Rat C-Reactive Protein/CRP ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
在售SKU:70-EK394-48, 70-EK394-96, EK394
- 分子靶点:CRP
- 种属:大鼠
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本10μL
- 灵敏度:3.13pg/mL
- 检测范围:46.88-3000pg/mL
- 回收率:80%-121%
ELISA试剂盒详细信息
商品名 | 大鼠C-反应蛋白酶联免疫检测试剂盒 |
---|---|
种属 | 大鼠 |
靶点 | CRP |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本10μL |
灵敏度 | 3.13pg/mL |
线性范围 | 46.88-3000pg/mL |
精密度 | 板内变异系数:4.6%-5.0%;板间变异系数:5.7%-6.6% |
回收率 | 80%-121% |
平均回收率 | 108% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠CRP抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的CRP与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中CRP的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
CRP 分子靶点信息概述
- 分子名:CRP, C-reactive protein
- 基因家族:Short pentraxins
- 别名:PTX1
- 全称:pentraxin 1; C-reactive protein, pentraxin-related
CRP 分子靶点综述
C-反应蛋白(CRP)是在血浆中发现的一种环状、五聚体蛋白。肝脏对巨噬细胞和脂肪细胞释放的因子产生应答后可合成CRP。CRP是一种肝源性的急性蛋白,在巨噬细胞和T细胞分泌白介素6后增加,其生理作用是与死细胞或濒死细胞(和某些类型的细菌)表面的溶血磷脂胆碱结合,以通过C1Q复合物来激活补体系统。它与C-肽(胰岛素)或蛋白C(血凝)不相关。CRP通常作为炎症标志物,当机体受到炎症感染时,CRP水平会上升。最近的研究表明,患者具有较高基础水平的CRP,通常伴随着一个较高糖尿病、高血压和心血管疾病的发生率。
大鼠 Rat Crp 分子靶点信息
- 分子名:Crp, C-reactive protein
- 别称:
- Aa1249
- Ab1-341
- Ab2-196
- Ac1-114
- Ac1262
- Ac2-069
- Ba2-693
- C-reactive protein member of the pentraxin family
- C-reactive protein, member of the pentraxin family
- C-reactive protein, pentraxin-related
- C-reactive protein, petaxin related
- 基因序列:NCBI_Gene: 25419
- 蛋白序列:
- UniprotKB: A0A0G2K8V5
- UniprotKB: H6X2V2
- UniprotKB: H6X2V3
- UniprotKB: H6X2V4
- UniprotKB: H6X2V7
- UniprotKB: H6X2V8
- UniprotKB: H6X2V9
- UniprotKB: H6X2W0
- UniprotKB: H6X2W1
- UniprotKB: H6X2W2
- UniprotKB: H6X2W3
- UniprotKB: H6X2W4
- UniprotKB: H6X2W5
- UniprotKB: H6X2W6
- UniprotKB: H6X2W7
- UniprotKB: H6X2X0
- UniprotKB: P48199
- UniprotKB: Q63133
- UniprotKB: Q63134
大鼠 Rat Crp 靶点分子功能(预测)
Enables cholesterol binding activity; identical protein binding activity; and low-density lipoprotein particle binding activity. Involved in several processes, including cellular response to nitric oxide; complement activation, classical pathway; and negative regulation of superoxide anion generation. Located in extracellular space; filopodium; and growth cone. Used to study rheumatoid arthritis. Biomarker of several diseases, including acute kidney failure; artery disease (multiple); iron deficiency anemia; obesity; and status epilepticus. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; autoimmune disease (multiple); macular degeneration (multiple); middle cerebral artery infarction; and nephritis (multiple). Orthologous to human CRP (C-reactive protein).
引用文献统计
该产品被引用的文献总数为:14
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