EK306

Rat IL-6 ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥10,800.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:IL6, IL-6, IFNB2, HGF
  • 种属:大鼠 (Rat)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:20 μL
  • 灵敏度:2.57 pg/mL
  • 检测范围:31.25 pg/mL - 2000 pg/mL
  • 回收率:92% - 104%

在售SKU:70-EK306/3-48, 70-EK306/3-96


描述

商品名

Rat IL-6 ELISA Kit (大鼠白细胞介素6 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:4.2% - 7.7%;板间变异系数:6.3% - 9.1%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

20 μL

灵敏度

2.57 pg/mL

检测范围

31.25 pg/mL - 2000 pg/mL

回收率

92% - 104%

平均回收率

0.97

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗IL-6单克隆抗体的96孔聚苯乙烯酶标板
  • 大鼠IL-6冻干标准品
  • IL-6检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-6抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-6与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-6的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat IL-6 ELISA Kit (大鼠白细胞介素6 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述大鼠 IL6, IL-6, IFNB2, HGF 靶点信息

IL6 分子靶点信息概述

  • 分子名:IL6, interleukin 6
  • 基因家族:Interferons; Interleukins; Interleukin 6 type cytokine family
  • 别名:IL-6; BSF2; HGF; HSF
  • 曾用名:IFNB2
  • 全称:interferon, beta 2; interleukin 6 (interferon, beta 2)

IL6 分子靶点综述

白细胞介素6(IL-6)是一种同时具备促炎性和抗炎性的细胞因子。在人类中,它由IL-6基因编码。在感染期间和受到外伤后,尤其是烧伤或其它组织损伤导致的炎症,IL-6会由T细胞和巨噬细胞分泌,促进免疫应答。研究表明,小鼠抵抗肺炎链球菌感染时需要IL-6的参与,说明IL-6在抵抗感染中发挥作用。IL-6在很多疾病如糖尿病、动脉粥样硬化、抑郁症、阿尔茨海默氏病、系统性红斑狼疮、多发性骨髓瘤、前列腺癌、白塞氏病和类风湿性关节炎等中可促进炎性和自身免疫疾病进程。晚期/转移性癌症患者的血液中具有较高水平的IL-6。

大鼠 Rat Il6 分子靶点信息

  • 分子名:Il6, interleukin 6
  • 别称:
    • Ifnb2
    • IL-6
    • ILg6
    • Interleukin 6 (interferon beta 2)
    • Interleukin 6 (interferon, beta 2)
    • interleukin-6
  • 基因序列:NCBI_Gene: 24498
  • 蛋白序列:UniProtKB: P20607

大鼠 Rat Il6 靶点分子功能(预测)

Enables cytokine activity and interleukin-6 receptor binding activity. Involved in several processes, including cellular response to lipid; positive regulation of cell communication; and response to peptide hormone. Located in cytoplasm and extracellular space. Used to study several diseases, including acute necrotizing pancreatitis; disseminated intravascular coagulation; fatty liver disease; hypertension (multiple); and impotence. Biomarker of several diseases, including acute necrotizing pancreatitis; artery disease (multiple); auditory system disease (multiple); iron deficiency anemia; and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Orthologous to human IL6 (interleukin 6).

操作步骤

ELISA操作常见问题

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Imperatorin alleviated endometriosis by inhibiting the activation of PI3K/Akt/NF-κB pathway in rats 

  2. Pyrogallol enhances therapeutic effect of human umbilical cord mesenchymal stem cells against LPS-mediated inflammation and lung injury via activation of Nrf2/HO-1 signaling 

  3. Propofol pretreatment alleviates mast cell degranulation by inhibiting SOC to protect the myocardium from ischemia–reperfusion injury 

  4. Suppression of NOD-like receptor protein 3 inflammasome activation and macrophage M1 polarization by hederagenin contributes to attenuation of sepsis-induced acute lung injury in rats 

  5. Metabolomics and pharmacodynamic analysis reveal the therapeutic role of Prunella vulgaris oil on intrauterine adhesion rats 

  6. Salt sensitive purely zwitterionic physical hydrogel for prevention of postoperative tissue adhesion 

  7. Analysis of the Potential Relationship between Aging and Pulmonary Fibrosis Based on Transcriptome 

  8. Timosaponin B-II alleviates osteoarthritis-related inflammation and extracellular matrix degradation through inhibition of mitogen-activated protein kinases and nuclear factor-κB pathways in vitro 

