Mouse Total MMP-9 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥2,000.00 – ¥3,400.00
在售SKU:70-EK2M09-48, 70-EK2M09-96, EK2M09
- 分子靶点:MMP9
- 种属:小鼠
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本5μL
- 灵敏度:34.9pg/mL
- 检测范围:0.16-10ng/mL
- 回收率:83%-124%
ELISA试剂盒详细信息
商品名 | 小鼠基质金属蛋白酶 9酶联免疫检测试剂盒 |
---|---|
种属 | 小鼠 |
靶点 | MMP9 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本5μL |
灵敏度 | 34.9pg/mL |
线性范围 | 0.16-10ng/mL |
精密度 | 板内变异系数:3.4%-3.7%;板间变异系数:2.8%-4.3% |
回收率 | 83%-124% |
平均回收率 | 95% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠totalMMP-9抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的totalMMP-9与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中totalMMP-9的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
MMP9 分子靶点信息概述
- 分子名:MMP9, matrix metallopeptidase 9
- 基因家族:M10 matrix metallopeptidases
- 曾用名:CLG4B
- 全称:matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
MMP9 分子靶点综述
基质金属蛋白酶9(MMP-9),又名92kDaIV型胶原酶、92kDa明胶酶或者明胶酶B,是一类属于锌-金属蛋白酶家族的酶。MMP-9参与正常生理过程(如胚胎发育、繁殖、血管形成、骨骼发育、伤口愈细胞迁移、学习和记忆)和病理过程(如关节炎、脑出血和肿瘤转移)的细胞外基质降解。MMP-9和弹性蛋白酶似乎是中性粒细胞迁移通过基底膜的调节因子。MMP-9在血管和新生血管形成中起重要作用。它在人类呼吸道上皮愈合过程中被显著上调,并被发现与许多病理过程相关,如癌症、免疫和心血管疾病。在类风湿性关节炎、局灶性脑缺血案例中可发现MMP-9的水平是升高的。它的水平随着特发性房颤的发展而增加。
小鼠 Mouse Mmp9 分子靶点信息
- 分子名:Mmp9, matrix metallopeptidase 9
- 别称:
- AW743869
- B/MMP9
- Clg4b
- collagenase IVB, basement membrane, 92 kDa
- expressed sequence AW743869
- Gel B
- Gelatinase B
- gelatinase B
- MMP-9
- 基因序列:NCBI_Gene: 17395
- 蛋白序列:
小鼠 Mouse Mmp9 靶点分子功能(预测)
Enables metalloendopeptidase activity. Involved in negative regulation of epithelial cell differentiation involved in kidney development and response to amyloid-beta. Acts upstream of or within several processes, including collagen catabolic process; embryo implantation; and extracellular matrix organization. Located in collagen-containing extracellular matrix. Is expressed in several structures, including alimentary system; central nervous system; early conceptus; limb mesenchyme; and skeleton. Human ortholog(s) of this gene implicated in several diseases, including Down syndrome; anodontia; artery disease (multiple); autoimmune disease (multiple); and lung disease (multiple). Orthologous to human MMP9 (matrix metallopeptidase 9).
引用文献统计
该产品被引用的文献总数为:13
- GSK-3α/β Activity Negatively Regulates MMP-1/9 Expression to Suppress Mycobacterium tuberculosis Infection
影响因子:8.786刊物:Frontiers in Immunology发表日期:2022/1/12 - Glucose and MMP-9 dual-responsive hydrogel with temperature sensitive self-adaptive shape and controlled drug release accelerates diabetic wound healing
影响因子:16.874刊物:Bioactive Materials发表日期:2022/1/19 - Local bone metabolism balance regulation via double-adhesive hydrogel for fixing orthopedic implants
影响因子:14.593刊物:Bioactive Materials发表日期:2021/10/19 - HMGB1-TLR4-IL23-IL17A axis promotes paraquat-induced acute lung injury by mediating neutrophil infiltration in mice
影响因子:4.259刊物:Scientific Reports发表日期:2017/4/4 - MiRNA-192-5p attenuates airway remodeling and autophagy in asthma by targeting MMP-16 and ATG7
影响因子:3.743刊物:BIOMEDICINE & PHARMACOTHERAPY发表日期:2019/12/30 - Metformin Ameliorates Inflammation and Airway Remodeling of Experimental Allergic Asthma in Mice by Restoring AMPKα Activity
影响因子:5.988刊物:Frontiers in Pharmacology发表日期:2022/1/24 - A non-Bactericidal Cathelicidin with Antioxidant Properties Ameliorates UVB-Induced Mouse Skin Photoaging via Intracellular ROS Scavenging and Keap1/Nrf2 Pathway Activation
影响因子:7.1刊物:FREE RADICAL BIOLOGY AND MEDICINE发表日期:2024-08-22 - Exploring the mechanism of Sendeng-4 against rheumacid arthritis through integrated serum pharmacochemistry, transcriptomics, and network pharmacology
影响因子:1.8刊物:BIOMEDICAL CHROMATOGRAPHY发表日期:2024-06-10 - Stomatin promotes neutrophil degranulation and vascular leakage in the early stage of severe burn injury via enhancement of the intracellular binding of neutrophil primary granules to F-actin
影响因子:2.7刊物:BURNS发表日期:2023-12-30 - Colchicine ameliorates short-term abdominal aortic aneurysms by inhibiting the expression of NLRP3 inflammasome components in mice
影响因子:5刊物:EUROPEAN JOURNAL OF PHARMACOLOGY发表日期:2023-12-20 - SPINT2 is involved in the proliferation, migration and phenotypic switching of aortic smooth muscle cells: Implications for the pathogenesis of thoracic aortic dissection
影响因子:2.7刊物:Experimental and Therapeutic Medicine发表日期:2023-10-09 - Influence of miR-142-3p on Pulmonary Fibrosis Through Regulation of p53/NF-κB
影响因子:0.2刊物:Journal of Pharmacology & Pharmacotherapeutics发表日期:2023-05-29 - Tailored Apoptotic Vesicle Delivery Platform for Inflammatory Regulation and Tissue Repair to Ameliorate Ischemic Stroke
影响因子:17.1刊物:ACS Nano发表日期:2023-04-26