EK9137
Human/Rat PDGF-BB ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
在售SKU:70-EK9137-48, 70-EK9137-96, EK9137
- 种属:人、大鼠
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本10μL
- 灵敏度:2.13pg/mL
- 检测范围:31.25-2000pg/mL
- 回收率:82%-116%
ELISA试剂盒详细信息
商品名 | 人/大鼠血小板衍生因子BB酶联免疫检测试剂盒 |
---|---|
种属 | 人、大鼠 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本10μL |
灵敏度 | 2.13pg/mL |
线性范围 | 31.25-2000pg/mL |
精密度 | 板内变异系数:2.3%-4.5%;板间变异系数:3.3%-4.9% |
回收率 | 82%-116% |
平均回收率 | 90% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人/大鼠PDGF-BB抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的PDGF-BB与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中PDGF-BB的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
引用文献统计
该产品被引用的文献总数为:14
- The conditioned medium from mesenchymal stromal cells pretreated with proinflammatory cytokines promote fibroblasts migration and activation
影响因子:3.752刊物:PLoS One发表日期:2022/4/11 - The phenotype of vascular smooth muscle cells co-cultured with endothelial cells is modulated by PDGFR-β/IQGAP1 signaling in LPS-induced intravascular injury
影响因子:2.333刊物:International Journal of Medical Sciences发表日期:2019/8/6 - Proximity hybridization-induced on particle DNA walker for ultrasensitive protein detection
影响因子:5.256刊物:ANALYTICA CHIMICA ACTA发表日期:2019/5/8 - Chondroprotective effects of platelet lysate towards monoiodoacetate-induced arthritis by suppression of TNF-α-induced activation of NF-ĸB pathway in chondrocytes
影响因子:5.515刊物:Aging-US发表日期:2019/5/14 - Growth factors-based platelet lysate rejuvenates skin against ageing through NF-κB signalling pathway: In vitro and in vivo mechanistic and clinical studies
影响因子:8.755刊物:CELL PROLIFERATION发表日期:2022/3/11 - Macrophage Polarization Mediated by Chitooligosaccharide (COS) and Associated Osteogenic and Angiogenic Activities
影响因子:4.152刊物:ACS Biomaterials Science & Engineering发表日期:2020/1/16 - Advanced-platelet-rich fibrin extract promotes adipogenic and osteogenic differentiation of human adipose-derived stem cells in a dose-dependent manner in vitro
影响因子:2.466刊物:TISSUE & CELL发表日期:2021/2/5 - Growth factors-based beneficial effects of platelet lysate on umbilical cord-derived stem cells and their synergistic use in osteoarthritis treatment
影响因子:6.304刊物:Cell Death & Disease发表日期:2020/10/14 - IRF1/ZNF350/GPX4-mediated ferroptosis of renal tubular epithelial cells promote chronic renal allograft interstitial fibrosis
影响因子:8.101刊物:FREE RADICAL BIOLOGY AND MEDICINE发表日期:2022/11/7 - Ferroptosis contributes to endometrial fibrosis in intrauterine adhesions
影响因子:7.4刊物:FREE RADICAL BIOLOGY AND MEDICINE发表日期:2023-06-10 - The Role of Macrophage Phenotype in the Vascularization of Prevascularized Human Bone Marrow Mesenchymal Stem Cell Sheets
影响因子:4刊物:STEM CELLS AND DEVELOPMENT发表日期:2023-04-29 - Duo-role Platelet-rich Plasma: temperature-induced fibrin gel and growth factors’ reservoir for microneedles to promote hair regrowth
影响因子:10.7刊物:Journal of Advanced Research发表日期:2023-02-26 - Proximity Hybridization-Induced on Particle DNA Walker for Ultrasensitive Protein Detection
刊物:Analytica chimica acta 1074, 142-149 (2019) - Simvastatin Preparations Promote Pdgf-Bb Secretion to Repair Lps-Induced Endothelial Injury through the Pdgfrβ/Pi3k/Akt/Iqgap1 Signalling Pathway
刊物:Journal of cellular and molecular medicine 23, 8314-8327 (2019)