  9. Reconfiguration of Gut Microbiota and Reprogramming of Liver Metabolism with Phycobiliproteins Bioactive Peptides to Rehabilitate Obese Rats 

  10. α-Mangostin Alleviated Inflammation in Rats With Adjuvant-Induced Arthritis by Disrupting Adipocytes-Mediated Metabolism-Immune Feedback 

  11. The potential mechanism of huazhuojiedu decoction in the treatment of ulcerative colitis based on network pharmacology and experimental validation 

  12. Qing-Luo-Yin Alleviated Monocytes/Macrophages-Mediated Inflammation in Rats with Adjuvant-Induced Arthritis by Disrupting Their Interaction with (Pre)-Adipocytes Through PPAR-γ Signaling 

  13. Astragaloside IV Protects from PM2.5-Induced Lung Injury by Regulating Autophagy via Inhibition of PI3K/Akt/mTOR Signaling in vivo and in vitro 

  14. Calcium–Zinc Phosphate Chemical Conversion Coating Facilitates the Osteointegration of Biodegradable Zinc Alloy Implants by Orchestrating Macrophage Phenotype 

  15. Xianglian pill modulates gut microbial production of succinate and induces regulatory T cells to alleviate ulcerative colitis in rats 

  16. Improvement of bone formation by bionic hydroxyapatite nanorod via the regulation of macrophage polarization 

  17. Andrographolide exerted anti-inflammatory effects thereby reducing sex hormone synthesis in LPS-induced female rats, but had no effect on hormone production in healthy ones 

  18. The short-chain fatty acid propionate improved ventricular electrical remodeling in a rat model with myocardial infarction 

  19. Cinnamaldehyde Promotes the Intestinal Barrier Functions and Reshapes Gut Microbiome in Early Weaned Rats 

  20. Downregulation of miR-892b inhibits the progression of osteoarthritis via targeting cyclin D1 and cyclin D2 

  21. Chemical profiles of the active fraction from Prinsepia utilis Royle leaves and its anti-benign prostatic hyperplasia evaluation in animal models 

  22. Probiotics ameliorates pulmonary inflammation via modulating gut microbiota and rectifying Th17/Treg imbalance in a rat model of PM2.5 induced lung injury 

  23. Overexpression of TOLLIP Protects against Acute Kidney Injury after Paraquat Intoxication through Inhibiting NLRP3 Inflammasome Activation Modulated by Toll-Like Receptor 2/4 Signaling 

  24. A tannic acid doped hydrogel with small extracellular vesicles derived from mesenchymal stem cells promotes spinal cord repair by regulating reactive oxygen species microenvironment 

  25. The therapeutic effects of Prunella vulgaris against fluoride-induced oxidative damage by using the metabolomics method 

  26. MicroRNA-342-3p loaded by human umbilical cord mesenchymal stem cells-derived exosomes attenuates deep vein thrombosis by downregulating EDNRA 

  27. Neuroprotective effects of macamide from maca (Lepidium meyenii Walp.) on corticosterone-induced hippocampal impairments through its anti-inflammatory, neurotrophic, and synaptic protection properties 

  28. β‑estradiol alleviates hypertension‑ and concanavalin A‑mediated inflammatory responses via modulation of connexins in peripheral blood lymphocytes 

  29. Genomic G-quadruplex folding triggers a cytokine-mediated inflammatory feedback loop to aggravate inflammatory diseases 

  30. Plantaginis Semen polysaccharides ameliorate renal damage through regulating NLRP3 inflammasome in gouty nephropathy rats 

  31. Astragaloside IV alleviates PM2.5-induced lung injury in rats by modulating TLR4/MyD88/NF-κB signalling pathway 

  32. The dynamic changes in autophagy activity and its role in lung injury after deep hypothermic circulatory arrest 

  33. Gut derived-endotoxin contributes to inflammation in severe ischemic acute kidney injury 

  34. A metabonomics and lipidomics based network pharmacology study of qi-tonifying effects of honey-processed Astragalus on spleen qi deficiency rats 

  35. Safety evaluation and protective effects of ethanolic extract from maca (Lepidium meyenii Walp.) against corticosterone and H2O2 induced neurotoxicity 

