Rat Total MMP-9 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,900.00 – ¥3,200.00
在售SKU:70-EK3M09-48, 70-EK3M09-96, EK3M09
- 分子靶点:MMP9
- 种属:大鼠
- 样本类型:血清、血浆和细胞培养上清
- 检测样本体积:血清、血浆样本10μL
- 灵敏度:37.135pg/mL
- 检测范围:78.13-5000pg/mL
- 回收率:89%-114%
ELISA试剂盒详细信息
商品名 | 大鼠总MMP-9酶联免疫检测试剂盒 |
---|---|
种属 | 大鼠 |
靶点 | MMP9 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆和细胞培养上清 |
检测样本体积 | 血清、血浆样本10μL |
灵敏度 | 37.135pg/mL |
线性范围 | 78.13-5000pg/mL |
精密度 | 板内变异系数:7.7%-9.5%;板间变异系数:5.55%-8.10% |
回收率 | 89%-114% |
平均回收率 | 98% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠totalMMP-9抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和辣根过氧化物酶标记的检测抗体,经过孵育,样本中存在的totalMMP-9与固相抗体和检测抗体结合。洗涤去除未结合的物质后 ,加入显色底物TMB ,避光显色。颜 色反应的深浅与样本中totalMMP-9的浓度成正比。加入终止液终 止反应,在450nm波长 (参考波长570 - 630nm) 测定吸光度值。 |
分子信息
MMP9 分子靶点信息概述
- 分子名:MMP9, matrix metallopeptidase 9
- 基因家族:M10 matrix metallopeptidases
- 曾用名:CLG4B
- 全称:matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
MMP9 分子靶点综述
基质金属蛋白酶9(MMP-9),又名92kDaIV型胶原酶、92kDa明胶酶或者明胶酶B,是一类属于锌-金属蛋白酶家族的酶。MMP-9参与正常生理过程(如胚胎发育、繁殖、血管形成、骨骼发育、伤口愈细胞迁移、学习和记忆)和病理过程(如关节炎、脑出血和肿瘤转移)的细胞外基质降解。MMP-9和弹性蛋白酶似乎是中性粒细胞迁移通过基底膜的调节因子。MMP-9在血管和新生血管形成中起重要作用。它在人类呼吸道上皮愈合过程中被显著上调,并被发现与许多病理过程相关,如癌症、免疫和心血管疾病。在类风湿性关节炎、局灶性脑缺血案例中可发现MMP-9的水平是升高的。它的水平随着特发性房颤的发展而增加。
大鼠 Rat Mmp9 分子靶点信息
- 分子名:Mmp9, matrix metallopeptidase 9
- 别称:
- 92 kDa gelatinase
- 92 kDa type IV collagenase
- 92-kDa type IV collagenase
- gelatinase B
- GELB
- matrix metalloproteinase 9 (gelatinase B 92-kDa type IV collagenase)
- matrix metalloproteinase 9 (gelatinase B, 92-kDa type IV collagenase)
- matrix metalloproteinase-9
- MMP-9
- 基因序列:NCBI_Gene: 81687
- 蛋白序列:
大鼠 Rat Mmp9 靶点分子功能(预测)
Enables fibronectin binding activity and metallopeptidase activity. Involved in several processes, including cellular response to cell-matrix adhesion; cellular response to cytokine stimulus; and cellular response to iron(III) ion. Located in extracellular space. Part of protein-containing complex. Used to study artery disease (multiple); brain ischemia (multiple); congestive heart failure; glomerulosclerosis; and lung disease (multiple). Biomarker of several diseases, including artery disease (multiple); brain disease (multiple); chronic obstructive pulmonary disease (multiple); glucose metabolism disease (multiple); and kidney disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including Down syndrome; anodontia; artery disease (multiple); autoimmune disease (multiple); and chronic obstructive pulmonary disease (multiple). Orthologous to human MMP9 (matrix metallopeptidase 9).
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