomarker of several diseases, including carcinoma (multiple); ductal carcinoma in situ; invasive lobular carcinoma; pre-eclampsia (multiple); and urinary system cancer (multiple).

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