  36. Tibetan medicine Ershiwuwei Lvxue Pill attenuates collagen-induced arthritis via inhibition of JAK2/STAT3 signaling pathway 

  37. In Situ Regulation of Macrophage Polarization to Enhance Osseointegration Under Diabetic Conditions Using Injectable Silk/Sitagliptin Gel Scaffolds 

  38. Telmisartan ameliorates LPS-induced pneumonia in rats through regulation of the PPARγ/NF-κB pathway 

  39. Propionate alleviated post-infarction cardiac dysfunction by macrophage polarization in a rat model 

  40. Tanshinone I Mitigates Steroid-Induced Osteonecrosis of the Femoral Head and Activates the Nrf2 Signaling Pathway in Rats 

  41. CD39/CD73/A2a Adenosine Metabolic Pathway: Targets for Moxibustion in Treating DSS-Induced Ulcerative Colitis 

  42. Protective effects of icariin on dorsal random skin flap survival: An experimental study 

  43. Bioactive fractions from Securidaca inappendiculata alleviated collagen-induced arthritis in rats by regulating metabolism-related signaling 

  44. Supercritical Fluid-Assisted Porous Microspheres for Efficient Delivery of Insulin and Inhalation Therapy of Diabetes 

  45. Curcumin inhibits cigarette smoke-induced inflammation via modulating the PPARγ-NF-κB signaling pathway 

  46. Downregulation of lncRNA SNHG14 attenuates osteoarthritis by inhibiting FSTL-1 mediated NLRP3 and TLR4/NF-κB pathway through miR-124-3p 

  47. Overexpression of miR-146a-5p Ameliorates Inflammation and Autophagy in TLCs-Induced AR42J Cell Model of Acute Pancreatitis by Inhibiting IRAK1/TRAF6/NF-κB Pathway 

  48. Therapeutic Effect and Mechanism Study of Rhodiola wallichiana var. cholaensis Injection to Acute Blood Stasis Using Metabolomics Based on UPLC-Q/TOF-MS 

  49. LXA4 protects against hypoxic-ischemic damage in neonatal rats by reducing the inflammatory response via the IκB/NF-κB pathway 

  50. Hyaluronic acid coated bilirubin nanoparticles attenuate ischemia reperfusion-induced acute kidney injury 

  51. Effects and mechanism of stem cells from human exfoliated deciduous teeth combined with hyperbaric oxygen therapy in type 2 diabetic rats 

  52. The effect of Metapanax delavayi leaf extract on testosterone‐induced benign prostatic hyperplasia in rats 

  53. Gastroprotective Effect of Ethanol Extracts from Bark of Magnolia officinalis on Ethanol-Induced Gastric Mucosal Damage in Rats 

  54. Pi-Dan-Jian-Qing Decoction Ameliorates Type 2 Diabetes Mellitus Through Regulating the Gut Microbiota and Serum Metabolism 

  55. The Preparation of a Novel Poly(Lactic Acid)-Based Sustained H2S Releasing Microsphere for Rheumatoid Arthritis Alleviation 

  56. A novel dendritic mesoporous silica based sustained hydrogen sulfide donor for the alleviation of adjuvant-induced inflammation in rats 

  57. Chronic microglial inflammation promotes neuronal lactate supply but impairs its utilization in primary rat astrocyte-neuron co-cultures 

  58. Assessment of pulmonary toxicity of potential antioxidant drug PEGylated nanoceria after intratracheal instillation in rats 

  59. Puerarin improves the bone micro-environment to inhibit OVX-induced osteoporosis via modulating SCFAs released by the gut microbiota and repairing intestinal mucosal integrity 

  60. Sanguinarine Attenuates Neuropathic Pain in a Rat Model of Chronic Constriction Injury 

  61. The protective effect of hypericin on postpartum depression rat model by inhibiting the NLRP3 inflammasome activation and regulating glucocorticoid metabolism 

  62. D-dopachrome tautomerase drives astroglial inflammation via NF-κB signaling following spinal cord injury 

  63. Sanguinarine Attenuates Neuropathic Pain by Inhibiting P38 MAPK Activated Neuroinflammation in Rat Model 

  64. Shenling Baizhu Powder Alleviates TNBS-Induced Colitis in Rats by Improving Intestinal Epithelial Permeability and Inhibiting Inflammation Through the TLR5/MyD88/NF-κB Pathway. 

