EK387

Rat JE/MCP-1/CCL2 ELISA Kit检测试剂盒(酶联免疫吸附法)

¥2,000.00¥3,400.00

  • 分子靶点:CCL2, MCP-1, MCAF, SMC-CF
  • 种属:大鼠 (Rat)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:100 μL(稀释液)
  • 灵敏度:0.50 pg/mL
  • 检测范围:7.81 pg/mL - 500 pg/mL
  • 回收率:84% - 110%

在售SKU:70-EK387-48, 70-EK387-96


描述

商品名

Rat JE/MCP-1/CCL2 ELISA Kit (大鼠 JE/MCP-1/CCL2 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:4.3% - 5.0%;板间变异系数:4.3%-9.4%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

100 μL(稀释液)

灵敏度

0.50 pg/mL

检测范围

7.81 pg/mL - 500 pg/mL

回收率

84% - 110%

平均回收率

1.03

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗大鼠CCL2单克隆抗体的96孔聚苯乙烯酶标板
  • 大鼠CCL2冻干标准品
  • 生物素标记的大鼠CCL2检测抗体
  • 辣根过氧化物酶标记的链霉亲和素
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠CCL2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、检测样本和辣根过氧化物酶标记的检测抗体,经过孵育,样本中存在的CCL2与固相抗体和检测抗体结合。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中CCL2的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat JE/MCP-1/CCL2 ELISA Kit (大鼠 JE/MCP-1/CCL2 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述大鼠 CCL2, MCP-1, MCAF, SMC-CF 靶点信息

CCL2 分子靶点信息概述

  • 分子名:CCL2, C-C motif chemokine ligand 2
  • 基因家族:Chemokine ligands
  • 别名:MCP1; MCP-1; MCAF; SMC-CF; GDCF-2; HC11; MGC9434
  • 曾用名:SCYA2
  • 全称:monocyte chemotactic protein 1, homologous to mouse Sig-je; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte secretory protein JE; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); chemokine (C-C motif) ligand 2

CCL2 分子靶点综述

趋化因子配体2(CCL2),又名单核细胞趋化蛋白1(MCP-1),是CC趋化因子家族的小分子量细胞因子。CCL2通过蛋白聚糖的粘多糖侧链锚定到内皮细胞的质膜上,主要由单核细胞、巨噬细胞和树突状细胞分泌。CCL2可招募单核细胞、记忆性T细胞和树突状细胞到组织损伤或感染引起的炎症位点。CCL2参与以单核细胞浸润为特征的一些疾病发病机制,如银屑病、类风湿性关节炎和动脉粥样硬化。CCL2参与发生在中枢神经系统的多种疾病的神经炎症过程,表现为神经元变性。

大鼠 Rat Ccl2 分子靶点信息

  • 分子名:Ccl2, C-C motif chemokine ligand 2
  • 别称:
    • C-C motif chemokine 2
    • chemokine (C-C motif) ligand 2
    • immediate-early serum-responsive JE protein
    • immediate-early serum-responsive protein JE
    • MCP-1
    • monocyte chemoattractant protein 1
    • monocyte chemoattractant protein-1
    • monocyte chemotactic protein 1
    • Scya2
    • Sigje
    • small inducible cytokine A2
    • Small inducible gene JE
    • small-inducible cytokine A2
  • 基因序列:NCBI_Gene: 24770
  • 蛋白序列:UniProtKB: P14844

大鼠 Rat Ccl2 靶点分子功能(预测)

Enables chemokine activity and heparin binding activity. Involved in several processes, including cellular response to cytokine stimulus; cellular response to lipid; and leukocyte migration. Located in several cellular components, including perikaryon; perinuclear region of cytoplasm; and rough endoplasmic reticulum. Colocalizes with C-fiber. Used to study several diseases, including artery disease (multiple); diabetic angiopathy; interstitial lung disease (multiple); kidney disease (multiple); and pancreatitis (multiple). Biomarker of several diseases, including artery disease (multiple); cerebrovascular disease (multiple); lung disease (multiple); non-alcoholic fatty liver disease (multiple); and pancreatitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including Henoch-Schoenlein purpura; aggressive periodontitis; autoimmune disease (multiple); gastrointestinal system cancer (multiple); and tuberculosis (multiple). Orthologous to human CCL2 (C-C motif chemokine ligand 2).

操作步骤


下载本产品说明书

ELISA操作常见问题

查看更多ELISA操作相关问题

引用文献


  1. 血清

    Α-Mangostin Alleviated Inflammation in Rats with Adjuvant-Induced Arthritis by Disrupting Adipocytes-Mediated Metabolism-Immune Feedback 

评价

目前还没有评价

成为第一个“Rat JE/MCP-1/CCL2 ELISA Kit检测试剂盒(酶联免疫吸附法)” 的评价者

您的电子邮箱地址不会被公开。 必填项已用*标注