EK1182

Human ErbB2/HER2/CD340 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：ERBB2, HER2, CD340, NEU
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：100 μL
灵敏度：0.51 pg/mL
检测范围：31.25 pg/mL - 2000 pg/mL
回收率：85% - 112%

在售SKU:70-EK1182-48, 70-EK1182-96

描述

文章目录[隐藏]

分子信息
ERBB2 分子靶点信息概述
ERBB2 分子靶点综述
- 人 Human ERBB2 分子靶点信息
- 人 Human ERBB2靶点分子功能（预测）

商品名	Human ErbB2/HER2/CD340 ELISA Kit (人表皮生长因子受体2 (HER2) ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：1.9% - 3.2%；板间变异系数：2.1% - 3.7%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	100 μL
灵敏度	0.51 pg/mL
检测范围	31.25 pg/mL - 2000 pg/mL
回收率	85% - 112%
平均回收率	1.06
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗ErbB2/HER2/CD340单克隆抗体的96孔聚苯乙烯酶标板人ErbB2/HER2/CD340冻干标准品 ErbB2/HER2/CD340检测抗体标准品稀释液辣根过氧化物酶标记的链霉亲和素信号增强剂浓缩液信号增强剂稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人ErbB2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、检测样本和辣根过氧化物酶标记的检测抗体，经过孵育，样本中存在的ErbB2与固相抗体和检测抗体结合。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中ErbB2的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human ErbB2/HER2/CD340 ELISA Kit (人表皮生长因子受体2 (HER2) ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 ERBB2, HER2, CD340, NEU 靶点信息

ERBB2 分子靶点信息概述

分子名：ERBB2, erb-b2 receptor tyrosine kinase 2
基因家族：CD molecules; Minor histocompatibility antigens; Erb-b2 receptor tyrosine kinases; MicroRNA protein coding host genes
别名：NEU; HER-2; CD340; HER2
曾用名：NGL
全称：neuro/glioblastoma derived oncogene homolog; human epidermal growth factor receptor 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2

ERBB2 分子靶点综述

ErbB2在人类中由ERBB2基因编码，通常也称之为原癌基因人类表皮生长因子受体2(HER2)或CD340。HER2由胞外配体结合域、跨膜结构域和胞内结构域3部分组成，这些结构域可以和大量信号分子相互作用，体现出配体依赖和非配体依赖性活性。扩增或过表达该致癌基因对某些不良类型的乳腺癌发展有重要作用。近几年来，该蛋白已成为近30%乳腺癌患者生物治疗的一个重要靶点。

人 Human ERBB2 分子靶点信息

分子名：ERBB2, erb-b2 receptor tyrosine kinase 2
别称：
- c-erb B2/neu protein
- CD340
- HER-2
- HER-2/neu
- HER2
- herstatin
- human epidermal growth factor receptor 2
- metastatic lymph node gene 19 protein
- MLN 19
- NEU
- neuro/glioblastoma derived oncogene homolog
- neuroblastoma/glioblastoma derived oncogene homolog
- NGL
- p185erbB2
- proto-oncogene c-ErbB-2
- proto-oncogene Neu
- receptor tyrosine-protein kinase erbB-2
- TKR1
- tyrosine kinase-type cell surface receptor HER2
- v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
- v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)
- v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2
- v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
- VSCN2
基因序列：NCBI_Gene: 2064
蛋白序列：UniProtKB: P04626

人 Human ERBB2靶点分子功能（预测）

Enables several functions, including ErbB-3 class receptor binding activity; enzyme binding activity; and protein C-terminus binding activity. Contributes to growth factor binding activity. Involved in several processes, including positive regulation of macromolecule biosynthetic process; protein phosphorylation; and regulation of MAPK cascade. Acts upstream of or within cell surface receptor signaling pathway. Located in several cellular components, including basolateral plasma membrane; cytosol; and endosome membrane. Part of ERBB3:ERBB2 complex. Implicated in carcinoma (multiple); high grade glioma; reproductive organ cancer (multiple); and urinary bladder cancer. Biomarker of several diseases, including carcinoma (multiple); ductal carcinoma in situ; invasive lobular carcinoma; pre-eclampsia (multiple); and urinary system cancer (multiple).