  65. Transcription factor nuclear factor erythroid 2 p45-related factor 2 (NRF2) ameliorates sepsis-associated acute kidney injury by maintaining mitochondrial homeostasis and improving the mitochondrial function 

  66. Solid phase synthesis of oxidized sodium alginate-tobramycin conjugate and its application for infected wound healing 

  67. Tanshinone IIA ameliorates non-alcoholic fatty liver disease through targeting peroxisome proliferator-activated receptor gamma and toll-like receptor 4 

  68. Indoleamine 2,3-dioxygenase is upregulated in the brain of rats with acute pancreatitis 

  69. Galunisertib-Loaded Gelatin Methacryloyl Hydrogel Microneedle Patch for Cardiac Repair after Myocardial Infarction 

  70. SMYD1 alleviates septic myocardial injury by inhibiting endoplasmic reticulum stress 

  71. Cholinergic Elicitation Prevents Ventricular Remodeling via Alleviations of Myocardial Mitochondrial Injury Linked to Inflammation in Ischemia-Induced Chronic Heart Failure Rats 

  72. The Therapeutic Efficacy of Adipose Tissue-Derived Mesenchymal Stem Cell Conditioned Medium on Experimental Colitis Was Improved by the Serum From Colitis Rats 

  73. Discovery and In Vivo Anti-inflammatory Activity Evaluation of a Novel Non-peptidyl Non-covalent Cathepsin C Inhibitor 

  74. Qing-Luo-Yin Alleviated Experimental Arthritis in Rats by Disrupting Immune Feedback Between Inflammatory T Cells and Monocytes: Key Evidences from Its Effects on Immune Cell Phenotypes 

  75. Fucoidan ameliorates glucose metabolism by the improvement of intestinal barrier and inflammatory damage in type 2 diabetic rats 

  76. San-Huang-Yi-Shen Capsule Ameliorates Diabetic Kidney Disease through Inducing PINK1/Parkin-Mediated Mitophagy and Inhibiting the Activation of NLRP3 Signaling Pathway 

  77. Structural modulation of gut microbiota during alleviation of non-alcoholic fatty liver disease with Gynostemma pentaphyllum in rats 

  78. IL-17 crosses the blood–brain barrier to trigger neuroinflammation: a novel mechanism in nitroglycerin-induced chronic migraine 

  79. AMPK/SIRT1 Deficiency Drives Adjuvant-Induced Arthritis in Rats by Promoting Glycolysis-Mediated Monocytes Inflammatory Polarization 

  80. Influences of Xylitol Consumption at Different Dosages on Intestinal Tissues and Gut Microbiota in Rats 

  81. Umbilical Cord Mesenchymal Stem Cells for Inflammatory Regulation After Excision and Grafting of Severe Burn Wounds in Rats 

  82. Polydopamine-Decorated Microcomposites Promote Functional Recovery of an Injured Spinal Cord by Inhibiting Neuroinflammation 

  83. Smoking increases oral mucosa susceptibility to Candida albicans infection via the Nrf2 pathway: In vitro and animal studies 

  84. The Protective Effect of Sulodexide on Acute Lung Injury Induced by a Murine Model of Obstructive Jaundice 

  85. The effects of Saccharomyces boulardii on rat colonic hypermotility induced by repeated water avoidance stress and the potential mechanism 

  86. MKK4 Knockdown Plays a Protective Role in Hemorrhagic Shock-Induced Liver Injury through the JNK Pathway 

  87. Curcumin improves LPS-induced preeclampsia-like phenotype in rat by inhibiting the TLR4 signaling pathway 

  88. Metformin Regulates Alveolar Macrophage Polarization to Protect Against Acute Lung Injury in Rats Caused by Paraquat Poisoning 

  89. Paraquat promotes acute lung injury in rats by regulating alveolar macrophage polarization through glycolysis 

  90. Nanoparticle-assembled bioadhesive coacervate coating with prolonged gastrointestinal retention for inflammatory bowel disease therapy 

  91. The self-nanoemulsifying drug delivery system of Petiveria alliacea extract reduced the homeostatic model assessment-insulin resistance value, interleukin-6, and tumor necrosis factor-α level in diabetic rat models 

