Mouse myeloperoxidase/MPO ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
在售SKU:70-EK2133/2-48, 70-EK2133/2-96, EK2133
- 分子靶点:MPO
- 种属:小鼠
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本5μL
- 灵敏度:4.40pg/mL
- 检测范围:62.5-4000pg/mL
- 回收率:91%-107%
ELISA试剂盒详细信息
商品名 | 小鼠髓过氧化物酶酶联免疫检测试剂盒 |
---|---|
种属 | 小鼠 |
靶点 | MPO |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本5μL |
灵敏度 | 4.40pg/mL |
线性范围 | 62.5-4000pg/mL |
精密度 | 板内变异系数:3.8%-5.4%;板间变异系数:3.0%-4.1% |
回收率 | 91%-107% |
平均回收率 | 99% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠MPO抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的MPO与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中MPO的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
MPO 分子靶点信息概述
- 分子名:MPO, myeloperoxidase
MPO 分子靶点综述
髓过氧化物酶(MPO)是一种过氧化物酶,在人类中由位于17号染色体上的MPO基因编码。它是一种存在于中性粒细胞的嗜苯胺蓝颗粒中的溶酶体蛋白,在脱颗粒过程中释放到细胞外空间。MPO在中性粒细胞中表达最丰富,可产生具有抗菌活性的次卤酸。MPO有血红素色素,导致中性粒细胞中的分泌物呈现绿色,如脓。另外,MPO介导蛋白质亚硝基化、3-氯酪氨酸形成及二酪氨酸交联。MPO缺乏症是一种导致机体免疫缺陷的遗传性疾病。MPO水平的升高会大大加倍增加心血管死亡的风险。也有人认为,MPO在动脉粥样硬化病变发展中、致使斑块不稳定中起着重要作用。
小鼠 Mouse Mpo 分子靶点信息
- 分子名:Mpo, myeloperoxidase
- 别称:
- 无
- 基因序列:NCBI_Gene: 17523
- 蛋白序列:
小鼠 Mouse Mpo 靶点分子功能(预测)
Enables peroxidase activity. Acts upstream of or within several processes, including hypochlorous acid biosynthetic process; removal of superoxide radicals; and response to fungus. Located in mitochondrion. Is expressed in several structures, including alimentary system; genitourinary system; hemolymphoid system; integumental system; and skeleton. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer's disease 1; cystic fibrosis; kidney failure (multiple); liver disease (multiple); and lung disease (multiple). Orthologous to human MPO (myeloperoxidase).
引用文献统计
该产品被引用的文献总数为:75
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