EK1368

Human P4HB ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

分子靶点：P4HB, PO4DB, ERBA2L
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：7.77 pg/mL
检测范围：0.16 ng/mL - 10 ng/mL
回收率：77% - 104%

描述

文章目录[隐藏]

分子信息
P4HB 分子靶点信息概述
P4HB 分子靶点综述
- 人 Human P4HB 分子靶点信息
- 人 Human P4HB靶点分子功能（预测）

商品名	Human P4HB ELISA Kit (人脯氨酸4-羟化酶β亚基 (P4HB) ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：2.5% - 3.9%；板间变异系数：1.6%-6.2%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	7.77 pg/mL
检测范围	0.16 ng/mL - 10 ng/mL
回收率	77% - 104%
平均回收率	0.93
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗P4HB单克隆抗体的96孔聚苯乙烯酶标板人P4HB冻干标准品人P4HB检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人P4HB预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和辣根过氧化物酶标记的检测抗体，经过孵育，样本中存在的P4HB与固相抗体和检测抗体结合。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中P4HB的浓度成正比。加入终止液终止反应，在450 nm波长 (参考波长570 - 630 nm)测定吸光度值。

Human P4HB ELISA Kit (人脯氨酸4-羟化酶β亚基 (P4HB) ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 P4HB, PO4DB, ERBA2L 靶点信息

P4HB 分子靶点信息概述

分子名：P4HB, prolyl 4-hydroxylase subunit beta
基因家族：Protein disulfide isomerases
别名：PDIA1; PROHB; DSI; GIT; PDI; PO4HB; P4Hbeta
曾用名：PO4DB; ERBA2L
全称：protein disulfide isomerase-associated 1; protein disulfide isomerase family A, member 1; collagen prolyl 4-hydroxylase beta; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide (protein disulfide isomerase; thyroid hormone binding protein p55); procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide (protein disulfide isomerase-associated 1); procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; prolyl 4-hydroxylase, beta polypeptide

P4HB 分子靶点综述

蛋白质二硫键异构酶，又称脯氨酸4-羟化酶β亚基(P4HB)、细胞甲状腺激素结合蛋白(p55)和谷胱甘肽-胰岛素转氢酶，是一种外周膜蛋白，属于蛋白二硫键异构酶家族。它包含两个硫氧还蛋白结构域，催化二硫键的形成、断裂和重排。P4HB定位于淋巴细胞表面CD4富集区附近。在黑素小体组分中，P4HB可降低并激活HIV-1 gp120表面蛋白的融合特性，从而使HIV-1进入细胞。P4HB作为一种伴侣，抑制错误折叠蛋白的聚集。P4HB可能与其它分子伴侣参与了激素生物合成中TG前体的结构修饰。

人 Human P4HB 分子靶点信息

分子名：P4HB, prolyl 4-hydroxylase subunit beta
别称：
- cellular thyroid hormone-binding protein
- CLCRP1
- collagen prolyl 4-hydroxylase beta
- DSI
- ERBA2L
- GIT
- glutathione-insulin transhydrogenase
- P4Hbeta
- p55
- PDI
- PDIA1
- PHDB
- PO4DB
- PO4HB
- procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide
- PROHB
- prolyl 4-hydroxylase, beta polypeptide
- protein disulfide isomerase family A, member 1
- protein disulfide isomerase-associated 1
- protein disulfide isomerase/oxidoreductase
- protein disulfide-isomerase
- protocollagen hydroxylase
- testicular secretory protein Li 32
- thyroid hormone-binding protein p55
- v-erb-a avian erythroblastic leukemia viral oncogene homolog 2-like
基因序列：NCBI_Gene: 5034
蛋白序列：UniProtKB: P07237

人 Human P4HB靶点分子功能（预测）

Enables several functions, including oxidoreductase activity, acting on a sulfur group of donors; protein disulfide isomerase activity; and protein heterodimerization activity. Contributes to procollagen-proline 4-dioxygenase activity. Involved in several processes, including positive regulation of substrate adhesion-dependent cell spreading; positive regulation of viral entry into host cell; and protein folding in endoplasmic reticulum. Acts upstream of or within peptidyl-proline hydroxylation to 4-hydroxy-L-proline. Located in several cellular components, including endoplasmic reticulum-Golgi intermediate compartment; external side of plasma membrane; and lamellipodium. Part of procollagen-proline 4-dioxygenase complex. Implicated in Cole-Carpenter syndrome.