Human TNF-β/Lymphotoxin-α ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
在售SKU:70-EK1139-48, 70-EK1139-96, EK1139
- 分子靶点:LTA
- 种属:人
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本10μL
- 灵敏度:2.02pg/mL
- 检测范围:62.5-4000pg/mL
- 回收率:87%-113%
ELISA试剂盒详细信息
商品名 | 人肿瘤坏死因子β/淋巴毒素α酶联免疫检测试剂盒 |
---|---|
种属 | 人 |
靶点 | LTA |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本10μL |
灵敏度 | 2.02pg/mL |
线性范围 | 62.5-4000pg/mL |
精密度 | 板内变异系数:3%-6.2%;板间变异系数:3.5%-4.7% |
回收率 | 87%-113% |
平均回收率 | 102% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人TNF-β抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的TNF-β与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中TNF-β的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
分子信息
LTA 分子靶点信息概述
- 分子名:LTA, lymphotoxin alpha
- 基因家族:Tumor necrosis factor superfamily
- 别名:TNFSF1; LT
- 曾用名:TNFB
- 全称:TNF superfamily member 1; lymphotoxin alpha (TNF superfamily, member 1)
LTA 分子靶点综述
肿瘤坏死因子β(TNF-β),又称为淋巴毒素α,是由淋巴细胞分泌的细胞因子。TNF-β介导许多炎症、免疫刺激和抗病毒应答。在发育阶段,TNF-β还参与二级淋巴器官的形成,并在凋亡中发挥作用。在TNF-β敲除小鼠中,所有派伊尔氏淋巴集结和淋巴结都无法发育,这表明了TNF-β在免疫发育中的重要性。
人 Human LTA 分子靶点信息
- 分子名:LTA, lymphotoxin alpha
- 别称:
- CDF
- cholinergic differentiation factor
- D factor
- DIA
- differentiation inhibitory activity
- differentiation stimulating factor
- differentiation-inducing factor
- differentiation-stimulating factor
- hepatocyte-stimulating factor III
- HILDA
- human interleukin in DA cells
- leukemia inhibitory factor
- LIF, interleukin 6 family cytokine
- melanoma-derived LPL inhibitor
- MLPLI
- 基因序列:NCBI_Gene: 4049
- 蛋白序列:UniProtKB: P01374
人 Human LTA靶点分子功能(预测)
Enables cytokine activity; growth factor activity; and leukemia inhibitory factor receptor binding activity. Involved in several processes, including leukemia inhibitory factor signaling pathway; positive regulation of protein modification process; and regulation of epithelial cell differentiation involved in kidney development. Acts upstream of or within several processes, including positive regulation of MAPK cascade; positive regulation of macrophage differentiation; and positive regulation of protein phosphorylation. Located in cytosol.
引用文献统计
该产品被引用的文献总数为:2
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