Mouse IL-23 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
在售SKU:70-EK223-48, 70-EK223-96, EK223
- 分子靶点:IL23A
- 种属:小鼠
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本20μL
- 灵敏度:0.31pg/mL
- 检测范围:1.56-100pg/mL
- 回收率:80%-99%
ELISA试剂盒详细信息
商品名 | 小鼠白介素23酶联免疫检测试剂盒 |
---|---|
种属 | 小鼠 |
靶点 | IL23A |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本20μL |
灵敏度 | 0.31pg/mL |
线性范围 | 1.56-100pg/mL |
精密度 | 板内变异系数:3.0%-5.4%;板间变异系数:4.4%-6.4% |
回收率 | 80%-99% |
平均回收率 | 91% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-23抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-23与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-23的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
IL23A 分子靶点信息概述
- 分子名:IL23A, interleukin 23 subunit alpha
- 基因家族:Interleukins
- 别名:SGRF; IL23P19; IL-23; IL-23A; P19
- 全称:interleukin-six, G-CSF related factor; interleukin 23, alpha subunit p19
IL23A 分子靶点综述
白细胞介素23(IL-23)是一种由IL-12p40亚基和IL-23p19亚基组成的异二聚体细胞因子,其中p40亚基是IL-23与IL-12共用的。IL-23的功能性受体已被鉴定,由IL-12Rβ1和IL-23R组成。IL-23在1型极化T细胞的免疫应答中起作用。虽然IL-12可强有力激活幼稚T细胞,但是最初的报道认为IL-23可优先作用于记忆T细胞,促进其分泌IFN-γ和增殖,这表明IL-23在控制细菌感染上有重要作用。IL-23被进一步描述为是一种控制周围组织炎症的关键细胞因子。p19的过表达与多个器官以及包含皮肤在内的上皮组织的炎症有关。IL-23参与中枢神经系统和自身免疫性疾病的炎症。
小鼠 Mouse Il23a 分子靶点信息
- 分子名:Il23a, interleukin 23, alpha subunit p19
- 别称:
- IL-23
- IL-23p19
- p19
- 基因序列:NCBI_Gene: 83430
- 蛋白序列:
小鼠 Mouse Il23a 靶点分子功能(预测)
Contributes to interleukin-23 receptor binding activity. Involved in positive regulation of defense response to virus by host; positive regulation of immune response; and positive regulation of neutrophil chemotaxis. Acts upstream of or within T cell proliferation and positive regulation of transcription by RNA polymerase II. Located in extracellular space. Part of interleukin-23 complex. Is expressed in decidua; decidua basalis; embryo; and extraembryonic component. Orthologous to human IL23A (interleukin 23 subunit alpha).
引用文献统计
该产品被引用的文献总数为:25
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