Human CCL20/MIP-3α ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
在售SKU:70-EK1211-48, 70-EK1211-96, EK1211
- 分子靶点:CCL20
- 种属:人
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本50μL
- 灵敏度:8.55pg/mL
- 检测范围:15.63-1000pg/mL
- 回收率:87%-119%
ELISA试剂盒详细信息
商品名 | 人趋化因子CC配体20/巨噬细胞炎性蛋白 3α酶联免疫检测试剂盒 |
---|---|
种属 | 人 |
靶点 | CCL20 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本50μL |
灵敏度 | 8.55pg/mL |
线性范围 | 15.63-1000pg/mL |
精密度 | 板内变异系数:3.8%-4.5%;板间变异系数:3.2%-5.3% |
回收率 | 87%-119% |
平均回收率 | 101% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人CCL20抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的CCL20与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中CCL20的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
CCL20 分子靶点信息概述
- 分子名:CCL20, C-C motif chemokine ligand 20
- 基因家族:Chemokine ligands
- 别名:LARC; MIP-3a; exodus-1; ST38; CKb4
- 曾用名:SCYA20
- 全称:small inducible cytokine subfamily A (Cys-Cys), member 20; chemokine (C-C motif) ligand 20
CCL20 分子靶点综述
趋化因子配体20(CCL20),又名巨噬细胞炎性蛋白3α(MIP-3α),是CC趋化因子家族的小分子量细胞因子。CCL20对淋巴细胞具有强烈的趋化作用,而对中性粒细胞的趋化作用较弱。单核细胞、巨噬细胞、小胶质细胞、星形胶质细胞、肥大细胞、角质细胞、肠上皮细胞、成骨细胞、成纤维细胞、内皮细胞和支气管上皮细胞都可表达CCL20。CCL20趋化淋巴细胞和树突状细胞到粘膜淋巴组织周围的上皮细胞,从而参与粘膜淋巴组织的形成和功能的发挥。CCL20结合并激活趋化因子受体CCR6来作用于靶细胞。CCL20在中枢神经系统的自身免疫性疾病发病过程中可能起到重要作用。
人 Human CCL20 分子靶点信息
- 分子名:CCL20, C-C motif chemokine ligand 20
- 别称:
- beta chemokine exodus-1
- beta-chemokine exodus-1
- C-C motif chemokine 20
- CC chemokine LARC
- chemokine (C-C motif) ligand 20
- CKb4
- Exodus
- exodus-1
- LARC
- liver and activation-regulated chemokine
- macrophage inflammatory protein 3 alpha
- MIP-3-alpha
- MIP-3a
- MIP3A
- SCYA20
- small inducible cytokine subfamily A (Cys-Cys), member 20
- small-inducible cytokine A20
- ST38
- 基因序列:NCBI_Gene: 6364
- 蛋白序列:UniProtKB: P78556
人 Human CCL20靶点分子功能(预测)
Enables CCR6 chemokine receptor binding activity. Involved in several processes, including antimicrobial humoral immune response mediated by antimicrobial peptide; calcium-mediated signaling using intracellular calcium source; and cell chemotaxis. Acts upstream of or within positive regulation of T cell migration. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Biomarker of several diseases, including IgA glomerulonephritis; Lyme disease; anogenital venereal wart; dermatitis (multiple); and skin papilloma.
引用文献统计
该产品被引用的文献总数为:6
- Enhance anti-lung tumor efficacy of chimeric antigen receptor-T cells by ectopic expression of C–C motif chemokine receptor 6
影响因子:9.511刊物:Science Bulletin发表日期:2020/12/29 - LR12 Promotes Liver Repair by Improving the Resolution of Inflammation and Liver Regeneration in Mice with Thioacetamide- (TAA-) Induced Acute Liver Failure
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影响因子:7.59刊物:JOURNAL OF INVESTIGATIVE DERMATOLOGY发表日期:2022/7/21 - Identification of CCL20 as a prognostic predictor for severe fever with thrombocytopenia syndrome based on plasma proteomics
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影响因子:5.7刊物:JOURNAL OF INVESTIGATIVE DERMATOLOGY发表日期:2024-01-20 - Association Between Serum Chemokine Ligand 20 Levels and Disease Activity and Th1/Th2/Th17-Related Cytokine Levels in Rheumatoid Arthritis
影响因子:2.3刊物:JOURNAL OF INTERFERON AND CYTOKINE RESEARCH发表日期:2023-10-09