  92. The Anti-Depressive Effects of Hesperidin and the Relative Mechanisms Based on the NLRP3 Inflammatory Signaling Pathway 

  93. Ischemic postconditioning ameliorates acute kidney injury induced by limb ischemia/reperfusion via transforming TLR4 and NF-κB signaling in rats 

  94. Celastrol ameliorates osteoarthritis via regulating TLR2/NF-κB signaling pathway. 

  95. Forsythiae Fructuse water extract attenuates liver fibrosis via TLR4/MyD88/NF-κB and TGF-β/smads signaling pathways 

  96. Gut microbiota and metabonomics used to explore the mechanism of Qing’e Pills in alleviating osteoporosis 

  97. PTPRO knockdown protects against inflammation in hemorrhage shock-induced lung injury involving the NF-κB signaling pathway 

  98. Comparisons of Chemical Profiles and Gastroprotective Effects of Citri Sarcodactylis Fructus Pre- and Poststeam Processing 

  99. MicroRNA-181b Inhibits Inflammatory Response and Reduces Myocardial Injury in Sepsis by Downregulating HMGB1 

  100. miR-29a-3p in Exosomes from Heme Oxygenase-1 Modified Bone Marrow Mesenchymal Stem Cells Alleviates Steatotic Liver Ischemia-Reperfusion Injury in Rats by Suppressing Ferroptosis via Iron Responsive Element Binding Protein 2 

  101. Bio-inspired, bio-degradable adenosine 5′-diphosphate-modified hyaluronic acid coordinated hydrophobic undecanal-modified chitosan for hemostasis and wound healing 

  102. Structural characteristics and anti-inflammatory activity of UV/H2O2-treated algal sulfated polysaccharide from Gracilaria lemaneiformis 

  103. Guizhi Fuling Wan, Chinese Herbal Medicine, Ameliorates Insulin Sensitivity in PCOS Model Rats With Insulin Resistance via Remodeling Intestinal Homeostasis 

  104. Knockdown of hsa_circ_0037658 inhibits the progression of osteoarthritis via inducing autophagy 

  105. Chinese herbal formula Fuzheng Huayu alleviates CCl4-induced liver fibrosis in rats: a transcriptomic and proteomic analysis 

  106. Ilexgenin A enhances the effects of simvastatin on non-alcoholic fatty liver disease without changes in simvastatin pharmacokinetics 

  107. Dexmedetomidine-induced cardioprotection is mediated by inhibition of high mobility group box-1 and the cholinergic anti-inflammatory pathway in myocardial ischemia-reperfusion injury 

  108. HDL-S1P protects endothelial function and reduces lung injury during sepsis in vivo and in vitro 

  109. Dexmedetomidine preconditioning may attenuate myocardial ischemia/reperfusion injury by down-regulating the HMGB1-TLR4-MyD88-NF-кB signaling pathway 

  110. Cardioprotective Effects of HuoxueAnshen Recipe against Myocardial Injuries Induced by Sleep Deprivation in Rats 

  111. Human amnion-derived mesenchymal stem cells alleviate lung injury induced by white smoke inhalation in rats 

  112. Paeoniflorin improves cardiac function and decreases adverse postinfarction left ventricular remodeling in a rat model of acute myocardial infarction 

  113. Protection Against Post-resuscitation Acute Kidney Injury by N-Acetylcysteine via Activation of the Nrf2/HO-1 Pathway 

  114. Selenium/Zinc-Enriched probiotics improve serum enzyme activity, antioxidant ability, inflammatory factors and related gene expression of Wistar rats inflated under heat stress 

  115. The Effect of a Compound Protein on Wound Healing and Nutritional Status 

  116. Сardiac injury in rats with experimental posttraumatic stress disorder and mechanisms of its limitation in experimental posttraumatic stress disorder-resistant rats 

  117. Effects of prebiotic galacto-oligosaccharide on postoperative cognitive dysfunction and neuroinflammation through targeting of the gut-brain axis 

  118. Serum untargeted metabolomics analysis of the mechanisms of evodiamine on type 2 diabetes mellitus model rats 

  119. Safety considerations of chloroquine in the treatment of patients with diabetes and COVID-19 

  120. Thrombin induces morphological and inflammatory astrocytic responses via activation of PAR1 receptor 

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